Resource data sheet


Expression vector of V5-tagged human NECAB3 gene; wild type.

Catalog number RDB14306
Resource name pcDNA3.1_V5-NECAB3_WT
Clone info. Expression vector of V5-tagged human NECAB3 gene; wild type, see Fig. 5A for details.
Expression was confirmed by the depositor with immunoprecipitation and real-time PCR.
[Data sheet:]
Vector backbone pcDNA3 derivative
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Classification Plasmid
Type_of_insert fragment human cDNA (HT1080 human fibrosarcoma cell (ATCC))
Deposotor's page Sakamoto, Takeharu |
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakaoka, H.J. et al., Sci. Rep. 6: 22784, 2016)
(Japanese text [open/close])
Fee (non-profit org.)

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

original - reference Nakaoka, H.J., NECAB3 Promotes Activation of Hypoxia-inducible factor-1 during Normoxia and Enhances Tumourigenicity of Cancer Cells. Sci. Rep, 6: 22784 (2016).
PMID 26948053.