Resource data sheet
Prev.

pET28/His6-EGFP-Nakanori

Marker to localize sphingomyelin and cholesterol lipid domains. Expression vector of His and EGFP-tagged G. frondosa Nakaniori for E. coli.

Catalog number RDB14301
Resource name pET28/His6-EGFP-Nakanori
Clone info. Expression vector of His and EGFP-tagged G. frondosa Nakaniori for E. coli. Nakanori is non-toxic mushroom protein that specifically binds a complex of sphingomyelin.
Expression was confirmed by the depositor with SDS-PAGE and confocal micriscopy. AB700589.1 for Grifola frondosa Nakanori mRNA for hypothetical protein, complete cds.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name Grifola frondosa Nakanori cDNA
Depositor Sako, Yasushi | Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A. et al., FASEB J. 2016 Aug 4. pii: fj.201500075R.)
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Remarks ((Additional form)) GFP-A
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14301 pET28/His6-EGFP-Nakanori DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14301_A6Hup1_2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB14301_A6Hua.seq
>D03762A2_A6Hu_2_T7_20bp_E11_14_ABI24.ab1
    1 TCGGGTCATT CCCATCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGG
   61 CAGCAGCCAT CATCATCATC ATCACAGCAG CGGCCTGGTG CCGCGCGGCA GCCATATGGC
  121 TAGAGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
  181 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA
  241 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
  301 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
  361 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT
  421 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
  481 GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA
  541 CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA
  601 CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC
  661 CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA
  721 CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT
  781 CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGAA
  841 GCTTGGAGGA GGAGGAGGAG AATTCATGCT TTACGGCGTC GAGATAGACG AGCAGTACCT
  901 GCGCGTGATG GAGGAGTACA AAGGACAAGG AAGTAATCAC GCAGGCCGAC ATGGCCCAAG
  961 GTCGCGCTAC AACGCAAGAA CGTGTACCAG GGACCAGGCC GAGAAGCGCC AGGCTGAGCT
 1021 TTAAAGCCCG AATATGGAGT CGTGTCTGTG TCCTCGTGCG TGTGTACATG CTACTGGTGG
 1081 GCCCGATCAC AAGCCGAGAT CGAAGAAGTT CTTTCGAGGC CAACTTGGCG GCGCACAACC
1141 T
//
Primer: T7_terminator RDB14301_A6Hub.seq
>D03762A2_A6Hu_2_T7 Terminator_F11_17_ABI24.ab1
    1 GGCTCGCCTT TCTTTTCGGG CTTTGTTAGC AAGCCGGATC TCAGTGGTGG TGGTGGTGGT
   61 GGGATCCTCA ACGGGAGTAC ACATACTCCA CGTAAGCGGT AGTCTCATTT CCAGTCGACC
  121 CGTGAGCCTT GACATCGCCG TAGCTCGAGT CGCTATTCTG GCCGTTGTGC GTGTCGAGTA
  181 GGTGGAGCAT ATAGTCCTTG CTACCGTTCT TCCACCAATG ACCGCTCTGG CGCACCTCAA
  241 CGAGCACCTG AGAATCCCAC GAGCGGTTCC AGGGGTTCTG CCAGCCTGAG AAGATGTCGA
  301 GGTTGTCCGT GGTGCCATAC ACGATGCACC CCGCGGACCC GACTGCGGCG CCCGCCGACT
  361 TGGTGTGGAT GAACACCGTC CATTGCCCAT TCCCGATGCG CTTTTCTCGA GTGTGCGCGC
  421 CAAAGTGGCC TCGGAAGGAC TCCTCGATCT TCGCTGTGAT CGGGCCGCCA GTAGCATTGT
  481 ACACACGCAC GAGGACACAG ACACCGACTC CATATTCGGC CTTAAGCTCA GCCTGGCGCT
  541 TCTCGGCCTG GTCCTGGTAC ACGTTCTTGC GTTGTAGCGC GACCTTGGCC ATGTCGGCCT
  601 GCGTGATTAC TTCCTTGTCC TTGTACTCCT CCATCACGCG CAGGTACTGC TCGTCTATCT
  661 CGACGCCGTA AAGCATGAAT TCTCCTCCTC CTCCTCCAAG CTTCTTGTAC AGCTCGTCCA
  721 TGCCGAGAGT GATCCCGGGC GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC
  781 TCGTTGGGGT CTTTGCTCAG GGCGGGACTG GGGTGCTCAG GTAGTGGTTG TCGGGCAGCA
  841 GCACGGGGGC CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG
  901 CCGTCCTCGA TGTTTGTGGC GGGATCTTGA AGTTCACCTT GATGCCCGTT TCTTTCTGCT
  961 TGTCGGCCAT GATATAGACG TTTGTGGCTG TTTGTAGTTG TACTTCAAGC TTGTGCCCCA
 1021 AGGATGTTGG CCGTTCCTCC TTTGAAGTCG AATGCCCTTT CAGCCTCGAT GCGGATTTCA
 1081 CCCAGGGTGT TCGCCCTCGG ACTTTCAACC CTTCCGAGCG CGGGTCTTTG TAGTTGACGG
1141 TTCGTTCCTT GAAAAAAGAT TGGGTCCGCC CTTCCCTGTG AG
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Dnaconda's recommendation EXR0062j (Japanese text)
Featured content Sphingolipid signaling pathway (English text)

References

original Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 2016 Aug 4. pii: fj.201500075R. [Epub ahead of print] PMID 27492925.

2017.07.18

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