Resource data sheet
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pGimRET

Expression vector of GimRET (Glycine inserted mutant FRET probe) for mammalian cells.

Catalog number RDB14204
Resource name pGimRET
Clone info. Expression vector of glycine-inserted mutant FRET (GimRET) fusion protein for Mammalian cells. GimRET works as a protein-crowding sensor that consists of CFP with YFP1G (one glycine insertion).
Expression was confirmed by the depositor with microsopy. YFP1G (Inserted a Gly residue in the position of the 145th Tyr residue).
Vector backbone pECFP-N1 (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Kan^r (E. coli), Neo^r (mammaliancells)
Gene/insert name Aequorea victoria GFP cDNA
Depositor Watanabe, Tomonobu M. |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Morikawa, T.J. et al., Sci. Rep. 6: 22342, 2016)
(Japanese text [open/close])
Remarks ((Additional form)) GFP-A
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14204 pGimRET DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14204_A6Fop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14204_A6Foa.seq
>D03467A2_A6Fo_1_CMV-Forward_H10_22.ab1
    1 ACTTAATCAC TCCGGCCCCA TTGACGCAAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA
  121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
  181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
  241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
  301 TCGTGACCAC CCTGACCTGG GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
  361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
  421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
  481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
  541 AGCTGGAGTA CAACTACATC AGCCCACAAC GTCTATATCA CCGCCGACAA GCAGAACAAC
  601 GGCATCAAGG CCAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC
  661 GACCACTACC AGCAGAACAC CCCCATCAGC GACGGCCCCG TGCTGCTGCC CGATAACCAC
721 TACCTGAGCA CCCAGTCAGC CCTGAGCAAA GACCA
//
Primer: pGL3_RV5F RDB14204_A6Fob.seq
>D03467A2_A6Fo_1_pGL3_RV5F_A03_03.ab1
    1 ACCCATAAAA TGTGGTATGG CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC
   61 CCGCGGTACC GTCGACTGCA GAATTCGAAG CTTGAGCTCG AGTTACTTGT ACAGCTCGTC
  121 CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT
  181 CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GTAGCTCAGG TAGTGGTTGT CGGGCAGCAG
  241 CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC
  301 GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT GCTTGTCGGC
  361 CATGATATAG ACGTTGTGGC TGTTGTATCC GTTGTACTCC AGCTTGTGCC CCAGGATGTT
  421 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
  481 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC
  541 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
  601 GTAGCGGGCG AAGCACTGCA GGCCGTAGCC GAAGGTGGTC ACGAGGGTGG GCCAGGGCAC
  661 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC
  721 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG
  781 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACACCAGTTC CACCTGAGCC
  841 GCCTGAACCT CCAGTACCGC TGGTGCTGCC TCCGGACTTG TACAGCTCGT CCATGCCGAG
  901 AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG
  961 GGTCTTTGCT CAGGGCGGAC TGGGGTGCTC AAGGTAGTGG GTTGTCGGGC AGCAGCACGG
 1021 GGGCCCGTCG CCCGATGGGG GGGTGTTCTG CTGGTAGTGG GTCGGCGAGC TGCACGCTGC
 1081 CGGTCCTCGA ATGGTGTGCG GGAATCCTTG GAGTTGGCCT TGGAATGCCG GATTCTTCTC
1141 CTGTGCCT
//
Primer: SV40pro_F_V2 RDB14204_A6Foc.seq
>D03467A2_A6Fo_1_SV40pro_F_V2_B11_05.ab1
    1 CGGAAACACT CCGCTGCATT GTCGCCTTGG CCGGATCGAT CTGTACAGAG GATGATGATC
   61 GTTTCCCAAG ATTGAACAGA TGGATTGCAC ATCCGTTCTC CGGCCGCTTG GGTGGATAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCACC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCCATCA GGATGATCTG
  541 GACTAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCAATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCCGCTA
  721 TCACGACATA GCGTTGGCTA CCCGTGATAT TGCTGAACAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTTCCT CCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  841 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT TCGAAATGAC CGACCAAGCG
  901 ACGCCCAACC TGCCATCACG AGATTTCCAT TTCCACGTCG CCTTCCTATG AAAGGTGGGC
  961 TTCGGAATCG TTTTCCGGGG ACGCCGAGCT GGATGATCCT CCAGCGCGGG GGGATTCTCA
 1021 TGACTTGGAG TTTCTTCGCC CACCCATACG GGGGGGAGGG CTATATGTGA ACACCACTGT
1081 AAAAG
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Dnaconda's recommendation EXR0059j (Japanese text)

References

original Konishi, H.A., In vivo analysis of protein crowding within the nuclear pore complex in interphase and mitosis. Sci. Rep. 7 (1): 5709 (2017). PMID 28720791.
original Morikawa, T.J., Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it. Sci. Rep. 6: 22342 (2016). PMID 26956628.

2017.08.30

GNP_filter3_RDBDEP_html_170829.pl