Resource data sheet
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pPAL7_GimRET

Expression vector of GimRET (Glycine inserted mutant FRET probe) for E. coli.

Catalog number RDB14203
Resource name pPAL7_GimRET
Alternative name CFP-YFP1G GimRET
Clone info. Expression vector of glycine-inserted mutant FRET (GimRET) fusion protein for E.coli. GimRET works as a protein-crowding sensor that consists of CFP with YFP1G (one glycine insertion).
YFP1G (Inserted a Gly residue in the position of the 145th Tyr residue).
Vector backbone pAL7 (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Watanabe, Tomonobu M. |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Morikawa, T.J. et al., Sci. Rep. 6: 22342, 2016)
(Japanese text [open/close])
Remarks ((Additional form)) GFP-A
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14203 pPAL7_GimRET DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14203_A6Fop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: AFR RDB14203_A6Foa.seq
>D03467A1_A6Fo_1_AFR_E02_14.ab1
    1 CCCATCTTTG TTTACTTTAA GAGGAGATAT ACATATGGGA GGGAAATCAA ACGGGGAAAA
   61 GAAATATATT GTCGGGTTCA AACAGGGCTT TAAGAGCTGC GCTAAGAAGG AGGATGTCAT
  121 TTCTGAAAAA GGCGGGAAAC TCCAAAAGTG CTTCAAATAT GTAGACGCAG CTAGCGCTAC
  181 ATTAAACGAA AAAGCTGTAG AAGAATTGAA AAAAGATCCG AGCGTCGCGT ACGTAGAAGA
  241 AGACAAGCTC TTCAAAGCTT TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC
  301 CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG
  361 CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT
  421 GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTGG GGCGTGCAGT GCTTCAGCCG
  481 CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT
  541 CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA
  601 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA
  661 CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACATC AGCCACAACG TCTATATCAC
  721 CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA ACATCGAGGA
  781 CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT
  841 GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA
  901 GAAGCGCGAT CACATGGGTC CCTGCTGGAG TCGTGACCGC CGCCGGATCA CTCTCGGGCA
  961 TGGACGAGCT GTACAGTCCG GAGGCAGCCA CCAAGCGTTA CTGCAGCTCA GGCGGCTCAA
 1021 GGTGACTTGG TGTGAGCAAG GGCGAAGGAA CCTGGTTCAC GGGTTGATTG CATCCTATCG
1081 AACTTGACGG CGAACGATAA CACCGGTCTC T
//
Primer: T7 terminator RDB14203_A6Fob.seq
>D03467A1_A6Fo_1_T7Terminator_A12_03.ab1
    1 GACGGCATCT TTCGGGCTTA TGCGGCCGCC TCGAGTTACT TGTACAGCTC GTCCATGCCG
   61 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG
  121 GGGTCTTTGC TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG
  181 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG
  241 ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC GGCCATGATA
  301 TAGACGTTGT GGCTGTTGTA TCCGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC
  361 TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC
  421 ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG
  481 TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG
  541 GCGAAGCACT GCAGGCCGTA GCCGAAGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC
  601 TTGCCGGTGG TGCAGATGAA CTTCAGGGTC AGCTTGCCGT AGGTGGCATC GCCCTCGCCC
  661 TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC
  721 ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACACCAG TTCCACCTGA GCCGCCTGAA
  781 CCTCCAGTAC CGCTGGTGCT GCCTCCGGAC TTGTACAGCT CGTCCATGCC GAGAGTGATC
  841 CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGGTCTTT
  901 GCTCAGGGCG GACTGGGTGC TCAGGTAGTG GGTTGTCGGG GCAGCAGCAC GGGGGCCGTC
  961 GCCGATGGGG GGTGTTTCTG CTGGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCCTCGA
 1021 TGTTTGTGGC GGGATCTTGA AGTTTGGGCC CTTGATGCCG ATTCCTCCTG CCTTGTCGGC
1081 CGATTGATAT AGACGGTGGT GGCTGCATGT AGTTGGTTAA CCTTCCCAGG C
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Dnaconda's recommendation EXR0059j (Japanese text)

References

original Konishi, H.A., In vivo analysis of protein crowding within the nuclear pore complex in interphase and mitosis. Sci. Rep. 7 (1): 5709 (2017). PMID 28720791.
original Morikawa, T.J., Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it. Sci. Rep. 6: 22342 (2016). PMID 26956628.

2017.08.30

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