Resource data sheet
Prev.

pEGFP-C1-mouse Rab35

Expression vector of EGFP-tagged mouse Rab35.

Catalog number RDB14197
Resource name pEGFP-C1-mouse Rab35
Clone info. Expression vector of EGFP-tagged mouse Rab35.
Expression was confirmed by the depositor with co-immunoprecipitation.
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB14197]
Vector backbone pEGFP-C1
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^R (mammalian cells).
Classification Plasmid
Type_of_insert fragment mouse cDNA ()
Other clones with this gene 77407 | (indicated in NCBI Gene ID)
Deposotor's page Fukuda, Mitsunori |
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Etoh, K. and Fukuda, M., J. Biol. Chem. 290:9064-9074, 2015, Mrozowska, P. S. and Fukuda, M., J. Cell Biol. 213, 3, 355-369, 2016).
(Japanese text [open/close])
Remarks ((Additional form)) GFP-A
(Japanese text [open/close])
Fee (non-profit org.)

We examined.

Data are summerized on test sheet below.

Test sheet RDB14197_A6Fdp1_2.pdf

Nucleotide sequence of a portion of this resource.

Primer: EGFP-C RDB14197_A6Fda_2.seq
>D03515A1_A6Fd_1_EGFP-C_B04_04.ab1
    1 CAGCATCTCG GCATGGACGA GCTGTACAGT CCGGACTCAG ATCCATGGCC CGGGACTACG
   61 ACCACCTCTT CAAGCTGCTC ATCATCGGCG ACAGCGGTGT GGGCAAGAGC AGCTTGCTGT
  121 TACGATTCGC AGACAACACC TTCTCAGGCA GCTACATCAC CACAATCGGA GTGGATTTCA
  181 AGATTCGGAC TGTGGAGATC AACGGGGAGA AGGTGAAGCT GCAGATCTGG GACACGGCAG
  241 GGCAGGAGCG CTTCCGCACC ATCACCTCTA CGTATTATCG GGGGACCCAT GGGGTCATTG
  301 TGGTTTACGA CGTCACTAGT GCCGAGTCCT TTGTCAACGT CAAGCGATGG CTTCATGAAA
  361 TCAACCAGAA CTGTGACGAT GTGTGCCGAA TATTAGTGGG CAATAAGAAT GATGACCCTG
  421 AGCGGAAGGT GGTAGAGACA GAAGATGCCT ACAAATTTGC CGGGCAGATG GGGATCCAGC
  481 TCTTTGAGAC CAGTGCCAAG GAGAACGTCA ATGTGGAAGA GATGTTCAAC TGTATCACAG
  541 AGCTGGTTCT GCGAGCAAAG AAAGACAACT TGGCGAAACA GCAGCAGCAA CAACAGAACG
  601 ATGTGGTGAA GCTCACCAAA AACAGTAAAC GAAAGAAACG CTGCTGCTAA GTCGACGGTA
  661 CCGCGGGCCC GGGATCCACC GGATCTAGAT AACTGATCAT AATCAGCCAT ACCACATTTG
  721 TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA
  781 TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  841 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTTCAC TGCATTCTAG TTGTGGTTTG
  901 TCCAAACTCA TCAATGTATC TTTAACGCGT AATTTGTAAG CGTTAAATAT TTTGTTAAAA
  961 TTTCGCGTTT AAATTTTTGT AAATCAGCCT CATTTTTAAC CCAATAGACG AAATCCGGCC
 1021 AAATCCATAT AATTCAAAGG ATTAGGACCG AGATCGGATT GATGTATGAT CAAGCTTTTG
1081 CATCAG
//
Primer: CMV-Forward RDB14197_A6Fdb_2.seq
>D03515A1_A6Fd_1_CMV-Forward_A04_01.ab1
    1 AGTACTCGCC CATTGACGCA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA
   61 GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA TGGTGAGCAA
  121 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
  181 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC
  241 CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
  301 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT
  361 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
  421 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
  481 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
  541 CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT
  601 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA
  661 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC
  721 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT
  781 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC
  841 CATGGGCCCG GGACTACGAC CACCTCTTTC AAGCTGCTCA TCATCGGGCG ACAGCGGGTG
  901 TGGGCAAGAG CAGCCTTGCT GTTACGATTC GCAGACAACA CCATTCTCAG CAGCTACATC
  961 ACCACATCGG GAGTGATTTC AGATTCGGAA CTGTGGAGAT CCACGGGGGA GAGTGAAGCC
1021 TGCCAGATCT GGCACCGTAG TCAGAGCGCT TCCGCACAAA TCAAT
//
Primer: SV40pro_F_V2 RDB14197_A6Fdc_2.seq
>D03515A1_A6Fd_1_SV40pro_F_V2_D04_10.ab1
    1 GTTAGGGCTT TTTGGGAGCC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC
  901 GCCCAACCTG CCATCACGAG ATTTCGATTT CCACCGCCCG CCTTCTATGA AAGGTTGGGC
  961 TTTCGGAAAT CGTTTTTCCG GGACGCCGGC TGGGATGAAT CCTCCCAGCC GCGGGATCTC
 1021 ATGCTGGGAG TTCCTTCGCC CAACCCTTAG GGGAGGCTTA CTTGAAACCA CGGTATGGAG
1081 CACTAGTAC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

original - reference Mrozowska, P.S., Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. J. Cell Biol. 213, 3, 355-369 (2016). PMID 27138252.
original - reference Etoh, K., Structure-function analyses of the small GTPase Rab35 and its effector protein centaurin-beta2/ACAP2 during neurite outgrowth of PC12 cells. J. Biol. Chem. 290 (14): 9064-9074 (2015). PMID 25694427.

2017.01.05

GNP_filter3_RDBDEP_html_160913.pl