Resource data sheet
Prev.

pEF-HERC2

Expression vector of human HERC2.

Catalog number RDB13974
Resource name pEF-HERC2
Clone info. Expression vector of human HERC2.

[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB13974]
Vector backbone pEF-BOS
Size of vector backbone 5.3 kb
Selectable markers Amp^r
Classification Plasmid
Type_of_insert fragment human cDNA (HEK293T)
Other clones with this gene 8924 | (indicated in NCBI Gene ID)
Deposotor's page Imai, Yuzuru
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)
(In Japanese [open/close])
Fee (non-profit org.)

We verified.

Data are summerized on test sheet below.

Test sheet RDB13974_A6C2p1.pdf

Nucleotide sequence of a portion of this resource.

Primer: EF1a-C_F RDB13974_A6C2a.seq
>D03172A2_A6C2_1_EF1a_C_F_E11_14.ab1
    1 TATGGGATTG CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGTCGAGAT GCCCTCTGAA
  121 TCTTTCTGTT TGGCTGCCCA GGCTCGCCTC GACTCCAAAT GGTTGAAAAC AGATATACAG
  181 CTTGCATTCA CAAGAGATGG GCTCTGTGGT CTGTGGAATG AAATGGTTAA AGATGGAGAA
  241 ATTGTATACA CTGGAACAGA ATCAACCCAG AACGGAGAGC TCCCTCCTAG AAAAGATGAT
  301 AGTGTCGAAC CAAGTGGAAC AAAGAAAGAA GATCTGAATG ACAAAGAGAA AAAAGATGAA
  361 GAAGAAACTC CTGCACCTAT ATATAGGGCC AAGTCAATTC TGGACAGCTG GGTATGGGGC
  421 AAGCAACCAG ATGTGAATGA ACTGAAGGAG TGTCTTTCTG TGCTGGTTAA AGAGCAGCAG
  481 GCCCTGGCCG TCCAGTCAGC CACCACCACC CTCTCAGCCC TGCGACTCAA GCAGAGGCTG
  541 GTGATCTTGG AGCGCTATTT CATTGCCTTG AATAGAACCG TTTTTCAGGA GAATGTCAAA
  601 GTTAAGTGGA AAAGCAGCGG TATTTCTCTG CCTCCTGTGG ACAAAAAAAG TTCCCGGCCT
  661 GCGGGCAAAG GTGTGGAGGG GCTCGCCAGA GTGGGATCCC GAGCGGCGCT GTCTTTTGCC
  721 TTTGCCTTCC TGCGCAGGGC CTGGCGATCA GGCGAGGATG CGGACCTCTG CAGTGAGCTG
  781 TTGCAGGAGT CCCTGGACGC CCTGCGAGCA CTTCCCGAGG CCTCGCTCTT TGACGAGAGC
  841 ACCGTGTCCT CTGTGTGGCT GGAGGTGGTG GAGAGAGCGA CCAGGTTTCC TCAGGTCCGT
  901 CGTGACGGGG GGATGTTCAC GGAACGCCAG CCACCAAAGG GCCAGGAAGC ATCCCCCCTG
  961 CAGACCAGCA CTTGGCCCTG GCCCATCCTT GCTGGGAGCT GGCTGTGCAG AGGAGGCACG
 1021 CTTGAGCCCA AATGTTTGTC TGCCCATTCC TTGTTTGTTT GCTTTCAGCT GTGGGGACAG
1081 CGGGGCCACA GGAGACCTGG ACTATGAAGC GATTCCGCCC CAGGGGCAAC CAAGT
//
Primer: Poly(A) region primer #0 RDB13974_A6C2b.seq
>D03172A2_A6C2_1_GNPmixture_D01_10.ab1
    1 AGGAAAAATT CGGTGAAAAT TCAGTGTACA ATGTGACAGT GGAGGGGACA CTGGTGAGTG
   61 GGGGGTGAAG AGGTGGAGGG TGACAGTGGA GCTTCCTTTC ACACACAGGC CTGACATGAT
  121 TCCTCCTGTC TGCTCCCTCC CACATCCCCA GTCCCCGTCC AGCAAGGCAA ATGTCCAGGC
  181 ACCTGCCTGG CCCTAAAAAG AGTCACAGTC CTGACCCTCG TGGGGGCAGG GCAGGTGTCC
  241 CGAGGCCACC CAGAAAAACA GGAGACACGT CGGTGATGTT CGGGAGTCAA ACCATGTCCC
  301 AAAAGTCTTA AGAAGAAAAA CGAGATTCCC CAGCAAATTC GTGGGACCCC AGGGCCATGC
  361 CTCCCAGCTC TGGCCACCTC TGCTTGTCCA GGGGCCTCAC AGCCAAGGGG ATGCAGGGGC
  421 CGCTGTGCAG TCGGCTGAGG CCAGAGTGAG GGGTGCAAGG ACCCAGATGG GGAACACTGC
  481 TGTTTAAATA TTAAACAGGG ATTTCTTGTC TCCCACGTGG GAGACCTGAT ACTCTCAAGG
  541 GTCCCAGGTG GGGACCCTCA CTCTAGGCTT TACTTGTACA GGGCCGCTTA GTGTCCTGTT
  601 AAATAATCTT GTGTAGAGTC CGAAGCAAAG GAGTCGACAT CCTCGTTATC TGAATCGTCG
  661 CTGCTGTCGT CGGCGGCTGG CTCTCCTGTA AGTGCGATGC GAGCGTAGTC ATCTGTGTCT
  721 ATGGACTTGC AGAAGTGGAT GGCGTACTTG AGCTTCTCCT CCAGCACCTG CTTGCAGGAA
  781 TACCTGGGCA GCTTTCAGCA AGAAGAAACA GGTGTAGGAC TCAGGGAGGA AGTGGGTCTG
  841 GAGGGTTGTA TTTATCCAAA CACCTGGATG ACGAAAGTCT TCGGCCCCGG AAGTCGCCGA
  901 TGGGTCCTGG GCTACCCTCG TCCGGCCCCA GAACGAAAGC AAAAGAAAAA GAGAACGCTC
  961 TGTTGTTTGG AAAAAGGACA CCAATCACCT TCCCAAAAAT CAACTTGGAT TCAGCGGATT
 1021 GACGGAAAGG GCTTCGAATG GCCTTTTTAA TAGGTGGATC AACCCGAAAC TTTTGAAGAA
1081 AAGTAGCTAG ACCGGA
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

original - reference Imai, Y., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680.

2016.07.26

GNP_filter3_RDBDEP_html_160610.pl