Resource data sheet
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pEF-LRRK2-FLAG-His

Expression vector of FLAG-His-tagged human LRRK2.

Catalog number RDB13973
Resource name pEF-LRRK2-FLAG-His
Clone info. Expression vector of FLAG-His-tagged human LRRK2.

[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB13973]
Vector backbone pEF-BOS
Size of vector backbone 5.3 kb
Selectable markers Amp^r
Classification Plasmid
Type_of_insert fragment human cDNA ()
Other clones with this gene 120892 | (indicated in NCBI Gene ID)
Deposotor's page Imai, Yuzuru
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)
(In Japanese [open/close])
Fee (non-profit org.)

We verified.

Data are summerized on test sheet below.

Test sheet RDB13973_A6C2p1.pdf

Nucleotide sequence of a portion of this resource.

Primer: EF1a-C_F RDB13973_A6C2a.seq
>D03172A1_A6C2_2_EF1a_C_F_G01_19.ab1
    1 ACTTTGGGAA TTGGCCCTTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG
   61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG AATTCTCTAG TCGAGATGGC
  121 TAGTGGCAGC TGTCAGGGGT GCGAAGAGGA CGAGGAAACT CTGAAGAAGT TGATAGTCAG
  181 GCTGAACAAT GTCCAGGAAG GAAAACAGAT AGAAACGCTG GTCCAAATCC TGGAGGATCT
  241 GCTGGTGTTC ACGTACTCCG AGCACGCCTC CAAGTTATTT CAAGGCAAAA ATATCCATGT
  301 GCCTCTGTTG ATCGTCTTGG ACTCCTATAT GAGAGTCGCG AGTGTGCAGC AGGTGGGTTG
  361 GTCACTTCTG TGCAAATTAA TAGAAGTCTG TCCAGGTACA ATGCAAAGCT TAATGGGACC
  421 CCAGGATGTT GGAAATGATT GGGAAGTCCT TGGTGTTCAC CAATTGATTC TTAAAATGCT
  481 AACAGTTCAT AATGCCAGTG TAAACTTGTC AGTGATTGGA CTGAAGACCT TAGATCTCCT
  541 CCTAACTTCA GGTAAAATCA CCTTGCTGAT ATTGGATGAA GAAAGTGATA TTTTCATGTT
  601 AATTTTTGAT GCCATGCACT CATTTCCAGC CAATGATGAA GTCCAGAAAC TTGGATGCAA
  661 AGCTTTACAT GTGCTGTTTG AGAGAGTCTC AGAGGAGCAA CTGACTGAAT TTGTTGAGAA
  721 CAAAGATTAT ATGATATTGT TAAGTGCGTT AACAAATTTT AAAGATGAAG AGGAAATTGT
  781 GCTTCATGTG CTGCATTGTT TACATTCCCT AGCGATTCCT TGCAATAATG TGGAAGTCCT
  841 CATGAGTGGC AATGTCAGGT GTTATAATAT TGTGGTGGAA GCTATGAAAG CATTCCCTAT
  901 GAGTGAAGAA ATTCAAGAAG TGAGTTGCTG TTTGCTCCAT AGGCTTACAT TAGGTAATTT
  961 TTTCAATATC CCTGTATTTA ACCGAAGTCC ATGAGTTTGT GGTGAAAGCT GGTGCAGCAG
 1021 TACCCAGAGA ATGCCAGCAA TTGCAGAATC TCAGCCGCTC AAGCTGTTTG GTCCTTCTTA
1081 ATCCAAGGAT TTTAGAGGC
//
Primer: Poly(A) region primer #0 RDB13973_A6C2b.seq
>D03172A1_A6C2_2_GNPmixture_B01_04.ab1
    1 AGGAAAAATC GGTGAAGCTC AGCGTACAAT GTGGCAGTGG AGGGGACACT GGTGAGTGGG
   61 GGGTGAAGAG GTGGAGGGTG ACAGTGGAGC TTCCTTTCAC ACACAGGCCT GACATGATTC
  121 CTCCTGTCTG CTCCCTCCCA CATCCCCAGT CCCCGTCCAG CAAGGCAAAT GTCCAGGCAC
  181 CTGCCTGGCC CTAAAAAGAG TCACAGTCCT GACCCTCGTG GGGGCAGGGC AGGTGTCCCG
  241 AGGCCACCCA GAAAAACAGG AGACACGTCG GTGATGTTCG GGAGTCAAAC CATGTCCCAA
  301 AAGTCTTAAG AAGAAAAACG AGATTCCCCA GCAAATTCGT GGGACCCCAG GGCCATGCCT
  361 CCCAGCTCTG GCCACCTCTG CTTGTCCAGG GGCCTCACAG CCAAGGGGAT GCAGGGGCCG
  421 CTGTGCAGTC GGCTGAGGCC AGAGTGAGGG GTGCAAGGAC CCAGATGGGG AACACTGCTG
  481 TTTAAATATT AAACAGGGAT TTCTTGTCTC CCACGTGGGA GACCTGATAC TCTCAAGGGT
  541 CCCAGGTGGG GACCCTCACT CTAGGCTTTA CTTGTACAGC GGCTAGCATT TAGGTGACAC
  601 TATAGAATAG GGCCCTCTAG AGCAGCCAAC TCAGCTTCCT TTCGGGCTTT GTTAGCAGCC
  661 GGATCTCAGT GGTGGTGGTG GTGGTGCTCG AAGATCTTGT CATCGTCGTC CTTGTAGTCC
  721 TCGACCTCGA CGCGGCCGCA CTCAACAGAT GTTCGTCTCA TTTTTTCAGC TAATTCTTTT
  781 CTCACTTCAA TGTGTTTTTC TAAATTTTGC ACTTCATGTG GAAGATTGAT GTCCCAAAAC
  841 GGTCAAGCAA GATTGTATCT CTTTCTGCTT TTGTGTACCT TCAGTATTTT TCCGGTTGTA
  901 GCCCCAATAC CAAGCATGAC ATTTTTTAAA GGCTTCCCTA AGCTGTGCCT GTCCATCCAA
  961 TGACCTCTTG ACCCGAAATT ACCAAAAGGT TGTAAAATTA ACCACCGTTA ATAAAGTCCG
 1021 ACCGAAGTTG AAAGATCCCA AGGGAGGTAA AAATATGGGC CCCTCCCTTC CCAGATTCCT
1081 AATCCCCTAG AAGCCAGGTG TTTCCTTTTC CTGACAGAGT CACCCTGAT
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

original - reference Imai, Y., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680.

2016.07.26

GNP_filter3_RDBDEP_html_160610.pl