Resource data sheet
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pEF/RTAdelta/myc/ER

Expression clone of Ricin A chain nontoxic mutant (RTAdelta).

Catalog number RDB13411
Resource name pEF/RTAdelta/myc/ER
Clone info. Expression clone of Ricin A chain nontoxic mutant (RTAdelta).
RTAdelata: ricin nontoxic allele that lacks the critical residues for its toxicity of ricin A chain.
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB13411]
Vector backbone pEF/myc/ER
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Classification Plasmid
Type_of_insert fragment Ricinus communis ()
Deposotor's page Tadashi Suzuki
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions for distribution Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Proc. Natl. Acad. Sci. U.S.A., 112 (5): 1398-1403 (2015))
(In Japanese [open/close])
Remarks ((Additional form)) Approval Form(FormD)
(In Japanese [open/close])
Fee (non-profit org.)

This clone will be sequenced a portion and digested by restriction enzyme for verification.

References and tips

original - reference Huang, C., Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Proc. Natl. Acad. Sci. U.S.A. 112 (5): 1398-1403 (2015). PMID 25605922.

2016.07.26

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