Resource data sheet


Expression vector of A. oryzae tannase in P. pastoris.

Catalog number RDB13297
Resource name pPICZalphaC_AotanA
Clone info. Expression vector of A. oryzae tannase in P. pastoris.
Expression was confirmed by the Depositor with SDS-PAGE and enzyme assays.
[Data sheet:]
Vector backbone pPICZalphaC
Size of vector backbone 3.3 kb
Selectable markers Zeo^r (E. coli, Pichia pastoris)
Classification Plasmid
Type_of_insert fragment A. oryzae genomic DNA ()
Deposotor's page Takuya KOSEKI
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J. Biosci. Bioeng., 118(4), 392-395 (2014))
(In Japanese [open/close])
Fee (non-profit org.)

This clone will be sequenced a portion and digested by restriction enzyme for verification.

References and tips

original - reference Mizuno, T., Biochemical characterization of Aspergillus oryzae native tannase and the recombinant enzyme expressed in Pichia pastoris. J. Biosci. Bioeng. 118 (4): 392-395 (2014).original PMID 24856589.