Resource data sheet


HCV IRES-dependent expression vector of 2A-2B

Catalog number RDB13261
Resource name pUC-T7-HCV HA-2A-2B-FLAG
Clone info. HCV IRES-dependent expression vector of 2A-2B.
Encephalomyocarditis virus (EMCV), GenBankAcc. No: M81861. Expression was confirmed by depositor with Western blot.
[Data sheet:]
Vector backbone pUC-T7-HCV-HA
Size of vector backbone 3.4 kb
Selectable markers Amp^r
Classification Plasmid
Type_of_insert fragment encephalomyocarditis virus cDNA ()
Deposotor's page Imataka, Hiroaki
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)
(In Japanese [open/close])
Fee (non-profit org.)

This clone will be sequenced a portion and digested by restriction enzyme for verification.

References and tips

original - reference Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.