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pUC-T7-HCV HA-2A-2B-FLAG

HCV IRES-dependent expression vector of 2A-2B

Catalog number RDB13261
Resource name pUC-T7-HCV HA-2A-2B-FLAG
Clone info. HCV IRES-dependent expression vector of 2A-2B.
Encephalomyocarditis virus (EMCV), GenBankAcc. No: M81861. Expression was confirmed by depositor with Western blot.
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB13261]
Vector backbone pUC-T7-HCV-HA (Plasmid)
Size of vector backbone 3.4 kb
Selectable markers Amp^r
Gene/insert name encephalomyocarditis virus 2A, 2B cDNA
Deposotor's page Imataka, Hiroaki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13261 pUC-T7-HCV HA-2A-2B-FLAG DNA solution

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

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Reference

original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.
others Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009).

2017.04.28

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