HCV IRES-dependent expression vector of 2A-2B
|Resource name||pUC-T7-HCV HA-2A-2B-FLAG|
|Clone info.||HCV IRES-dependent expression vector of 2A-2B.
Encephalomyocarditis virus (EMCV), GenBankAcc. No: M81861. Expression was confirmed by depositor with Western blot.
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB13261]
|Vector backbone||pUC-T7-HCV-HA (Plasmid)|
|Size of vector backbone||3.4 kb|
|Gene/insert name||encephalomyocarditis virus 2A, 2B cDNA|
|Deposotor's page||Imataka, Hiroaki ||
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
|Terms and conditions set forth by the DEPOSITOR||In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)|
(Japanese text [open/close])
利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Biol Chem. 289(46): 31960-71, 2014)。
|Catalog #||Resource name||Shipping form||Fee (non-profit org.)|
|RDB13261||pUC-T7-HCV HA-2A-2B-FLAG||DNA solution|
This clone will be sequenced a portion and digested by restriction enzyme for examination.
|original||Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.|
|others||Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009).|