Resource data sheet
Prev.

pROSA-floxNeo-Fucci2a

Fucci2a cell cycle reporter. Targeting vector for R26 locus

Catalog number RDB13081
Resource name pROSA-floxNeo-Fucci2a
Clone info. Fucci2a cell cycle reporter composed of Fucci2, mVenus-hGem(1/110) and mCherry-hCdt1(30/120). Targeting vector for R26 locus. Fucci2aR ROSA26 knock-in mice (cat.# RBRC06511) is available from the Experimental Animal Division.
Vector backbone pCAGiP (Plasmid)
Selectable markers Amp^r
Gene/insert name Human Cdt1 cDNA Human Gem cDNA Mouse ROSA 26 genomic DNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Depositor Mort, Richard |
 
Other clones in our bank human CDT1 (NCBI Gene 81620) | human GMNN (NCBI Gene 51053) | mouse Gt(ROSA)26Sor (NCBI Gene 14910) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature(s) designated by the DEPOSITOR is requested. Cell Cycle 13:17, 2681-2696, 2014. 3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 5) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
(Japanese text [open/close])
Remarks ((Remarks)) Please contact us. We will provide you with appropriate documents.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13081 pROSA-floxNeo-Fucci2a DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13081_A5Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB13081_A5Aja.seq
>13081_13081_A5Aj_pAxCA_F1_8_B10_04.ab1
    1 CGTCCTGCTA CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT
   61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCGATATC AAGCTTATCG AGCGGCCGCT
  121 CTAGAACTAG TGGATCCATA ACTTCGTATA GCATACATTA TACGAAGTTA TCCCAGATCC
  181 CCCGGGCTGC AGCCAATATG GGATCGGCCA TTGAACAAGA TGGATTGCAC GCAGGTTCTC
  241 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT
  301 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG
  361 ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA
  421 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC
  481 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA
  541 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC
  601 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC
  661 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG
  721 CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT
  781 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC
  841 TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC
  901 TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC
  961 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG
 1021 AAATGACCGA CCCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTT CCACCGCGCG
 1081 TTCTATGAAA GATGGGGCCT TCGTATCGGT TTCAGGAACG ATGCCTGGAT GATTCCCTCC
1141 AGCCGGCGGG GATTCTCTCT CAAG
//
Primer: pAxCALNL_F1 RDB13081_A5Ajb.seq
>13081_13081_A5Aj_pAxCALNL_F1_8_B01_04.ab1
    1 ATTATGTATC TTATCATGTC TGGATCTCCC GATATCCCCA TAACTTCGTA TAGCATACAT
   61 TATACGAAGT TATGGATCCC CCGGGCTGCA GGAATTCCTC GACACGCGTA TGGTGAGCAA
  121 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA
  181 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA
  241 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA
  301 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT
  361 CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT
  421 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT
  481 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA
  541 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG
  601 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC
  661 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT
  721 GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG
  781 CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC
  841 GCTCGAGCCC AGCCCCGCCA GGCCCGCACT CCGCGCCCCG GCCTCCGCTA CCAGTGGCAG
  901 CCGCAAGCGC GCCCGCCCGC CCGCCGCCCC CGGACGCGAC CAGGCCAGGC CACCGGCCCG
  961 CAGAGACTGC GGCTGTCGGT GGACGAGGTT TCCAGCCCCA GTACCCCCCG AGGCCCCAGA
 1021 CATCCCAGCC TGCCCCTTCT CCGGGCCAGA GATAAAGAAT CCACCCCCGG CAGCAGTCAG
 1081 CGCTCCCACC TTGAACATCC GCGCAGACAG ACATCAATTC ATGAGGTCGT GCCTTCCTCC
1141 TTCTTTTCAC CTGTATG
//
Primer: PGK_N_rev RDB13081_A5Ajc.seq
>13081_13081_A5Aj_PGK_N_R_F02_17.ab1
    1 GAGGGGGGGG GATTGGACGC TCCCTACCGG GAGAATTCGA TATCAAGCTT ATCGATACGT
   61 CGACGGTATC GATAAGCTAG ATGTAAAGAA ATATGTTCTA TCAGAACAAG GTAGTATAAA
  121 GCTGGTAGGT ATACAAAACG CTAGACTAGT TTCTATCCCT GACCCTTAAT CTGCTAGTAT
  181 ATCCGTAGGA AGTTGCTTAA GTGCCACTAG TACCAACAGC CTCTGTTAGA GTGAACAGTA
  241 GATTTTAATG AAGGGCCAAT AAAGACCACT TAAGACTACT GACTGAATTA AAAATCCACC
  301 CAGTGGAGTT AGTATTGGTT ATAGTTATAT AACAAAATAT CCAACTTAGC CAGGCGTGGT
  361 GGTGCACGCC TTTAATCCCG GCACTCAGGA GGCAGAGGCA GGCGGATTTC TGAGTTCGAG
  421 GCCAGCCTGG TCTACAAAGT GAGTTCCAGG ACAGCCAGGG CTATACAGAG AAACCCTGTC
  481 TCGAAAAACC AAAAAAAAAA AAAAAAAGGA AAAAAAATAT GGCTTGAAAA CATGCTATTG
  541 TTGTCAAAAG GAGATCCAAA CAAGTATTTC TTTGAACAAA AATTATGTAG AAAACAGGGT
  601 GATAAAGAAC AAGGGCTTTT TAAAAAAGGC CAGCATTATT TGAGATATAC AAGTGACTCC
  661 AATTCAAGCC TAAAGGCCAC TCAATGCTCA CTAACAGTGC TAATCAGAAT CAATTCCTTA
  721 GCCCAGTCAA AAATAGTCCT GAATATCTTC TGGATAAACA TGTCCTCCAA CATATAAACT
  781 AGTATACACT AACAAAACGT CTCAACTTCA AGGTGAAATG CTTGACTCCT AGACTTGTGA
  841 CCCAGCAAAG TGCTCTATAG GTAGGGTTAC TAGGTCAGAG AGTCTTGCCT GCAAACCACA
  901 ATTATACAAT TCCACCAAAT GTAAGACATG TAAAAAATGA CTTATTTAGC TCAGGTTTTG
  961 AAAATAAAAA ATCAAACCCC ACAAAGACCT AAACTTTCAC ATTAAGTACA AATGTTTAAC
 1021 ATATATAAAC ATGTTTTTGC CCTTGATATA TGAAATCATA ATGACATCAC CCTTGACAAG
 1081 GAAACCTTAA AGTTTAATTT GAACCAACCA TTCAGCCTAG GTAAGCCTAG CACCATGGAT
1141 TCCAGGATAG TTGCCAAGGG GCAAAACCTC CCAAAG
//
Primer: M13_-40 RDB13081_A5Ajd.seq
>13081_13081_A5Aj_M13(-40)_D03_12.ab1
    1 CTTCCAACTA CGGCGCGTAA TACGACTCAC TATAGGGCGA ATGTGGAGCG TCCACCGCGG
   61 CAGGCCCTCC GAGCGTGGTG GAGCCGTTCT GTGAGAGAGC CGGGTACGAG TCGTGACGCT
  121 GGAAGGGGCA AGCGGGTGGT GGGCAGGAAT GCGGTCCGCC CTGCAGCAAC CGGAGGGGGA
  181 GGGAGAAGGG AGCGGAAAAG TCTCCACCGG ACGCGGCCAT GGCTCGGGGG GGGGGGGGCA
  241 GCGGAGGAGC GCTTCCGGCC GACGTCTCGT CGCTGATTGG CTTCTTTTCC TCCCGCCGTG
  301 TGTGAAAACA CAAATGGCGT GTTTTGGTTG GCGTAAGGCG CCTGTCAGTT AACGGCAGCC
  361 GGAGTGCGCA GCCGCCGGCA GCCTCGCTCT GCCCACTGGG TGGGGCGGGA GGTAGGTGGG
  421 GTGAGGCGAG CTGGACGTGC GGGCGCGGTC GGCCTCTGGC GGGGCGGGGG AGGGGAGGGA
  481 GGGTCAGCGA AAGTAGCTCG CGCGCGAGCG GCCGCCCACC CTCCCCTTCC TCTGGGGGAG
  541 TCGTTTTACC CGCCGCCGGC CGGGCCTCGT CGTCTGATTG GCTCTCGGGG CCCAGAAAAC
  601 TGGCCCTTGC CATTGGCTCG TGTTCGTGCA AGTTGAGTCC ATCCGCCGGC CAGCGGGGGC
  661 GGCGAGGAGG CGCTCCCAGG TTCCGGCCCT CCCCTCGGCC CCGCGCCGCA GAGTCTGGCC
  721 CGCGCGCCCC TGCGCAACGG TGGCAAGAAG CGCGCGCTGG GGGGGCGGGG ACGGGCAGTA
  781 GGGGCTGAAG CGGCTGCGGG GGCGGGTGCA AAGCACGTTT TCCGGACTTG AGTTGGCCTC
  841 AAGAGGGGGC GGTGCTTGAG CCCAGACCTC CATCGCGCAC TCCGGGGAGT GGAGGGGAAG
  901 GGAGCGAAGG GCTCATTTGG GCTTGTTTTT GGAGGGCAGG AAAGCACTTT GCCTCGTCCC
  961 AAAGTCGCCT CCTGGAGTTT GTTTATACAG TTAAGGGGAG ACTGGCAAGT GGGGAGTAAG
 1021 GCGCGCGGGA AGAAAAGAGC CGCACCACGT TCTTCCCGAG AAGGGGGGGA GGGGGCGAAC
1081 GGGTGT
//
Primer: CMV_en_R RDB13081_A5Aje.seq
>13081_13081_A5Aj_CMV_en_R_8_A10_01.ab1
    1 GTCTTTATTG ATTACTATTA ATAACTAGTC AATAATCAAT GTCGCACTTA ATTAAACGCG
   61 CCGGCTAGAA GATGGGCGGG AGTCTTCTGG GCAGGCTTAA AGGCTAACCT GGTGTGTGGG
  121 CGTTGTCCTG CAGGGGAATT GAACAGGTGT AAAATTGGAG GGACAAGACT TCCCACAGAT
  181 TTTCGGTTTT GTCGGGAAGT TTTTTAATAG GGGCAAATAA GGAAAATGGG AGGATAGGTA
  241 GTCATCTGGG GTTTTATGCA GCAAAACTAC AGGTTATTAT TGCTTGTGAT CCGCCTCGGA
  301 GTATTTTCCA TCGAGGTAGA TTAAAGACAT GCTCACCCGA GTTTTATACT CTCCTGCTTG
  361 AGATCCTTAC TACAGTATGA AATTACAGTG TCGCGAGTTA GACTATGTAA GCAGAATTTT
  421 AATCATTTTT AAAGAGCCCA GTACTTCATA TCCATTTCTC CCGCTCCTTC TGCAGCCTTA
  481 TCAAAAGGTA TTTTAGAACA CTCATTTTAG CCCCATTTTC ATTTATTATA CTGGCTTATC
  541 CAACCCCTAG ACAGAGCATT GGCATTTTCC CTTTCCTGAT CTTAGAAGTC TGATGACTCA
  601 TGAAACCAGA CAGATTAGTT ACATACACCA CAAATCGAGG CTGTAGCTGG GGCCTCAACA
  661 CTGCAGTTCT TTTATAACTC CTTAGTACAC TTTTTGTTGA TCCTTTGCCT TGATCCTTAA
  721 TTTTCAGTGT CTATCACCTC TCCCGTCAGG TGGTGTTCCA CATTTGGGCC TATTCTCAGT
  781 CCAGGGAGTT TTACAACAAT AGATGTATTG AGAATCCAAC CTAAAGCTTA ACTTTCCACT
  841 CCCATGAATG CCTCTCTCCT TTTTCTCCAT TTATAAACTG AGCTATTAAC CATTAATGGT
  901 TTCCAGGTGG ATGTCTCCTC CCCCAATATT ACCTGATGTA TCTTACATAT TGCCAGGCTG
  961 ATATTTTAAA GACATTAAAA GGTATATTTC ATTATTGAGC CACATGGGTA TTGATTACTG
 1021 CTTTACTAAA ATTTTGTCAT TGTACACATC TGTAAAAGGT GGTTTCCCTT TTGGGAATGC
 1081 AAAGTTCAGG TGATTGATGT CTTTCTGACC TTACGTCTGT GAGCCTGCAT TTTTTTCTAA
1141 TTTAAGCAGG GTGCTTTTCT T
//
Primer: EGFP-N RDB13081_A5Ajf.seq
>13081_13081_A5Aj_EGFP_N_8_A01_01.ab1
    1 GGCCCGGCGA CGCTCCTCGC CCTTGCTCAC CATGGGTCCA GGATTCTCTT CGACATCGCC
   61 ACAAGTGAGA AGAGAGCCAC GGCCTTCCAA TTGGATGGTG TCCTGGTCCT GCGCGGATGT
  121 CAGGTGGGGC GGCTGACCTG CTGCCGGGGT GGATTTCTTT ATCTTCTGGC CCGGAGAAGG
  181 GCAGGCTGGG ATGTCTGGGG CCTCGGGGGT ACTGGGGCTG GAAACCTCGT CCACCGACAG
  241 CCGCAGTCTC CTGCGGGCCG GTGGCCTGGC CTGGTCGCGT CCGGGGGCGG CGGGCGGGCG
  301 GGCGCGCTTG CGGCTGCCAC TGGTAGCGGA GGCCGGGGCG CGGAGTGCGG GCCTGGCGGG
  361 GCTGGGCTCG AGCGGCCGCC AGTGTGATGG ATATCCCTTG TACAGCTCGT CCATGCCGCC
  421 GGTGGAGTGG CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG
  481 GGAGGTGATG TCCAACTTGA TGTTGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT
  541 GGCCTTGTAG GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT
  601 CTGCTTGATC TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCGG AGGAGGCCTC
  661 CCAGCCCATG GTCTTCTTCT GCATTACGGG GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG
  721 CTTCACCTTG TAGATGAACT CGCCGTCCTG CAGGGAGGAG TCCTGGGTCA CGGTCACCAC
  781 GCCGCCGTCC TCGAAGTTCA TCACGCGCTC CCACTTGAAG CCCTCGGGGA AGGACAGCTT
  841 CAAGTAGTCG GGGGATGTCG GCGGGGTGCT TCACGTAGGC CTTGGAGCCG TACATGAACT
  901 GAGGGGACAG GATGTCCCAG GCGAAAGGGC AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG
  961 GCGGTCTGGG TGCCCTCGTA GGGCGGCCCT CGCCCTCGCC CTCGATCTCG AACTCGTGGC
 1021 CGTTCACGGG AGCCCTCCCA TGTGCACCTT TGAGCGCATG AACTCATGAA TGATGGCATG
 1081 ATATCTCCTC GCCTGCTCAC ATACGCGTGT CGAGAGTTCC CTGCCAGCCC GGGGGGAATC
1141 CAA
//
Primer: EGFP-C RDB13081_A5Ajg.seq
>13081_13081_A5Aj_EGFP_C_8_D01_10.ab1
    1 GCGTACTCTC GGCATGGACG AGCTGTACAA GGGATATCCA TCACACTGGC GGCCGCTCGA
   61 GATGAATCCC AGTATGAAGC AGAAACAAGA AGAAATCAAA GAGAATATAA AGAATAGTTC
  121 TGTCCCAAGA AGAACTCTGA AGATGATTCA GCCTTCTGCA TCTGGATCTC TTGTTGGAAG
  181 AGAAAATGAG CTGTCCGCAG GCTTGTCCAA AAGGAAACAT CGGAATGACC ACTTAACATC
  241 TACAACTTCC AGCCCTGGGG TTATTGTCCC AGAATCTAGT GAAAATAAAA ATCTTGGAGG
  301 AGTCACCCAG GAGTCATTTG ATCTTATGAT TAAAGAAAAT CCATCCTCTC AGTATTGGAA
  361 GGAAGTGGCA GAAAAACGGA GAAAGGCGCT GTAAGGTACC ACATGTGGCG CGCGCGCCTT
  421 CGATGATCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT
  481 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC
  541 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT
  601 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT
  661 GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCTGCA GCCCAAGCTT GGGCTGCAGG
  721 GCGCGCCCGA TTAAGGCTAG AAAGACTGGA GTTGCAGATC ACGAGGGAAG AGGGGGAAGG
  781 GATTCTCCCA GGCCCAGGGC GGTCCTCAGA AGCCAGGAGG CAGCAGAGAA CTCCCAGAAA
  841 GGTATTGCAA CACTCCCCTC CCCCCTCCGG AGAAGGGTGC GGCCTTCTCC CCGCCTACTC
  901 CACTGCAGCT CCCTTACTGA TAACAACTCA GAGCGACTTT GGGAGAGCAA GTGCTTCCTG
  961 CCTCCAAAAC AGCCCAACTG AGCCCTCGCT CCTTTCCTCC ACTCCCCGGA GTGCGCGATG
 1021 GGAGGTCTGG CTCAGCACGC CCCTCTTGAG GCAACTCAAG TCGGGAAACG TGCTGCACCC
 1081 GCCCCGCAGC GCTCAGCCTT ACTGACGTTC CGCCCCAGCG CGCGCATCTG ACCACGTTGC
1141 CGCCAAGGGG GACGTC
//
Primer: T3 RDB13081_A5Ajh.seq
>13081_13081_A5Aj_T3_8_C01_07.ab1
    1 ATGTCGGGAC CCCCTCGAGC CCCAGCTGGT TCTTTCCGCC TCAGAAGCCA TAGAGCCCAC
   61 CGCATCCCCA GCATGCCTGC TATTGTCTTC CCAATCCTCC CCCTTGCTGT CCTGCCCCAC
  121 CCCACCCCCC AGAATAGAAT GACACCTACT CAGACAATGC GATGCAATTT CCTCATTTTA
  181 TTAGGAAAGG ACAGTGGGAG TGGCACCTTC CAGGGTCAAG GAAGGCACGG GGGAGGGGCA
  241 AACAACAGAT GGCTGGCAAC TAGAAGGCAC AGTCGAGGCT GATCAGCGAG CTCTAGGATC
  301 TGCATTCCAC CACTGCTCCC ATTCATCAGT TCCATAGGTT GGAATCTAAA ATACACAAAC
  361 AATTAGAATC AGTAGTTTAA CACATTATAC ACTTAAAAAT TTTATATTTA CCTTAGAGCT
  421 TTAAATCTCT GTAGGTAGTT TGTCCAATTA TGTCACACCA CAGAAGTAAG GTTCCTTCAC
  481 AAAGAGATCG CCTGACACGA TTTCCTGCAC AGGCTTGAGC CATATACTCA TACATCGCAT
  541 CTTGGCCACG TTTTCCACGG GTTTCAAAAT TAATCTCAAG TTCTACGCTT AACGCTTTCG
  601 CCTGTTCCCA GTTATTAATA TATTCAACGC TAGAACTCCC CTCAGCGAAG GGAAGGCTGA
  661 GCACTACACG CGAAGCACCA TCACCGAACC TTTTGATAAA CTCTTCCGTT CCGACTTGCT
  721 CCATCAACGG TTCAGTGAGA CTTAAACCTA ACTCTTTCTT AATAGTTTCG GCATTATCCA
  781 CTTTTAGTGC GAGAACCTTC GTCAGTCCTG GATACGTCAC TTTGACCACG CCTCCAGCTT
  841 TTCCAGAGAG CGGGTTTTCA TTATCTACAG AGTATCCCGC AGCGTCGTAT TTATTGTCGG
  901 TACTATAAAA CCCTTTCCAA TCATCGTCAT AATTTCCTTG TGTACCAGAT TTTGGCTTTT
  961 GTATACCTTT TTTGAATGGA AATCTACATA ACCAGGGTTT AGTCCCGTGG TACGAAGAAA
 1021 AGTTTTCCAT CACAAAAGAT TTAGAGATCA CACATCATCA GGATCATGCG AGACTGCAGT
 1081 CGAAGGACAG AGATGAGGAA GAGAGAACAG CGCGCCAGGA CGTGCGCCTT TTTGAAGCGC
1141 GGGTGCA
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content G1 cdks and cdk inhibitors (English text)
Featured content G1-S trasition (English text)
Featured content p53 dependent and independent G1 arrest (English text)
Featured content Summary of G2/M transition (English text)
Featured content p53 dependent G2 arrest (English text)
Featured content DNA damage (English text)
Featured content Rb pathway (English text)

References

original Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356.
reference Bertero, A., Fucci2a mouse upgrades live cell cycle imaging. Cell Cycle 14 (7): 948-949 (2015). PMID 25807308.
reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.
reference Glover, J.D., Maintenance of distinct melanocyte populations in the interfollicular epidermis. Pigment Cell Melanoma Res. 28 (4): 476-480 (2015). PMID 25847135.

2017.08.30

GNP_filter3_RDBDEP_html_170829.pl