Resource data sheet
Prev.

Histac-K12 pcDNA3.1

For the detection of acetylation at the 12th lysine residues of histone H4 by the BRD2 bromodomain.

Catalog number RDB12841 (link to web catalog)
Resource name Histac-K12 pcDNA3.1
Clone info. For the detection of acetylation at the 12th lysine residues of histone H4 by the BRD2 bromodomain.
Expression was confirmed by observation fluorescent in COS7 cells under the microscope by the depositor.
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB12841]
Vector backbone pcDNA3.1
Size of vector backbone 5.5kb
Classification Plasmid
Type_of_insert fragment Human cDNA ()
Other clones with this gene 6046 | 320332 | (indicated in NCBI Gene ID)
Deposotor's page Sasaki, Kazuki
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested(ChemBio. 18, 495-507, (2011)). The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
(In Japanese [open/close])
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally [Link]
(In Japanese [open/close])
Fee (non-profit org.)

We verified.

Data are summerized on test sheet below.

Test sheet RDB12841_A4Ih.pdf

Nucleotide sequence of a portion of this resource.

Primer: CMV-Forward RDB12841_A4Iha.seq
>RDB12841_3_CMV_Forward_D11_11.ab1
    1 ACCGTCAACT CCGCCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
  121 TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT TGGTACCATG
  181 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC
  241 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
  301 AAGCTGACCC TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
  361 GTGACCACCC TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG
  421 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC
  481 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG
  541 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG
  601 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCACCG CCGACAAGCA GAAGAACGGC
  661 ATCAAGGCCA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCGAC
  721 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
  781 CTGAGCTACC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG
  841 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGGAATTC
  901 ACCCCTGCCA ACCCACCACC CCCGGAGGTG TCCAATCCCA AAAAGCCAGG GACGAGTTAC
  961 CAACCAGCTG CAATACCTAC ACAAAGGTAG TGATGAAAGG CTCTGTGGAA ACATCAGTTC
 1021 GGCATGGGCC ATTCCCGGCA GCCCTGTGAA TGCTGTCAAA CTGGGTTCTT CCCGGATTAT
 1081 CCACAAAATT AATAAAAACA GCCTATGGAA CAATGGGTAC CTATTAAGAG AGACTGAAAA
1141 ACATTATTAT TGGGCTGCTT CAGAAGGTGT TAGTGCCAAG GAATTA
//
Primer: BGH_rev RDB12841_A4Ihb.seq
>RDB12841_3_BGH_rev_E11_14.ab1
    1 CCTTTACGGG ACTCTAGACT CGAGTTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC
   61 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT
  121 CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT
  181 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG
  241 GATCTTGAAG TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG
  301 GCTGATGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT
  361 GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT
  421 CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT
  481 GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA AGCACTGCAC
  541 GCCCCAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA
  601 GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT
  661 GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA
  721 CAGCTCCTCG CCCTTGCTCA CCATGATATC AACCACTTTG TACAAGAAAG CTGGGTCGAC
  781 GCCGCCGAAG CCGTACAGAG TGCGGCCCTG GCGCTTGAGC GCGTACACCA CGTCCATAGC
  841 CGTGACCGTC TTGCGCTTGG CGTGCTCGGT GTAGGTGACG GCGTCGCGGA TCACATTCTC
  901 AAGGAAAACC TTGAGGACAC CGCGAGTCTC CTCGTAGATG AGACCCGAGA TGCGCTTCAC
  961 GCCGCCACGC CGAGCCAACC GCCCGGATAG CGGGCTTGGG TAATGCCCTG ATGTTGTCGC
 1021 GGAGAACCTT GCGTGTCGCT TTGCGGCCAC CTTTCCCCAG ACCCCTTGCG CCTTTTCCTC
 1081 GTCCTGACAT GATCCAGATC TCACACTGAA CCAACACTTC AGGATCTCTT CAACCAGATC
1141 CACCTCCTCC GTTCGGACCG GCAGGTTAGG AG
//
Primer: SV40pro_F_V2 RDB12841_A4Ihc.seq
>RDB12841_3_SV40pro_F_V2_F11_17.ab1
    1 GGTCGAGCCT TTTTTTTGGG AGGCCTAGGC TTTTTGGCAA AAAAGCTCCC GGGAGCTTGT
   61 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA
  121 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG
  181 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC
  241 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA
  301 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC
  361 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA
  421 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT
  481 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA
  541 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG
  601 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC
  661 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT
  721 GGATTCATCG ACTGTGGCCG GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTTGG
  781 CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT
  841 ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT
  901 TCTGAGCGGG AACTCTGGGG GGTTCGAAAT TGACCGACCA AGCGAACGCC CAAACCTGCC
  961 ACCACGAGAT TTCGATTTCC ACCCGCCGCC CTTCTATGAA AGGGTGGGGC TTCGGAAATC
 1021 GTTTTTCCGG GACGCCGGGC TGGAATGATC CCTCCAGCGC GGGGATCTCA TGGCTGGGAG
 1081 GTTCCTTCGC CCACCCCCAA CCTCGTTTAT TGGCAGCCTA ATAATGGGTT AACAAATAAA
 1141 GGCCAATTAG CATCGAAAAT TCCACAATAA GGCAATTTTT TTCACACTGG GCAATTCCTA
1201 TAAGCGTCTC CG
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file Sequence RDB12841z.seq from Depositor
Electronic file PDF RDB12841.pdf from Depositor
Featured content Histac for visualization of acetylation of the Histon H4 (in English)
Featured content Epigenetics clone (in English)
original - reference Ito, T., Real-time imaging of histone H4K12-specific acetylation determines the modes of action of histone deacetylase and bromodomain inhibitors. Chem. Biol. 18 (4): 495-507 (2011). PMID 21513886.

2016.07.26

GNP_filter3_RDBDEP_html_160610.pl