Resource data sheet
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pDUAL-FFH1

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Catalog number RDB06185 (link to web catalog)
Resource name pDUAL-FFH1
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter.
Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB06185]
Vector backbone pDUAL
Selectable markers Amp^r
Classification Plasmid
Reference of nucleotide sequence AB364052.1 (DDBJ accession number)
Deposotor's page Yoshida, Minoru | Matsuyama, Akihisa |
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Fee (non-profit org.)

We examined.

Data are summerized on test sheet below.

Test sheet RDB14484_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14484_A6L7a.seq
>06185_14484_A6L7_nmt1Fw_H01_08_ABI08.ab1
    1 GAGAATTAAT TTAAATCAGG GGAAAAACGG TAACTCTCGG CTACTGGATG GTTCAGTCAC
   61 CCAACGATTA CTGGGGAGAG AAAACAGGGC AAAAGCAAAG CTTAAAGGAA TCCGATTGTC
  121 ATTCGGCAAT GTGCAGCGAA ACTAAAAACC GGATAATGGA CCTGTTAATC GAAACATTGA
  181 AGATATATAA AGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT
  241 TTCAATCTCA TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC
  301 CTGGATCCGG ATATCATGGA TTATAAGGAT GATGACGATA AACCCATGGA CTACAAGGAC
  361 GACGATGACA AACATCACCA CCATCACCAC TGAGATCTCG ACTCTAGAGG ATCCGGCGCG
  421 CCACTTCTAA ATAAGCGAAT TTCTTATGAT TTATGATTTT TATTATTAAA TAAGTTATAA
  481 AAAAAATAAG TGTATACAAA TTTTAAAGTG ACTCTTAGGT TTTAAAACGA AAATTCTTAT
  541 TCTTGAGTAA CTCTTTCCTG TAGGTCAGGT TGCTTTCTCA GGTATAGTAT GAGGTCGCTC
  601 TTATTGACCA CACCTCTACC GGCAGATCCG CTAGGGATAA CAGGGTAATA TACTAGTAAG
  661 ATGATTCTTT TATCGTCGAT AACGATGAGG ATGTAGACGA TTATGATGAG AGTGATTAGC
  721 TTCTAAAATT TGGTGTTTTC AAACGCTATT TACAGTACAA TCTGAAAAAA TTGGATGAAC
  781 ATTGTAAATG GTAGGATACG AGTTCCAATT AATGTATTTT TTTAAGTATT ACACTTAGCT
  841 TATTAATTTT ATATATACAT TCTACCTCTG TGGGTTTAAA GACACTCGTA CACATACTCA
  901 ATTATTTATG ACATTATAAT GCATATTCGT ACTGTCTATT AAACATTTTT TTTTGACATT
  961 AAAAATTTCG TTAACTACGT AGAAGCGCTG ATATGAGCTA CTATTTTAAT TATCTAGAAA
 1021 CAAGATCATT ATTATTAAGG TAAGCTTCAA GGATACTTGT AATTTCGTAA AGGCCAAGTC
1081 CAGGCCGAG
//
Primer: LEU1pro_F RDB14484_A6L7b.seq
>06185_14484_A6L7_LEU1pro_F_A02_01_ABI08.ab1
    1 AATAAAGGGT GCCAACAGTG TTGAGTTTCC CGAAACCAGG CATTTTTGCG GTAGGGTCTG
   61 AAACTTTCAC GAAACCATTT CACAACAAAA CTTCTTCAAC AACATGTGTG CAAAGAAAAT
  121 CGTCGTCTTA CCAGGAGACC ATATTGGCCC TGAAATTGTT GCTTCTGCCT TGGAGGTTTT
  181 GAAAGTCGTT GAGAAGAAGC GACCTGAGTT AAAACTCGAG TTTGAAGAAC ACAAGATTGG
  241 AGGTGCCTCT ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT
  301 GGAAGCTGAC GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG
  361 TCGTCCTGAG CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG
  421 ACCTTGCAAC TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT
  481 TGAAGGTGTC GATTTTTGTG TTGTACGAGA ACTTACTGGA GGTTGTTACT TTGGTGAGCG
  541 CACTGAGGAC AACGGATCGG GTTATGCTAT GGACACTTGG CCTTACAGTT TGGAAGAAGT
  601 TTCTCGTATT GCTCGTTTGG CGGCCGCGGG CCCAACACCA ATGTTTATAA CCAAGTTTTA
  661 TCTTGTTTGT CTACATGGTA TTTTACATTC ATCTACATAC ATCTTTCATT GGCTTTGTAC
  721 ATAGTTATCA TTACAAGTCT AAAAAAAATT CACTCTTTTC TTATTCAATG TCAATCCAAG
  781 AGAAAAGATT GTGGGTAATG TTGTAGGAGC ATGTTTAATA AATTACTATA GCAAATTACT
  841 TTTTTATTCC CAAGGTGTTT ATCTATAATA GTTAATATTT TTAGTCGCTA CATAAAATTT
  901 TACCCAAAGA GTACTTGTAT ACTAAATTCT AAATGCCCTT CTGACATAAA ACGCCCTAGG
  961 AAAACAAACG CAAACAAGGC ATCGACTTTT TCAATACCAA CGAAAAATTT TACATTAGTC
 1021 CTTTTTTTTA ATGCCTGAGA AAGTCTTTGC TGGATATGCG TCGAACAGCT TCTCGATATT
1081 CTCTTGTCTT CGACAACCAG GGATTAACGA CTA
//
Primer: ars_R1 RDB14484_A6L7c.seq
>06185_14484_A6L7_ars_R1_B02_02_ABI08.ab1
    1 TGGTCCGGTT TTATACTAAA TTACTTAAGT ACCTTTAACT AAACAAAATG CCTATATATA
   61 TATTAATTTA CAATGAGTGT CAGATAAGTC ACTATGTCCG AGTGGTTAAG GAGTTAGACT
  121 CGAATTCGCA AGCTTAGCTA CAAATCCCAC TGGCTATATG TATGCATTTG TGTTAAAAAA
  181 GTTTGTATAG ATTATTTAAT CTACTCAGCA TTCTTTCTCT AAATAGGAAT TTGTTACTTA
  241 ATGGAGAAAA AAATGTTTCG ATTTACCTAG TGTATTTGTT TGTATACTCA CGTTTAATTT
  301 CAAACATCCA TTCTATCTTG TGTAATTTTT GGCATGGTGA AAAAGATAAT CAGCCTTATA
  361 ATCTTTACAA AAGTAAGAAA TTCTGTAAAT AAGCCTTAAT GCCCTTGCTT TAAATTAAAA
  421 TGGTTCTTTT TCATGATAAT GTTTGCACTT TGTGAATATA TTTTAGATAG TTCTGTGAGG
  481 TATAATTAAG ATGTTTTAGA GACTTATACA ATTTTGTCTT TATAAATTCT TAATTGATTT
  541 TACCATCCCA GTTTAACTAT GCTTCGTCGG CATCTCTGCA CATGTCGTGT TTTCTTACCG
  601 TATTGTCCTA CCAAGAACCC TCTTTTTTTG CTTGGATCGA AATTAAAGGT TTAAAAGCAA
  661 AGTTATGGGA TGCTAGAGTA TTTCAAGCTA TTCAGCTAGA GCTGAGGATG AAAAATCCAT
  721 TGCCAGGAAT TGTTGCTTGA TGAAGAAAAG CAAGCACTGG TCAGTCGCGT CGATTTGACG
  781 AAGAATTCGG AAATCTAGAA TGTTAGAATA AATGGAACCT ATGGCTGGTA TCAGACCTAT
  841 GACGTTGTCC AGGATTCGAT CAGAAATTGT GAACTAGTGC CTAGTAAAGC ACGTCTTATC
901 TTAGGATCGC
//
Primer: f1ori_R3 RDB14484_A6L7d.seq
>06185_14484_A6L7_f1ori_R3_C02_03_ABI08.ab1
    1 CGCTTAGGGT GCCTGAGTAT GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC
   61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG
  121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG
  181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG
  241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC
  301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA
  361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG
  421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA
  481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA
  541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG
  601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG
  661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA
  721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG
  781 AGATGACATG CTATTTTTCT GATTATTTTT TTTTCTATAG TTTTTCTATT TTTGTACGTC
  841 CCTTGGGGAG TCCGAAATGT AAAAATCGGG GGTATGGGTT AGTAGCGGGC AGCGTTTCAT
  901 TATGGGTATA TAAAAAGATA AAAAAGTAAG GGGCAAGCGA AGACAGAAAT GGGAATACAA
  961 AGGGCATCGT CTATAATTTA TAGCTAAAAA TTTGTATTTT AATTTGTATT TTTTTGTAAT
 1021 TTTATTCTCA ATTGTTTACT AAATGGAATG CGAATTAGAG AAACTTCGAC GAACTGTCCG
1081 AGATTAGCCT TCA
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file Sequence RDB06185z.seq from Depositor
Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)
original - reference Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original - reference Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
user report - reference Dujeancourt, L., Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria. Mitochondrion 13 (6): 871-880 (2013). PMID 23892058.
user report - reference Kuhl, I., A genome wide study in fission yeast reveals nine PPR proteins that regulate mitochondrial gene expression. Nucleic Acids Res., 39 (18): 8029-8041 (2011). PMID 21727087.

2017.02.06

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