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pDUAL2-HFF1c

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter

Catalog number RDB06154
Resource name pDUAL2-HFF1c
Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter.
Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway compatible.
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB06154]
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r, Cm^r
Gene/insert name Vector
Reference of nucleotide sequence AB364083.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
(Japanese text [open/close])
Remarks ((Remarks)) Use Survival2 (Invitrogen) or equivalent to amplify this clone.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06154 pDUAL2-HFF1c DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13298_A5Df.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB13298_A5Dfa.seq
>06154_13298_A5Df_nmt1Fw_B07_04.ab1
    1 GAATTTTAAT TAAATCAGGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
   61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
  121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
  181 ATATAAAGGA AGAGGAATCC TGGCATATCA TCAATTGAAT AAGTTGAATT AATTATTTCA
  241 ATCTCATTCT CACTTTCTGA CTTATAGTCG CTTTGTTAAA GCTAGCGTCG ACAGGCCTGG
  301 ATCCTATGCA TCACCACCAT CATCATATGG ATTATAAGGA TGATGACGAT AAACCCATGG
  361 ACTACAAGGA CGACGATGAC AAAGATATCA CAAGTTTGTA CAAAAAAGCT GAACGAGAAA
  421 CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT
  481 AATACTGTAA AACACAACAT ATCCAGTCAC TATGGCGGCC GCATTAGGCA CCCCAGGCTT
  541 TACACTTTAT GCTTCCGGCT CGTATAATGT GTGGATTTTG AGTTAGGATC CGTCGAGATT
  601 TTCAGGAGCT AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT
  661 ATCCCAATGG CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA
  721 TAACCAGACC GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA AAAATAAGCA
  781 CAAGTTTTAT CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAATT
  841 CCGTATGGCA ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC
  901 CGTTTTCCAT GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT
  961 CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA ACCCTGGTCT
 1021 ATTTCCTAAA GGGTTTATTG AGAATATGTT TTTCGTCTCA GCCAAATCCC TGCGTGAGTT
 1081 TCACAGTTTT GAATTTAACG TGCAATATGG ACACTCGTCG GCCCCCGTAT TTCACATGAA
1141 CTACTATTAA TACGGCA
//
Primer: ADH_rev RDB13298_A5Dfb.seq
>06154_13298_A5Df_ADH_rev_C07_07.ab1
    1 TAGGATCTCA GATAGATTTT CGTTTTAAAC CTAAGAGTCA CTTTAAAATT TGTATACACT
   61 TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT CATAAGAAAT TCGCTTATTT
  121 AGAAGTGGCG CGCCGGATCC TCTAGAGTCG AGATCTCATG ATATCACCAC TTTGTACAAG
  181 AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT
  241 AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG GTCGACCTGC
  301 AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC CAGAACATCA GGTTAATGGC
  361 GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT TCTTCCCCGA TAACGGAGAC
  421 CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT TCATCCCCGA TATGCACCAC
  481 CGGGTAAAGT TCACGGGAGA CTTTATCTGA CAGCAGACGT GCACTGGCCA GGGGGATCAC
  541 CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA TCCACAAACA GACGATAACG
  601 GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA CCAGCCCCTG TTCTCGTCAG
  661 CAAAAGAGCC GTTCATTTCA ATAAACCGGG CGACCTCAGC CATCCCTTCC TGATTTTCCG
  721 CTTTCCAGCG TTCGGCACGC AGACGACGGG CTTCATTCTG CATGGTTGTG CTTACCAGAC
  781 CGGAGATATT GACATCATAT ATGCCTTGAG CAACTGATAG CTGTCGCTGT CAACTGTCAC
  841 TGTAATACGC TGCTTCATAG CATACCTCTT TTTGACATAC TTCGGGTATA CATATCAGTA
  901 TATATTCTTA TACCGCAAAA ATCAGCGCGC AAATACGCAT ACTGTTATCT GGCTTTTAGT
  961 AAGCCGGGAT CCACGCGTTT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTGTAATTCA
 1021 TTTAAGCAAT TCTGGCGACA TGCAGCATCA CAAACGCAAT GATGGACTGG ATCGCAGCGG
1081 CATCAGCACT GGTCGCTTGC GTATATATTT TGCCCATGGG GTGAAC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Link to old catalog RDB06154 (link)
Electronic file Sequence RDB06154z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Reference

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
user report Toteva, T., Establishment of expression-state boundaries by Rif1 and Taz1 in fission yeast. Proc Natl Acad Sci U S A. 2017 Jan 17. pii: 201614837. doi: 10.1073/pnas.1614837114. [Epub ahead of print] PMID 28096402.

2017.06.21

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