Resource data sheet
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pCMV_S-FLAG

Expressing FLAG-tagged fusion protein, CMV promoter

Catalog number RDB05956 (link to web catalog)
Resource name pCMV_S-FLAG
Clone info. Expression vector. Expressing a fusion protein tagged with single FLAG epitope nuder the control of CMV promoter.

[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB05956]
Vector backbone pRc/CMV
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Classification Plasmid
Type_of_insert fragment Vector ()
Deposotor's page DNA Bank, | Murata, Takehide |
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication.
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Fee (non-profit org.)

We examined.

Data are summerized on test sheet below.

Test sheet RDB12327_A6Csp1.pdf

Nucleotide sequence of a portion of this resource.

Primer: CMV-Forward RDB12327_A6Csa.seq
>05956_12327_A6Cs_CMV-Forward_E01_13.ab1
    1 CTGTACACTC CGCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA
  181 GGCGGCCGCT CGAGCATGCA TCTAGAGGGC CCTATTCTAT AGTGTCACCT AAATGCTAGA
  241 GCTCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC
  301 CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG
  361 GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG
  421 GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT
  481 ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCGAGGG GGGATCCCCA CGCGCCCTGT
  541 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC
  601 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC
  661 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG
  721 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA
  781 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC
  841 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG
  901 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT
  961 AACAAAATAT TAACGCTTAC AATTTAATAT TTGCTTATAC AATCTTCCTG TTTTTGGGGC
 1021 TTTTCTGATT ATCAACCCGG GGTGGGTACG AGCTCGATTC TGTGGATGTT GTGTCAGTAA
 1081 GGTGTGAAGT CCCCAAGGCT CCCAGCAGCA GAGTATGCAA GGCATGCCAT CTCAATTTAG
1141 TCAGGCACCA G
//
Primer: SV40pro_F_V2 RDB12327_A6Csb.seq
>05956_12327_A6Cs_SV40pro_F_V2_F01_16.ab1
    1 GGTCGACGGT TTTTTTGGAG GCCTAGGCTT TTGCAAAAGC TCCCGGGAGC TTGGATATCC
   61 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA
  121 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA
  181 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT
  241 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG
  301 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA
  361 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC
  421 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT
  481 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT
  541 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG
  601 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG
  661 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA AATGGCCGCT TTTCTGGATT
  721 CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG
  781 TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT
  841 CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC
  901 GGGTGCTCTG GGGGTTCGAA ATGACCGACC AAAGCGACGC CCAACCTGCC ATCACGAGAA
  961 TTTCGATTCC ACCGCCGGCC TTTCTATGGA AAAGGTTGGG CTTTCGGGAT TCGTTTTTCC
 1021 GGGGACGCCG GGCTGGAATG ATCCTTCCAG CGCGGGGATC TTCATTGCTT GGGAGTTTCT
 1081 TCGGCCCACC TCAACCTTGG TTATTGCAAG CTAATAATGT TACAAAAAAT AAAGCCATAA
1141 GCATCACAAA TTCACAATTA AGCATTTTTT TTTTCTACAC TGTGCAAATT CCTTAGA
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file Sequence RDB05956z.seq from Depositor
Electronic file Map RDB05956a.gif from Depositor
Electronic file PDF RDB05956.pdf from Depositor
Featured content EXR0039e (English text)
Featured content EXR0039j (Japanese text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)
Featured content pCMV_tag and pRSV_tag vector (Japanese text)
Featured content pCMV_tag and pRSV_tag vector (English text)
user report - reference Hayashi, S., Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma. Exp Cell Res. 2013 May 1;319(8):1220-8. PMID 23500413.
user report - reference Shimizu, H., NF-kappaB plays an important role in indoxyl sulfate-induced cellular senescence, fibrotic gene expression, and inhibition of proliferation in proximal tubular cells. Am. J. Physiol. Cell Physiol. 301 (5): C1201-12 (2011). PMID 21832251.

2017.01.20

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