Resource data sheet
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pCMV-VSV-G-RSV-Rev

Expression vector of emvelope VSV G protein and Rev for lentivirus vector packaging.

Catalog number RDB04393
Resource name pCMV-VSV-G-RSV-Rev
Clone info. Expression vector of emvelope VSV G protein and Rev for lentivirus vector packaging.
Commonly requested with pCAG-HIVgp (RDB04394).
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB04393]
Selectable markers Amp^r
Classification Plasmid
Reference Jump to table (bottom).

Distribution information

CLICK here for plasmid map. [link]
Forms for plasmid order. [link]
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
(Japanese text [open/close])

We examined.

Data are summerized on test sheet below.

Test sheet RDB04393_A5Aqp1.pdf

Nucleotide sequence of a portion of this resource.

Primer: CMV-Foward RDB04393_A5Aqa.seq
>04393_04393_A5Aq_CMV-Forward_A04_01.ab1
    1 CTGTCACTCC GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTACA TGTGGTACCG
  181 AGCTCGGATC CTGAGAACTT CAGGGTGAGT CTATGGGACC CTTGATGTTT TCTTTCCCCT
  241 TCTTTTCTAT GGTTAAGTTC ATGTCATAGG AAGGGGAGAA GTAACAGGGT ACACATATTG
  301 ACCAAATCAG GGTAATTTTG CATTTGTAAT TTTAAAAAAT GCTTTCTTCT TTTAATATAC
  361 TTTTTTGTTT ATCTTATTTC TAATACTTTC CCTAATCTCT TTCTTTCAGG GCAATAATGA
  421 TACAATGTAT CATGCCTCTT TGCACCATTC TAAAGAATAA CAGTGATAAT TTCTGGGTTA
  481 AGGCAATAGC AATATTTCTG CATATAAATA TTTCTGCATA TAAATTGTAA CTGATGTAAG
  541 AGGTTTCATA TTGCTAATAG CAGCTACAAT CCAGCTACCA TTCTGCTTTT ATTTTATGGT
  601 TGGGATAAGG CTGGATTATT CTGAGTCCAA GCTAGGCCCT TTTGCTAATC ATGTTCATAC
  661 CTCTTATCTT CCTCCCACAG CTCCTGGGCA ACGTGCTGGT CTGTGTGCTG GCCCATCACT
  721 TTGGCAAAGA ATTCTGACAC TATGAAGTGC CTTTTGTACT TAGCCTTTTT ATTCATTGGG
  781 GTGAATTGCA AGTTCACCAT AGTTTTTCCA CACAACCAAA AAGGAAACTG GAAAAATGTT
  841 CCTTCTAATT ACCATTATTG CCCGTCAAGC TCAGATTTAA ATTGGCATAA TGACTTAATA
  901 GGCACAGCCT TACAAGTCAA AATGCCCAAG AGTCACAAAG GCTATTCAAG CAGACGGTTG
  961 GATGTGTCAT GCTTCCAAAT GGGTCACTAC TTTGTGATTT CCCGCTGGTA TGGACCGAAG
 1021 TATATAACAC ATTCCCATCC CGATCCTTTC ACTCCATCTG TAGAACAATG CAAGGAAAGG
 1081 CATTGGACAA CGAAAACAGG GAACTTGGCT GAATCCAGGC CTCCCTCCTC AAGGTTGTGG
1141 CATATGCCAC CTGTGACGGA ATGCGAAGTC ATGCAATTGT TCCAGGGGTT GAAGCT
//
Primer: BGH_rev RDB04393_A5Aqb.seq
>04393_04393_A5Aq_BGHrev_B04_04.ab1
    1 CTTTCGGGTC TCTAGACTCG AGCGGCCGCC ACTGTGCTGG ATATCTGCAG AATTCCGTTT
   61 TTTTTTTTTT TTTTCATAAA AATTAAAAAC TCAAATATAA TTGAGGCCTC TTTGAGCATG
  121 GTATCACAAG TTGATTTGGT CCAAACATGA AGAATCTGTT GTGCAGGATT TGAGTTACTT
  181 TCCAAGTCGG TTCATCTCTA TGTCTGTATA AATCTGTCTT TTCTTGGTGT GCTTTAATTT
  241 AATGCAAAGA TGGATACCAA CTCGGAGAAC CAAGAATAGT CCAATGATTA ACCCTATGAT
  301 AAAGAAAAAA GAGGCAATAG AGCTTTTCCA ACTACTGAAC CAACCTTCTA CAAGCTCGAT
  361 TGGATTTTTG GATAGCCCAG TATCACCAAA AAATAAACTC TCATCATCAG GAAGTTGCGA
  421 AGCAGCGTCT TGAATGTGAG GATGTTCGAA CACCTGAGCC TTTGAGCTAA GATGAAGATC
  481 GGAGTCCAAC ATACCATGTC CAATCATGTA TAAAGGAAAC TTATATCCTG AACTGGTCCT
  541 CAGAACTCCA TTGGGTCCAA TTTCCACGTC TTCATATGGT GCCCAGTCAT CCCACAGTTC
  601 CCTTTCTGTG GTAGTTCCAC TGATCATTCC GACCATTCTT GAGAGGATTG GAGCAGCAAT
  661 ATCGACTCTG ATGTATCTGG TCTCAAAGTA TTTTAGGGTA CCATTGATTA TGGTGAAAGC
  721 AGGACCGGTT CCTGGGTTTT TAGGAGCAAG ATAGCTGAGA TCCACTGGAG AGATTGGAAG
  781 ACCCGCTCTG ATTTTGCTCC AGGTTTCTTG GCAGAGGGAA TAATCCAAGA TCCTCTCAAC
  841 GTCCTGAATT AGACTTACAT CCACTGAGGT CTGAGATGGA GCAGAGATAC TTGACCCTTC
  901 TGGGCATTCA GGGAATCTGG CTGCAGCAAA GAGATCCTTA TCAGCCATCT CGAACCAGAC
  961 ACCTGATGGG AGTCTGACTC CCCAATGCTT TGCAGTATTG CATTTTGCAG GCCTTTGCCT
 1021 CCAGGTTTCA TAAGGCAAAG TAGTTACTTC TGAACCTGTG CCCTCCGTTT CCAGGGATGA
 1081 TAGCTTCTTC GTCCTCTGAG AGAGTTGAAT GGTCATGGCA ATGGAGGTTA GAATTCCACA
1141 TTAGCCCTTT GTGC
//
Primer: Reverse2 RDB04393_A5Aqc.seq
>04393_04393_A5Aq_Reverse2_C04_07.ab1
    1 CAGTTAATGA TTCGGAATTC GATGTACGGG CCAGATATAC GCGTATCTGA GGGGACTAGG
   61 GTGTGTTTAG GCGAAAAGCG GGGCTTCGGT TGTACGCGGT TAGGAGTCCC CTCAGGATAT
  121 AGTAGTTTCG CTTTTGCATA GGGAGGGGGA AATGTAGTCT TATGCAATAC TCTTGTAGTC
  181 TTGCAACATG GTAACGATGA GTTAGCAACA TGCCTTACAA GGAGAGAAAA AGCACCGTGC
  241 ATGCCGATTG GTGGAAGTAA GGTGGTACGA TCGTGCCTTA TTAGGAAGGC AACAGACGGG
  301 TCTGACATGG ATTGGACGAA CCACTGAATT CCGCATTGCA GAGATATTGT ATTTAAGTGC
  361 CTAGCTCGAT ACAATAAACG CCATTTGACC ATTCACCACA TTGGTGTGCA CCTCCAAGCT
  421 CGAGCTCGTT TAGTGAACCG TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC
  481 CATAGAAGAC ACCGGGACCG ATCCAGCCTC CCCTCGAAGC TAGTCGATTA GGCATCTCCT
  541 ATGGCAGGAA GAAGCGGAGA CAGCGACGAA GACCTCCTCA AGGCAGTCAG ACTCATCAAG
  601 TTTCTCTATC AAAGCAACCC ACCTCCCAAT CCCGAGGGGA CCCGACAGGC CCGAAGGAAT
  661 AGAAGAAGAA GGTGGAGAGA GAGACAGAGA CAGATCCATT CGATTAGTGA ACGGATCCTT
  721 AGCACTTATC TGGGACGATC TGCGGAGCCT GTGCCTCTTC AGCTACCACC GCTTGAGAGA
  781 CTTACTCTTG ATTGTAACGA GGATTGTGGA ACTTCTGGGA CGCAGGGGGT GGGAAGCCCT
  841 CAAATATTGG TGGAATCTCC TACAATATTG GAGTCAGGAG CTAAAGAATA GTGCTGTTAG
  901 CTTGCTCAAT GCCACAGCTA TAGCAGTAGC TGAGGGGACA GATAGGGGTT ATAGAAGTAG
  961 TACAAGAAGC TTGGGCACTG GCCGTCGTTT TACAACGTCG TGATCTGAGC CTGGGAGATC
 1021 TCTGGCTAAC TAGGGAAACC CACTGCTTAA GCCCTCAATA AAGCTTGCCC TTGAGTGCTT
 1081 CAAGTAGTGT GTGCCCGTCC TGTTGTGGTG ACTCTGGTTA CCTAGAAGAT CAAGGAAAAC
1141 CCCTTGGGCG GTTTTACCCA AACCATA
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

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2017.02.06

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