Resource data sheet
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CSII-CMV-RfA-IRES2-Venus

Shuttle vector to generate recombinant Lentiviral vector expressing venus GFP

Catalog number RDB04388
Resource name CSII-CMV-RfA-IRES2-Venus
Clone info. Shuttle vector to generate recombinant Lentiviral vector expressing venus GFP
Use DB3.1 (Invitrogen) or equivalent to amplify this clone.
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB04388]
Selectable markers Amp^r
Classification Plasmid
Type_of_insert fragment Aequorea victoria cDNA ()
Reference Jump to table (bottom).

Distribution information

CLICK here for plasmid map. [link]
Forms for plasmid order. [link]
Remarks ((Remarks)) ((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone.
(In Japanese [open/close])

We verified.

Data are summerized on test sheet below.

Test sheet RDB08941_A3Dmp1.pdf

Nucleotide sequence of a portion of this resource.

Primer: CSII-CMV5' RDB08941_A3Dma.seq
>04388_08941_A3Dm_CSII-CMV5'_A11_02.ab1
    1 ATGCACATCC AAGTTTGTAC AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA
   61 TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT
  121 CCAGTCACTA TGGCGGCCGC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG
  181 TATAATGTGT GGATTTTGAG TTAGGATCCG TCGAGATTTT CAGGAGCTAA GGAAGCTAAA
  241 ATGGAGAAAA AAATCACTGG ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA
  301 CATTTTGAGG CATTTCAGTC AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT
  361 ATTACGGCCT TTTTAAAGAC CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT
  421 CACATTCTTG CCCGCCTGAT GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT
  481 GAGCTGGTGA TATGGGATAG TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA
  541 ACGTTTTCAT CGCTCTGGAG TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT
  601 TCGCAAGATG TGGCGTGTTA CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG
  661 AATATGTTTT TCGTCTCAGC CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG
  721 GCCAATATGG ACAACTTCTT CGCCCCCGTT TTCACCATGG GCAAATATTA TACGCAAGGC
  781 GACAAGGTGC TGATGCCGCT GGCGATTCAG GTTCATCATG CCGTTTGTGA TGGCTTCCAT
  841 GTCGGCAGAA TGCTTAATGA ATTACAACAG TACTGCGATG AGTGGCAGGG CGGGGCGTAA
  901 ACGCGTGGAT CCGGCTTACT AAAAGCCAGA TAACAGTATG CGTATTTGCG CGCTGATTTT
  961 TGCGGTATAA GAATATATAC TGATATGTAT ACCCGAAGTA TGTCAAAAAA GAGGTATGCT
 1021 ATGAAAGCAG CGTATTACAG TGACAGTTTG ACAGCGACAG CTATCAGTTT GCCTCATGCA
 1081 TATATGATGT CAATATCCTC TGGTCCTGTA GCACATCAAT GCAGATGAAG CCCCGATCGT
1141 TCCTGGCCGG TTGCCCGGA
//
Primer: IRES-R RDB08941_A3Dmb.seq
>04388_08941_A3Dm_IRES-R_C11_08.ab1
    1 GCTGGCGGCT TCGGCAGTAA CGTTAGGGGG GGGGGAGGGA GAGGGGCGGA TCCGCGGCCG
   61 CTGCAGAATT CTCGAGACCG GTAGCATCAC CACTTTGTAC AAGAAAGCTG AACGAGAAAC
  121 GTAAAATGAT ATAAATATCA ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA
  181 ATACTGTAAA ACACAACATA TCCAGTCACT ATGGTCGACC TGCAGACTGG CTGTGTATAA
  241 GGGAGCCTGA CATTTATATT CCCCAGAACA TCAGGTTAAT GGCGTTTTTG ATGTCATTTT
  301 CGCGGTGGCT GAGATCAGCC ACTTCTTCCC CGATAACGGA GACCGGCACA CTGGCCATAT
  361 CGGTGGTCAT CATGCGCCAG CTTTCATCCC CGATATGCAC CACCGGGTAA AGTTCACGGG
  421 AGACTTTATC TGACAGCAGA CGTGCACTGG CCAGGGGGAT CACCATCCGT CGCCCGGGCG
  481 TGTCAATAAT ATCACTCTGT ACATCCACAA ACAGACGATA ACGGCTCTCT CTTTTATAGG
  541 TGTAAACCTT AAACTGCATT TCACCAGCCC CTGTTCTCGT CAGCAAAAGA GCCGTTCATT
  601 TCAATAAACC GGGCGACCTC AGCCATCCCT TCCTGATTTT CCGCTTTCCA GCGTTCGGCA
  661 CGCAGACGAC GGGCTTCATT CTGCATGGTT GTGCTTACCA GACCGGAGAT ATTGACATCA
  721 TATATGCCTT GAGCAACTGA TAGCTGTCGC TGTCAACTGT CACTGTAATA CGCTGCTTCA
  781 TAGCATACCT CTTTTTGACA TACTTCGGGT ATACATATCA GTATATATTC TTATACCGCA
  841 AAAATCAGCG CGCAAATACG CATACTGTTA TCTGGCTTTT AGTAAGCCGG ATCCACGCGT
  901 TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC
  961 ATGGAAAGCC ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTTG
 1021 TCGCCCTTGC GTATAATATT TGCCCATGGT GAAACGGGGG CGAAGAAGTT GTCCATATGG
 1081 CACGTTTAAA TCAAAACTGG TGGAAACTCA CCAGGAATGG CTGAGACGGA AAAAACATAA
1141 TTTCTCAAAA TATAACCTCC TTTTTAAGCG A
//
Primer: IRES-F RDB08941_A3Dmc.seq
>04388_08941_A3Dm_IRES-F_B11_05.ab1
    1 CAGTCGTTAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
   61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG
  121 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC
  181 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG
  241 CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC
  301 TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
  361 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
  421 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
  481 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC
  541 AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC
  601 AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
  661 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC
  721 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
  781 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAGAATTCAA GGCCTCTCGA
  841 GCCTCTAGAG TTAACATCGA GGGATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA
  901 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG
  961 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT
 1021 TTGTATAAAT CCTGGTTTGC TGTCTCTTTA TGAGGAGTTG TGGGCCCGTT GTCAGCAACG
 1081 TGGGCGTGAT GTGCACTGTG TTTGCTGACG CAACCCCCCA CTGATGGGGG CCATTTGACC
1141 ATCACTGTCA GGCTTCATTC CGGGGCAAAC ATAT
//
Primer: HIVgp_R1 RDB08941_A3Dmd.seq
>04388_08941_A3Dm_HIVgp_R1_D11_11.ab1
    1 CAGCTCTATT CCACTGCTCT TTTTTCTCTC TGCACCACTC TTCTCTTTGC CTTGGTGGGT
   61 GCTACTCCTA ATGGTTCAAT TTTTACTACT TTATATTTAT ATAATTCACT TCTCCAATTG
  121 TCCCTCATAT CTCCTCCTCC AGGTCTGAAG ATCAGCGGCC GGCCGCTTGC TGTGCGGTGG
  181 TCTTACTTTT GTTTTGCTCT TCCTCTATCT TGTCTAAAGC TTCCTTGGTG TCTTTTATCT
  241 CTATCCTTTG ATGCACACAA TAGAGGGTTG CTACTGTATT ATATAATGAT CTAAGTTCTT
  301 CTGATCCTGT CTGAAGGGAT GGTTGTAGCT GTCCCAGTAT TTGTCTACAG CCTTCTGATG
  361 TTTCTAACAG GCCAGGATTA ACTGCGAATC GTTCTAGCTC CCTGCTTGCC CATACTATAT
  421 GTTTTAATTT ATATTTTTTC TTTCCCCCTG GCCTTAACCG AATTTTTTCC CATCGCGATC
  481 TAATTCTCCC CCGCTTAATA CTGACGCTCT CGCACCCATC TCTCTCCTTC TAGCCTCCGC
  541 TAGTCAAAAT TTTTGGCGTA CTCACCAGTC GCCGCCCCTC GCCTCTTGCC GTGCGCGCTT
  601 CAGCAAGCCG AGTCCTGCGT CGAGAGAGCT CCTCTGGTTT CCCTTTCGCT TTCAAGTCCC
  661 TGTTCGGGCG CCACTGCTAG AGATTTTCCA CACTGACTAA AAGGGTCTGA GGGATCTCTA
  721 GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA
  781 TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC
  841 TAACCAGAGA GACCCAGTAC AGGCAAAACG CGCTGCTTAT ATAGACCTCC CACCGTACAC
  901 GCCTACCGCC CATTTGCGTC AATGGGGGCG GAGTTGTTAC GACATTTTGG AAAGTCCCGT
  961 TGATTTTGGT GCCAAAACAA ACTCCCATTG ACGTCAATGG GGGTGGAGAC TTGGAAATCC
 1021 CCGTGAGTCA AACCGCTATC CACGCCCATT GATGTACTGC CCAAAACCGC ATCACCATGG
 1081 TAATAGCGAT GACTAAATAC GTAGATGTAC TGGCTAGGTT AGAAAGGTCC CATATGTTCA
1141 TGTACTGGGG CATAATCCCA AGGGCCGGGG CCCAATA
//
Primer: BGH_rev RDB08941_A3Dme.seq
>04388_08941_A3Dm_BGHrev_E11_14.ab1
    1 GGTACGGGCT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC
   61 CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG
  121 GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC
  181 AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT GTGATGAAAT GCTAGGCGGC
  241 TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC CATCCCATGC AGGCTCACAG
  301 GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC TACCTTCTCT TGCTCAACTG
  361 GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT GATCCCTGGC CCTGGTGTGT
  421 AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT CCACAGATCA AGGATATCTT
  481 GTCTTCGTTG GGAGTGAATT AGCCCTTCCA GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA
  541 GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC
  601 CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC AGCATTGGTA GCTGCTGTAT
  661 TGCTACTTGT GATTGCTCCA TGTTTTTCTA GGTCTCGACG GTATCGATGC GGGGAGGCGG
  721 CCCAAAGGGA GATCCGACTC GTCTGAGGGC GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG
  781 CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG CTGGATTGAG GGCCGAAGGG ACGTAGCAGA
  841 AGGACGTCCC GCGCAGAATC CAGGTGGCAA CACAGGCGAG CAGCCATGGA AAGGACGTCA
  901 GCTTCCCCGA CAACACCACG GAATTGTCAG TGCCCAACAG CCGAGCCCCT GTCCAGCAGC
  961 GGGCAAAGGC AGGCGGCGAT GAGTTCCGCC GTGGCAATAG GGAGGGGGAA AGCGAAAGTC
 1021 CCGGGAAAGG AGCTGACAGG TGGTGGCAAT GCCCCAACCA GTGGGGGGTT TGCGTCAGCA
 1081 AACACAGTGC ACACCACGCC ACGTTTGCCT GACACGACAC ACTCCTCATT AAAGAGACAG
1141 CACCAAGGAT TTTTATTACC AACGGAAGGG AGAAAAAATT GA
//
Primer: SV40pro_F RDB08941_A3Dmf.seq
>04388_08941_A3Dm_SV40pro_F_F11_17.ab1
    1 GCCTGTCCTG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT
  121 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCATGGCCA
  181 AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG GTCGAGTTCT
  241 GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC GGTGTGGTCC
  301 GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG GACAACACCC
  361 TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG GAGGTCGTGT
  421 CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG CAGCCGTGGG
  481 GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG GCCGAGGAGC
  541 AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
  601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG
  661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
  721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
  781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT
  841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
  901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT
  961 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCCTGTCGTG CCAGCTGCAT
 1021 TAATGAATCG GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGGCGC TCTTCGCTTT
 1081 CTCGCCTCAC TGACTCGCTG CCGCTCCGCT CGATCCGGCT GCCGCGAAGC GTAATCCAGC
1141 TCACTCAAGC GTATACGGTT AATTCCACCA G
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

user report - reference Nakaguro, M., Nucleolar protein PES1 is a marker of neuroblastoma outcome and is associated with neuroblastoma differentiation. Cancer Sci. 106 (3): 237-243 (2015). PMID 25557119.
user report - reference Neri, S., Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma. Int. J. Cancer. 137 (4): 784-796 (2015). PMID 25648219.
user report - reference Neri, S., Cancer cell invasion driven by extracellular matrix remodeling is dependent on the properties of cancer-associated fibroblasts. J Cancer Res Clin Oncol. 2015 Sep 15. [Epub ahead of print] PMID 26374424.
user report - reference Murakami-Tonami, Y., Inactivation of SMC2 shows a synergistic lethal response in MYCN-amplified neuroblastoma cells. Cell Cycle 13 (7): 1115-1131 (2014). PMID 24553121.
user report - reference Koyama-Nasu, R., The critical role of cyclin D2 in cell cycle progression and tumorigenicity of glioblastoma stem cells. Oncogene, 32 (33): 3840-3845 (2013). PMID 22964630.
user report - reference Suzuki, T.,, Reconstruction of monocyte transcriptional regulatory network accompanies monocytic functions in human fibroblasts. PLoS One, 7 (3): e33474 (2012). PMID 22428058.
user report - reference Makinoshima, H., PTPRZ1 regulates calmodulin phosphorylation and tumor progression in small-cell lung carcinoma. BMC Cancer, 12: 537 (2012). PMID 23170925.
user report - reference Huang, P., The neuronal differentiation factor NeuroD1 downregulates the neuronal repellent factor Slit2 expression and promotes cell motility and tumor formation of neuroblastoma. Cancer Res., 71 (8), 2938-2948 (2011). PMID 21349947.

2016.07.26

GNP_filter3_RDBDEP_html_160610.pl