Resource data sheet
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CSII-CMV-MCS-IRES2-Bsd

Shuttle vector to generate recombinant Lentiviral vector expressing blasticidin.

Catalog number RDB04385
Resource name CSII-CMV-MCS-IRES2-Bsd
Clone info. Shuttle vector to generate recombinant Lentiviral vector expressing blasticidin.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB04385]
Vector backbone Plasmid
Selectable markers Amp^r

Distribution information

CLICK here for plasmid map. [link]
Forms for plasmid order [in Japanese] [in English]
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07082_A5C9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: IRES-R RDB07082_A5C9a_p1.seq
>04385_07082_4_A5C9_IRES-R_D11_11.ab1
    1 GCATGCGGCT TCGGCAGTAC GTTAGGGGGG GGGGAGGGAG AGGGGCGGAT CCGCGGCCGC
   61 TGCAGAATTC TCGAGACCGG TAGCGCTAGC GGATCTGACG GTTCACTAAA CCAGCTCTGC
  121 TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT
  181 TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA
  241 TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT
  301 GCCAAAACCG CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG
  361 GAAAGTCCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA
  421 CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT
  481 ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT ACTATGGGAA
  541 CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT
  601 TTACCGTAAG TTATGTAACG CGGAACTCCA TATATGGGCT ATGAACTAAT GACCCCGTAA
  661 TTGATTACTA TTAATCGAAT TAATTCAAAG TGAATTTCTG CTGTCCCTGT AATAAACCCG
  721 AAAATTTTGA ATTTTTGTAA TTTGTTTTTG TAATTCTTTA GTTTGTATGT CTGTTGCTAT
  781 TATGTCTACT ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCCTTTT CTTTTAAAAT
  841 TGTGGATGAA TACTGCCATT TGTAGATCCG TTCACTAATC GAATGGATCT GTCTCTGTCT
  901 CTCTCTCCAC CTTCTTCTTC TATTCCTTCG GGCCTGTCGG GTCCCCTCGG GGTTGGGAGG
  961 TGGTCTGAAC GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC
 1021 AAAAACTATT CTTAAACCTA CCAAGCCTCC TACTATCAAT TAATGATTAT TTATATACCA
1081 CAGTCCAATG TAATGCTAAT CAGTCACAAA CTTGGCCCAT TTAATTCTTA ATTTCCA
//
Primer: IRES-F RDB07082_A5C9b_p1.seq
>04385_07082_4_A5C9_IRES-F_A11_02.ab1
    1 CAGTCGTTAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
   61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGCCAA GCCTTTGTCT
  121 CAAGAAGAAT CCACCCTCAT TGAAAGAGCA ACGGCTACAA TCAACAGCAT CCCCATCTCT
  181 GAAGACTACA GCGTCGCCAG CGCAGCTCTC TCTAGCGACG GCCGCATCTT CACTGGTGTC
  241 AATGTATATC ATTTTACTGG GGGACCTTGT GCAGAACTCG TGGTGCTGGG CACTGCTGCT
  301 GCTGCGGCAG CTGGCAACCT GACTTGTATC GTCGCGATCG GAAATGAGAA CAGGGGCATC
  361 TTGAGCCCCT GCGGACGGTG CCGACAGGTG CTTCTCGATC TGCATCCTGG GATCAAAGCC
  421 ATAGTGAAGG ACAGTGATGG ACAGCCGACG GCAGTTGGGA TTCGTGAATT GCTGCCCTCT
  481 GGTTATGTGT GGGAGGGCTA AGTCGAGCCT CTAGAGTTAA CATCGAGGGA TCAAGCTTAT
  541 CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT
  601 TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC
  661 CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA
  721 GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC
  781 CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT
  841 CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG
  901 GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT
  961 GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC
 1021 CCTCAATCCA GCGGACTTTC CTTTCCCGCG GCCTGCTGCC GGGCTCTGCC GACTCCTTCG
 1081 CCGTCCTTTC ACTTCGATCG TCAGACGAAG TCCGATCTCC ATTTTGGGAC GACATCCCCG
1141 GCAATTCGGA ATACCGCGT
//
Primer: HIVgp_R1 RDB07082_A5C9c_p1.seq
>04385_07082_4_A5C9_HIVgp_R1_B11_05.ab1
    1 CAGCTCTATT CCACTGCTCT TTTTTCTCTC TGCACCACTC TTCTCTTTGC CTTGGTGGGT
   61 GCTACTCCTA ATGGTTCAAT TTTTACTACT TTATATTTAT ATAATTCACT TCTCCAATTG
  121 TCCCTCATAT CTCCTCCTCC AGGTCTGAAG ATCAGCGGCC GGCCGCTTGC TGTGCGGTGG
  181 TCTTACTTTT GTTTTGCTCT TCCTCTATCT TGTCTAAAGC TTCCTTGGTG TCTTTTATCT
  241 CTATCCTTTG ATGCACACAA TAGAGGGTTG CTACTGTATT ATATAATGAT CTAAGTTCTT
  301 CTGATCCTGT CTGAAGGGAT GGTTGTAGCT GTCCCAGTAT TTGTCTACAG CCTTCTGATG
  361 TTTCTAACAG GCCAGGATTA ACTGCGAATC GTTCTAGCTC CCTGCTTGCC CATACTATAT
  421 GTTTTAATTT ATATTTTTTC TTTCCCCCTG GCCTTAACCG AATTTTTTCC CATCGCGATC
  481 TAATTCTCCC CCGCTTAATA CTGACGCTCT CGCACCCATC TCTCTCCTTC TAGCCTCCGC
  541 TAGTCAAAAT TTTTGGCGTA CTCACCAGTC GCCGCCCCTC GCCTCTTGCC GTGCGCGCTT
  601 CAGCAAGCCG AGTCCTGCGT CGAGAGAGCT CCTCTGGTTT CCCTTTCGCT TTCAAGTCCC
  661 TGTTCGGGCG CCACTGCTAG AGATTTTCCA CACTGACTAA AAGGGTCTGA GGGATCTCTA
  721 GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA
  781 TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC
  841 TAACCAGAGA GACCCAGTAC AGGCAAAACG CGCTGCTTAT ATAGACCTCC CACCGTACAC
  901 GCCTACCGCC CATTTGCGTC AATGGGGCGG AGTTGTTACG ACATTTTGGA AAGTCCCGTT
  961 GATTTTGGTG CCAAAACAAA CTCCCATTGA CGTCAATGGG GTGGAGACTT GGAAATCCCC
 1021 GTGAGTCAAA CCGCTATCCA CGCCCATTGA TGTACTGCCC AAAACCGCAT CACCATGCTT
 1081 ATAGCGATGA CTAATACGTA GATGTACCTG ACTAGTTAGG AAGTCCCATA AGTTCATTGT
1141 TACCTTGGGG GCCATCA
//
Primer: BGH_rev RDB07082_A5C9d_p1.seq
>04385_07082_4_A5C9_BGHrev_C11_08.ab1
    1 GTTCGGGACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC
   61 CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG
  121 GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC
  181 AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT GTGATGAAAT GCTAGGCGGC
  241 TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC CATCCCATGC AGGCTCACAG
  301 GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC TACCTTCTCT TGCTCAACTG
  361 GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT GATCCCTGGC CCTGGTGTGT
  421 AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT CCACAGATCA AGGATATCTT
  481 GTCTTCGTTG GGAGTGAATT AGCCCTTCCA GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA
  541 GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC
  601 CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC AGCATTGGTA GCTGCTGTAT
  661 TGCTACTTGT GATTGCTCCA TGTTTTTCTA GGTCTCGACG GTATCGATGC GGGGAGGCGG
  721 CCCAAAGGGA GATCCGACTC GTCTGAGGGC GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG
  781 CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG CTGGATTGAG GGCCGAAGGG ACGTAGCAGA
  841 AGGACGTCCC GCGCAGAATC CAGGTGGCAA CACAGGCGAG CAGCCATGGA AAGGACGTCA
  901 GCTTCCCCGA CAACACCACG GAATTGTCAG TGCCCAACAG CCGAGCCCCT GTCCAGCAGC
  961 GGGCAAAGGC AGGCGGCGAT GAGTTCCGCC GTGGCCATAG GGAGGGGGAA AGCGAAAGTC
 1021 CCGGGAAGGA GCTGACAGGT GGTGGCAATG CCCCACCAGT GGGGGGTTTG CGTCAGCAAA
 1081 CACAGTGCAC ACCACCGCTC ACGTTGCGTG ACACGGATCA CACTTCGTCA TAAGAAGAAC
1141 AAGCAAACCA AGGAAATTTT ATATACCAAA AG
//
Primer: SV40pro_F RDB07082_A5C9e_p1.seq
>04385_07082_4_A5C9_SV40pro_F_E11_14.ab1
    1 CCTGGTCTTG CATTCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGTTGA CAATTAATCA
  121 TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG
  181 TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT CGAGTTCTGG
  241 ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG TGTGGTCCGG
  301 GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA CAACACCCTG
  361 GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA GGTCGTGTCC
  421 ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA GCCGTGGGGG
  481 CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC CGAGGAGCAG
  541 GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
  601 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
  661 TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
  721 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
  781 CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA
  841 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA
  901 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
  961 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA
 1021 TGAAATCGGC CAACGCGCGG GGGAGAGGCG GTTTGCGTAT TGGCGCTCTT CGCTTCCTCG
 1081 CTCACTGACT CGCTGCGCTT CGATTCGATC CGGCCTGCCG CCGAGCCGGC TATCAGTCTT
1141 CACACCTTCA AAAGGG
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Reference

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2017.06.14

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