Resource data sheet
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CSII-EF-MCS-IRES2-Venus

Shuttle vector to generate recombinant Lentiviral vector expressing venus GFP.

Catalog number RDB04384
Resource name CSII-EF-MCS-IRES2-Venus
Clone info. Shuttle vector to generate recombinant Lentiviral vector expressing venus GFP.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB04384]
Vector backbone Plasmid
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA

Distribution information

CLICK here for plasmid map. [link]
Forms for plasmid order [in Japanese] [in English]
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13022_A4L1p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: IRES-R RDB13022_A4L1a.seq
>04384_13022_A4L1_IRES-R_A01_01.ab1
    1 CCATTAACGG CCTTCGGCAG TAACGTTAGG GGGGGGGGAG GGAGAGGGGC GGATCCGCGG
   61 CCGCTGCAGA ATTCTCGAGA CCGGTAGCGT GTTCACGACA CCTGAAATGG AAGAAAAAAA
  121 CTTTGAACCA CTGTCTGAGG CTTGAGAATG AACCAAGATC CAAACTCAAA AAGGGCAAAT
  181 TCCAAGGAGA ATTACATCAA GTGCCAAGCT GGCCTAACTT CAGTCTCCAC CCACTCAGTG
  241 TGGGGAAACT CCATCGCATA AAACCCCTCC CCCCAACCTA AAGACGACGT ACTCCAAAAG
  301 CTCCAGAACT AATCGAGGTG CCTGGACGGC GCCCGGTACT CCGTGGAGTC ACATGAAGCG
  361 ACGGCTGAGG ACGGAAAGGC CCCTTTCCTT TGTGTGGGTG ACTCACCCGC CCGCTCTCCC
  421 GAGCGCCGCG TCCTCCATTT TGAGCCCCCT GGAGCAGGGC CGGGAAGCGG CCATCTTTCC
  481 GCTCACGCAA CTGGTGCCGA CCGGGCCAGC CTTGCCGCCC AGGGCGGGGC GATACACGGC
  541 GGCGCGAGGC CAGGCACCAG AGCAGGCCGG CCAGCTTGAG ACTACCCCCG TCCGATTCTC
  601 GGTGGCCGCG CTCGCAGGCC CCGCCTCGCC GAACATGTGC GCTGGGACGC ACGGGCCCCG
  661 TCGCCGCCCG CGGCCCCAAA AACCGAAATA CCAGTGTGCA GATCCTGGCC CGCATTTACA
  721 AGACTATCTT GCCAGAAAAA AAGCGTCGCA GCAGGTCATC AAAAATTTTA AATGGCTAGA
  781 GACTTATCGA AAGCAGCGAG ACAGGCGCGA AGGTGCCACC AGATTCGCAC GCGGCGGCCC
  841 CAGCGCCCAG GCCAGGCCTC AACTCAAGCA CGAGGCGAAG GGGCTCCTAA AGCGCAAGGC
  901 CCGCCCCTGG CTCCAGCTCG GGATCAAGAA TCACGTACTG GAGCCAGGTG GAAGTAATTC
  961 AAGGCACGCA AGGGCCATAA CCCGTAAAAG AGGCCAGGCC CGCGGGAACC ACACACGGCA
 1021 CTTACCTGTG TTCTGGCGGC AAACCCGTTG CGAAAAGACG TTTCACGGCG ACTACTGCAC
 1081 TTATATACCG TCTCCCCCCA ACCCTCGGAA AAGCGAGCCA GTACACGACA TCACTTTCCC
1141 AGTTAACCCC GCGCCACTTC CTCTAAGGGG CA
//
Primer: IRES-F RDB13022_A4L1b.seq
>04384_13022_A4L1_IRES-F_B01_04.ab1
    1 TCTGGCCTTT TAATGTGTTT TAGGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC
   61 GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAATATG GCCACAACCA TGGTGAGCAA
  121 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
  181 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC
  241 CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
  301 CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT
  361 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
  421 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
  481 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
  541 CAACTACAAC AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC
  601 CAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA
  661 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA
  721 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT
  781 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAG AATTCAAGGC
  841 CTCTCGAGCC TCTAGAGTTA ACATCGAGGG ATCAAGCTTA TCGATAATCA ACCTCTGGAT
  901 TACAAAATTT GTGAAAAGAT TGACTGGTAT TCTTAACTAT GTTTGCTCCT TTTTACGCTA
  961 TGTGGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTTCCCGT ATGGCTTTCA
 1021 TTTTCTCCTC CTTGTAATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGTCCGTTG
 1081 TTCAGGCAAC GTGGCGTGGT GGTGCACTGT GTTGGCCTGA CGCAACCCCC CAACTTGTTG
1141 GGGCCATGGC CCACCTAACC TGTGTCAGCC TCCCCTAT
//
Primer: BGH_rev RDB13022_A4L1c.seq
>04384_13022_A4L1_BGHrev_C01_07.ab1
    1 CGCTACGGGG TCTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
   61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
  121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
  181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC
  241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA
  301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA
  361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG
  421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT
  481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC
  541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA
  601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG
  661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG
  721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA
  781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
  841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG
  901 TCAGCTTCCC CGACAACACC ACGGAATTGT CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC
  961 AGCGGGCAAA GGCAGGCGGC GATGAGTTCC GCCGTGGCAA TAGGGAGGGG GAAAGCGAAA
 1021 GTCCCGGGAA AGGAGCTGAC AGTGGTGGCA ATGCCCCAAC CCAGTGGGGG TTGCGTCAGC
 1081 AAACACAGTG CACACACGCA CGTTGCTGAC AACGGACCAC ACTCTCATAA GAGACAGCAC
1141 AGAATTTATA CACGAGGAAG AAAAATGAAA GGCCCAATAG
//
Primer: CMV-Forward RDB13022_A4L1d.seq
>04384_13022_A4L1_CMV-Forward_D01_10.ab1
    1 CTGTACAACC TCCGGCCCCC CTTTGACCGC AAATGGGCGG TAGGCGTTGT ACGGTGGGAG
   61 GTCTATATAA GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC
  121 CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG
  181 AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG
  241 ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG
  301 AAAGGGAAAC CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA
  361 AGAGGCGAGG GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG
  421 GAGAGAGATG GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA
  481 AAAATTCGGT TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA
  541 AGCAGGGAGC TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT
  601 AGACAAATAC TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA
  661 TTATATAATA CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC
  721 AAGGAAGCTT TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA
  781 GGGCCGCTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA
  841 TATAAATATA AAGTAGTAAA AAATTGAACC ATTAGGAGTT AGCACCCACC AAGGCAAAAG
  901 AGAAGAGTGG TGCAGAGAGA AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGGT
  961 TCTTGGGAGC AGCAGGAAGC ACTATGGGCG CAGCCGTCAA TGACGCTGAC GGTACAGGCC
 1021 AGACAATTAT TGGTCTGGTA TAGTGGCAGC AGCCAGAACA ATTTGCTGGA GGGCCTAATT
1081 GGAGGGCGCA AACAGCAATC TGGTTGGCAA CC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Reference

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2017.06.20

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