Resource data sheet
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CSII-CMV-MCS

Shuttle vector to generate recombinant Lentiviral vector.

Catalog number RDB04377
Resource name CSII-CMV-MCS
Clone info. Shuttle vector to generate recombinant Lentiviral vector.

[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB04377]
Selectable markers Amp^r
Classification Plasmid
Type_of_insert fragment Vector ()
Reference Jump to table (bottom).

Distribution information

CLICK here for plasmid map. [link]
Forms for plasmid order. [link]

We verified.

Data are summerized on test sheet below.

Test sheet RDB04377_A3Fb.pdf

Nucleotide sequence of a portion of this resource.

Primer: CSII-CMV5' RDB04377_A3Fba.seq
>04377_04377_A3Fb_CSII-CMV5'_C09_09.ab1
    1 GATGCAACGC TCGGTCTCGA GATTCTGCAG CGGCCGCGGA TCCTCTAGAG TTAACATCGA
   61 GGGATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT
  121 ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT
  181 CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG
  241 TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT
  301 GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT
  361 TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC
  421 TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG
  481 TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC
  541 TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG
  601 CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC
  661 TCCCCGCATC GATACCGTCG AGACCTAGAA AAACATGGAG CAATCACAAG TAGCAATACA
  721 GCAGCTACCA ATGCTGATTG TGCCTGGCTA GAAGCACAAG AGGAGGAGGA GGTGGGTTTT
  781 CCAGTCACAC CTCATGTACC TTTAAGACCA ATGACTTACA AAGGCAGCTG TAGATCTTAG
  841 CCACTTTTTA AAAGAAAAGG GGGGACTGGA AAGGGCTAAT TCACTCCCAA CGAAGACAAG
  901 ATATCCTTGA TCTGTGGATC TACCACACAC AAAGGCTACT TCCCTGATTG GCAGAACTAC
  961 ACACCAGGGC CCAGGGATCA GATATCCACT GACCTTTGGA TGGTGCTACA GCTAGTACCA
 1021 GTTGAGCAGA GAACGTAGAG AGCATGATGA GAGACACCCC GCTGGTACAC CTGTGAGCTT
1081 GCATGGCATG GATGACCAGA AAGAAGAAGT AATTAAAGGT GGAAGGATGA
//
Primer: BGH_rev RDB04377_A3Fbb.seq
>04377_04377_A3Fb_BGH_rev_F03_18.ab1
    1 CATAACCGGG GACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG
   61 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT
  121 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT
  181 AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG CCATGTGATG AAATGCTAGG
  241 CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT CATCCATCCC ATGCAGGCTC
  301 ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT CTTCTACCTT CTCTTGCTCA
  361 ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGATCCC TGGCCCTGGT
  421 GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT AGATCCACAG ATCAAGGATA
  481 TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG
  541 CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG GTACCTGAGG TGTGACTGGA
  601 AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT
  661 GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACGGTATCG ATGCGGGGAG
  721 GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC GAAGACGCGG AAGAGGCCGC
  781 AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG TCCGCTGGAT TGAGGGCCGA AGGGACGTAG
  841 CAGAAGGACG TCCCGCGCAG AATCCAGGTG GCAACACAGG CGAGCAGCCA TGGAAAGGAC
  901 GTCAGCTTCC CCGACAACAC CACGGAATTG TCAGTGCCCA ACAGCCGAGC CCCTGTCCAG
  961 CAGCGGGCAA AGGCAGGCGG CGATGAGTTC CGCCGTGGCA ATAGGGAGGG GGAAAGCGAA
 1021 AGTCCCGGAA GGAGCTGACA GGTGGTGGCA ATGCCCCACC AGTGGGGGGT TGCGTCAGCA
 1081 AACACAGTGC ACACCACGCC ACGTTGCCTG ACACGGCCAC ACTCTCATAA GAGACAGCAC
1141 AAGGAATTTA TACACGAGGG AGAAAAATGG AAAAGC
//
Primer: HIVgp_R1 RDB04377_A3Fbc.seq
>04377_04377_A3Fb_HIVgp_R1_C07_07.ab1
    1 CAGCCACTCT ATTTCCCACC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTTG
   61 GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC
  121 CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGGCC GCTTGCTGTG
  181 CGGTGGTCTT ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT
  241 TTATCTCTAT CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA
  301 GTTCTTCTGA TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT
  361 CTGATGTTTC TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA
  421 CTATATGTTT TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC
  481 GCGATCTAAT TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC
  541 CTCCGCTAGT CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC
  601 GCGCTTCAGC AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA
  661 AGTCCCTGTT CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA
  721 TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA
  781 GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT
  841 CTGGTCTAAC CAGAGAGACC CAGTACAGGC AAAACGCGCT GCTTATATAG ACCTCCCACC
  901 GTACACGCCT ACCCGCCCAT TTGCGTCAAT GGGGGCGGAG TTGTTACGAC ATTTTGGAAA
  961 AGTCCCGTTG ATTTTGGGTG CCAAACAAAC TCCCCATTGA CGTCAATGGG TGGAGACTTT
 1021 GGAAATCCCC GTGAGTCAAC CGCTATCCAC GCCCCATTGA TGTACTGCCA AAACCGCATC
 1081 CACCCATGGT AATAGCCGAT GACCTATACC GTAGATGTAC TGCCTAGTTA GAAAGTTCCC
1141 CATTAAGTTC AATGTTAACC TTGGGGCCAT ATAAATGGCG A
//
Primer: SV40pro_F RDB04377_A3Fbd.seq
>04377_04377_A3Fb_SV40pro_F_D07_10.ab1
    1 CTCCTTGCCC GCTGGCCTAT CCAGAAGTAG TTGAGGAGGC TTTTTTGGAG GCCTAGGCTT
   61 TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAGC ACGTGTTGAC
  121 AATTAATCAT CGGCATAGTA TATCGGCATA GTATAATACG ACAAGGTGAG GAACTAAACC
  181 ATGGCCAAGT TGACCAGTGC CGTTCCGGTG CTCACCGCGC GCGACGTCGC CGGAGCGGTC
  241 GAGTTCTGGA CCGACCGGCT CGGGTTCTCC CGGGACTTCG TGGAGGACGA CTTCGCCGGT
  301 GTGGTCCGGG ACGACGTGAC CCTGTTCATC AGCGCGGTCC AGGACCAGGT GGTGCCGGAC
  361 AACACCCTGG CCTGGGTGTG GGTGCGCGGC CTGGACGAGC TGTACGCCGA GTGGTCGGAG
  421 GTCGTGTCCA CGAACTTCCG GGACGCCTCC GGGCCGGCCA TGACCGAGAT CGGCGAGCAG
  481 CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC
  541 GAGGAGCAGG ACTGACACGT GCTACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG
  601 TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC
  661 ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAAATG GTTACAAATA
  721 AAGCAAATAG CATCACAAAT TTCACAAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT
  781 GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG
  841 AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC CGCTCACAAT
  901 TCCACACAAC ATACGAGGCC GGAAGCATAA AGTGTAAAGC CTGGGGGTGC CTAATGAGTG
  961 AGCTAACTCA CATTAATTTG CGTTGCGCTC ACTGGCCCGC TTTCCAAGTC GGGGAAACCT
 1021 GTCGTGCCCA GCTGCATTAA ATGAATCCGG CCCAACGCCG CGGGGAGAGG CGGGTTTGCG
 1081 TATTTTGGCG CCTTCTTTTC CGCTTTCCTT CGCCTTCACA CTTGACTCGC TGCGCTTCGG
 1141 TTCGGTTTCG GGCCTGGCCG GCCGAAGGCC GGGTTATTAT TACAAAGCCT TTCATAACAC
1201 T
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

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user report - reference Watanabe, T., FBL2 regulates amyloid precursor protein (APP) metabolism by promoting ubiquitination-dependent APP degradation and inhibition of APP endocytosis. J. Neurosci., 32 (10): 3352-3365 (2012). PMID 22399757.
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2016.07.26

GNP_filter3_RDBDEP_html_160610.pl