Resource data sheet
Prev.

pCMV-MAZ

Human MAZ cDNA is cloned into EcoRI site of pcDNA3

Catalog number RDB03219 (link to web catalog)
Resource name pCMV-MAZ
Alternative name pCMV-MAZi
Clone info. Human MAZ cDNA is cloned into EcoRI site of pcDNA3.

[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB03219]
Vector backbone pcDNA3
Size of vector backbone 5.446 bp
Selectable markers Amp^r
Classification Plasmid
Type_of_insert fragment Human cDNA (islet)
Other clones with this gene 4150 | (indicated in NCBI Gene ID)
Deposotor's page Yokoyama, Kazunari | Song, Jun |
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions for distribution Manuscripts for the publication shall be made through the discussion between Developer/Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Biochem. Biophys. Res. Commun., 226, 801-809 (1996).) Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.
(In Japanese [open/close])
Fee (non-profit org.)

We examined.

Data are summerized on test sheet below.

Test sheet RDB12831_A4Gp.pdf

Nucleotide sequence of a portion of this resource.

Primer: CMV-Foward RDB12831_A4Gpa.seq
>03219_12831_A4Gp_CMV-Foward_A01_01.ab1
    1 ACGAAATCCC TTCCCGTCCC CAATTGAGGC AAATGGGCGG TAGGCAGTGC CCCGGTGGGA
   61 GGTCTAAATA AGCAGAGCTT CTGAAATAAA TAGAGAACCC ACTGCTTACT GGCTTATCGA
  121 AATTAATACT ACTCACTATA GGGAGACCCA AGCTTGGTAC CGAGCTCGGA TCCACTAGTA
  181 ACGGCCGCCA GTGTGCTGGA ATTCCCGGGG TCGCCGCGGC GCTCCGCGGC CCGCAAGGCG
  241 CCCTCCTTTC CTCCCTCCCG CCGGCCGGGG TGCGCGGGCG GCGGGGCGGC CCGCGGGCCA
  301 TGCGTTCGGC GCGGCCCAGC CCGGCCGGCC GGGGGCGGCG CCCCCGGCCC CCGCTGAGCC
  361 CTGGGGGCCC CGCTGCGGCC GAGGCCATGT TCCCGGTGTT TCCTTGCACG CTGCTGGCCC
  421 CCCCCTTCCC CGTGCTGGGC CTGGACTCCC GGGGGGTGGG CGGCCTCATG AACTCCTTCC
  481 CGCCACCTCA GGGTCACGCC CAGAACCTCC TGCAGGTCGG GGCTTAAGCT CCAGTCCCGC
  541 TTCTTGCCTC CCAGGGCTGC GCCCAGAGTC CATTCCAGGC CGCGCCGGCG CCCCGCCACG
  601 CCCCAGGCCC GGGGCCGAAC CCCCCAGTGA AATTGCTCCG TTTTCCCGTC CCCAAGATTC
  661 CCCGCGGTTG CTGGGCCCTG CGCCCATGCT GCCCCCGTCT TCCGGCCCCG GCCTTCCGCG
  721 CTTTTTTGGG GAAAAAGCGG CCCTAAAGAC CTTCGGGCCC TTCCCCCCCC CCCCCCCCAG
  781 GGTGGGGGCC CCGGGCCAAG GGCGGGCCCC CCCCCTCCCC CCCCCCAATT TTGCGGGGGG
  841 GGGGGGGCCC ACGCGGTTAT AACCCCAACC TTCAAAGGGT GCCGGGGGGC CCCCGGTGCG
  901 GTTTTTCTTT TGGGAAAAAA AAAAAAAAAG AGAGAGGGGG GCCCCACTTT TTCCCCCTTT
  961 TTGTGGCCCC CAGGGAGATT TTAAAAAGGG ATTAAATTTT GTGGGGAGGA GGGAGAAAAT
 1021 CTATCCTAAC AGGAAGAAAG AGGGGGGGGC GGGGGTCCCC TCCCCGGGGG GGATTAAAAA
 1081 AAAGCACAAC AGTGGGGGTG CTCTATCATG TATCTCTCTA TGGAGCGGCC CCCCCTTCAA
 1141 TAAATGATAA CAACCTGCGG ATGTGGAGTG CGAAGTAAAG TACCAGGGTG TTGGGGGTGT
1201 GTTCCGGCGC GCGTATC
//
Primer: BGH_rev RDB12831_A4Gpb.seq
>03219_12831_A4Gp_BGH_rev_B01_04.ab1
    1 GTTCCTATCC TTTAAGGGTG AACTATAGAA TAGGGCCCTC TAGATGCATG CTCGAGCGGC
   61 CGCCAGTGTG ATGGATATCT GCAGAATTCC CCTAAGAAAA CATTTCTTCC TCCTTTCCTT
  121 CTGGAGGCTC CTTGGTTGGT AGGGAAATAG GAGTTGGTGG GAAAAGAGGG GGGAGAGGAG
  181 CTTGTACCAA GGGACTCTAC TCCATTCTCC CCTCTCCCCC GCAACCAACT TGGAGCTCAC
  241 CAGGGTTGGG AGGGAAGTGG CTGAGAGCTC ACAGGCACCC CCTCCGCCCC CGAGAGGGAG
  301 CCCACAGCTG TGGGAGGGGC TGCTACTGCT GCCGCTGCTG CCGCTGCCGC CGCCATTGGA
  361 CAAACCTCAC CAGTACCTTT GTTGCAGAGC TCACAGACAT GGTGAGGACC CTGGCTGTGC
  421 ACCTTCATGT GGTCCGAAAT ATAAGCCGAG CTCAGCATCT TGCCACACAC GTGACATGGC
  481 ACTTTCTCCT CGTGTCGTAC TGTGTGCGCC CGCAGCCGAT CCTTCGTGGC GAAAGCTGCC
  541 TCACATTTCT CACATTTGAA GGGCCGTTCT GTTGAGTGCA CTTGTCTGAC GTGACTGTTG
  601 AGGTGATCCG GCCGGGAGAA GCTCTTGCCA CAGTGGGAGC AGTTGTAGGG CTTGTGCACA
  661 GCGCCGTCAT GTGAGCGCAC GTGGTAGCTC ATGCGGTCCT TGCGCTTGAA GCGCTGCTGG
  721 CACACCGGGC ACTGGTAGGG CTTCTCGTCC GAGTGCGACA GCTTGTGTCG GTTCAGGTGG
  781 TAGACGTCGC GGAAGGCCTT GCCACACATC TCGCAGGCAT GGTTCTTCCG GATGCGCTTC
  841 CCCGAGGCGG TCGTGGTCAC CACGCCACCG GCGGCCACTG CGGCAGCGCC GCCGCCGGCA
  901 CCCGCCTCTC CCCCTCCCCC GCCGGCTCCG CTCAGCTGGG GCACGCTCAG AGGCTCAGGG
  961 CACCATGGTC GGCATCTTCA TAGCACCCGA GGGGACCCGG GCCGGGCCTT GGCTCCGTGT
 1021 GATGGCTTCG TTGCCTCCGG AGATTGTAGC CCGTCTTGAA CTCTGCGCCA CAGAGCGCAG
 1081 ATGTAGCCCT GCTCTTGTCC TTCTCTCAGG CAGACGCGAT CGGCCCACGG CGAACGTCGA
1141 GTTTTGGGGG GCCTACGCA
//
Primer: SV40pro_F RDB12831_A4Gpc.seq
>03219_12831_A4Gp_SV40pro_F_C01_07.ab1
    1 CCCCCAATCC TTGACTTTTC CGAAGTACTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
   61 CAAAAAGCTC CCGGGAGCTT GTAAAACCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
  121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
  181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
  241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
  301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
  361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
  421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
  481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
  541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
  601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
  661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
  721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
  781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
  841 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
  901 TCGCCTTCTT GACGAGTTCT TTCTGAGCGG GACTCTGGGG TTTCGAAATG ACCGACCAAG
  961 CGACGCCCAA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCCTTCT ATGAAAGGTT
 1021 GGGGCTTCGG AATTCGGTTT TTCCGGGACG CCCGGCTGGA ATGATTCTTC CAGCGCGGGG
 1081 AATCTTCATG CCTGGAGTTC TTTCCACCAC CCCAAACTTG ATTTATTGCC AGCCTTATAT
1141 TGTACAATTA AAGGCAATAG GCCATTCACC AAATTCACAA AATAACAGCC CACT
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

original - reference , Biochem. Biophys. Res. Commun., 226, 801-809 (1996). PMID 8831693.
user report - reference Cogoi, S., MAZ-binding G4-decoy with locked nucleic acid and twisted intercalating nucleic acid modifications suppresses KRAS in pancreatic cancer cells and delays tumor growth in mice. Nucleic Acids Res., 41(7):4049-4064 (2013). PMID 23471001.
user report - reference Tsui K.H., Mechanisms by which Interleukin-6 Regulates Prostate Specific Antigen Gene Expression in Prostate LNCaP Carcinoma Cells. J. Androl. 32 (4): 383-393 (2011). PMID 21051589.
user report - reference Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711.
user report - reference Milena, M., Involvement of ubiquitous and tale transcription factors, as well as liganded RXR alpha, in the regulation of human SOX2 gene expression in the NT2/D1 embryonal carcinoma cell line. Arch. Biol. Sci., Belgrade, 62, 199-210 (2010).
user report - reference Cogoi S., The KRAS promoter responds to Myc-associated zinc finger and poly(ADP-ribose) polymerase 1 proteins, which recognize a critical quadruplex-forming GA-element. J. Biol. Chem., 285, 22003-22016 (2010). PMID 20457603.
user report - reference Frensing T., Characterization of a neuregulin-1 gene promoter: positive regulation of type I isoforms by NF-kappaB. Biochim. Biophys. Acta - Gene Regul. Mech., 1779, 139-144 (2008). PMID 18082154.

2016.09.23

GNP_filter3_RDBDEP_html_160913.pl