Resource data sheet
Prev.

pCMX-N/RBP-J

Expression vector of mouse RBP-J

Catalog number RDB03021 (link to web catalog)
Resource name pCMX-N/RBP-J
Alternative name pCMX-N/RBP-2
Clone info. Expression vector of mouse RBP-J under the control of CMV promoter. Dominant negative form [pCMX-N/RBP-J (R218H), RDB03022] and constitutive active form (pCMX-N/VP16-RBP-J, RDB03023) are available.

[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB03021]
Vector backbone pCMX-N
Size of vector backbone 4,536 bp
Selectable markers Amp^r
Classification Plasmid
Type_of_insert fragment Mouse cDNA ()
Other clones with this gene 19664 | (indicated in NCBI Gene ID)
Deposotor's page Honjo, Tasuku | Kuroda, Kazuki |
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions for distribution For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@phy.med.kyoto-u.ac.jp). Acknowledgement to Depositor in publication. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
(In Japanese [open/close])
Fee (non-profit org.)

We examined.

Data are summerized on test sheet below.

Test sheet RDB12301_A3Jm.pdf

Nucleotide sequence of a portion of this resource.

Primer: RDB12301_A3Jma.seq
>03021_12301_A3Jm_T7-20bp_C10_07.ab1
    1 CACAATGTCT CTAGAGGATA TCGAATTCCT GCAGCCCAAT ATGCCCTCCG GTTTTCCTCA
   61 GTCTCCACGT ACGTCCCCGA GGGCGCGTCC CAAAACCCGG ATAACCGGAG CGCTCCCCAT
  121 GGACTACTCG GAGGGCTTGT CCGCGGAGGA GCGGCCTGCG CATGCTCCAT CGGCGGGGAA
  181 GTTTGGTGAG CGGCCTCCAC CCAAACGACT CACTAGGGAA GCTATGCGAA ATTATTTAAA
  241 AGAACGAGGG GATCAAACAG TGCTCATTCT TCATGCAAAA GTTGCACAGA AGTCTTACGG
  301 AAATGAAAAA CGATTTTTTT GCCCTCCTCC TTGTGTGTAT CTTATGGGCA GTGGTTGGAA
  361 GAAAAAAAAA GAGCAAATGG AACGAGATGG ATGTTCTGAA CAAGAGTCTC AACCCTGTGC
  421 GTTTATTGGA ATAGGAAACA GTGACCAAGA AATGCAGCAG CTGAACCTCG AGGGGAAGAA
  481 CTACTGCACA GCCAAAACAT TGTACATATC TGATTCAGAC AAGAGAAAAC ATTTCATGTT
  541 GTCTGTAAAG ATGTTCTATG GCAACAGCGA TGACATTGGT GTGTTCCTCA GCAAGCGGAT
  601 AAAGGTCATC TCCAAACCCT CCAAAAAGAA GCAGTCACTG AAGAATGCTG ACTTGTGCAT
  661 TGCTTCAGGA ACGAAGGTGG CACTGTTCAA TCGCCTTCGG TCCCAGACAG TTAGTACCAG
  721 GTACCTGCAT GTAGAAGGAG GGAATTTCCA CGCCAGTTCA CAACAGTGGG GAGCATTTTA
  781 CATCCATCTC TTGGACGACG ACGAGTCGGA AGGAGAGGAG TTCACAGTTA GAGATGGCTA
  841 CATCCATTAC GGGCAGACTG TCAAGCTTGT GTGCTCAGTG ACTGGCATGG CACTCCCAAG
  901 ATTGATAATT AGGAAAGTTG ATAAGCAGAC GGCATTACTG GATGCAGACG ACCCTGTATC
  961 ACAACTCCAC AAATGTGCAT TTTACCTTAA GGATACAGAA AGAATGTACT TGTGCCCTTC
 1021 TCAGAAGATA ATCCCAATTT CAGCCACTCC ATGTCCAAAG ACAAATAAGG AAATGATAAA
 1081 CGATGGAGCT TCCTGGACAT CATAGCAAGG ACCAGCAGAA TACACGTTTC TTATTGAGGA
1141 ATGGGCCCCG GATCCCTGTG GC
//
Primer: RDB12301_A3Jmb.seq
>03021_12301_A3Jm_pAxEFR2_A01_01.ab1
    1 CGGGAAAAAA TGGCTTTATT TGGTGAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA
   61 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG
  121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT
  181 ATGATCTCTA GTCAAGGCAC TATACATCAA ATATTTTTCC ATAATTTTCT TGTATAGCAG
  241 TGCAGCTTTT TCCTTTGTGG TGTAAATAGC AAAGCAAGCA AGAGTTCTAT TACTAAACAC
  301 AGCATGACTC AAAAAACTTA GCAATTCTGA AGGAAAGTCC TTGGGGTCTT CTACCTTTCT
  361 CTTCTTTTTT GGAGGAGTAG AATGTTGAGA GTCAGCAGTA GCCTCATCAT CACTAGATGG
  421 CATTTCTTCT GAGCAAAACA GGTTTTCCTC ATTAAAGGCA TTCCACCACT GCTCCCATTC
  481 ATCAGTTCCA TAGGTTGGAA TCTAAAATAC ACAAACAATT AGAATCAGTA GTTTAACACA
  541 TTATACACTT AAAAATTTTA TATTTACCTT AGAGCTTTAA ATCTCTGTAG GTAGTTTGTC
  601 CAATTATGTC ACACCACAGA AGTAAGGTTC CTTCACAAAG ATCCTCTAGC TACCTAGCTA
  661 GCTGGCCAGG ATCCCGTCGA CGGATCCCCA ACTTTTTGCT GCATTCAAGT CAGTTTCAGT
  721 CCCAGCAAAA AGACGGTAGT TAGGACACCA CGGTTGCTGT GGACGATGTG ACACTGGTAG
  781 AATTTGTGCT GGCATTTGTG TAATTCCCCT CGCTGTTTGT GTTTGACTCA TTGGAGGGCA
  841 CTTGGCTGGA GTTGGCTCTG AGAATCGCTC CTGCAGCGCT GCAGTGTGGC CTCGGCCCTG
  901 GCTCTGGTGT GTAGGTGAAG GTAAGGCTGG TGGAGTAAAT GACCCCGTCA TTTCGGACCA
  961 AAGTCACTGG AACCTGGACT GGCTGGCGGA CCCATCTCCA ACCCTTCCCG GAATGCAGAA
 1021 ATGTCTGGGA CCACACAGAG CATGCTCTCT CACATCTGTA CATTGTTTCG GCTTCTACAT
 1081 CCCCAACCAC ACTCGTAATT GGAGTAAAGT TTTGTCCTGT AAGTCCAGGC ATGGCCTACG
1141 TTCCCCCGCC GCCATTCACT GAAAGACTTT TCTTACCGAA CACA
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file Map RDB03021.gif from Depositor
Featured content EXR0009j (in Japanese)
Featured content EXR0030j (in Japanese)
Featured content EXR0042e (in English)
Featured content EXR0042j (in Japanese)
original - reference Kuroda, K., Delta-induced Notch signaling mediated by RBP-J inhibits MyoD expression and myogenesis. J. Biol. Chem., 274, 7238-7244 (1999). PMID 10066785.
user report - reference Sakamoto K., Reduction of NOTCH1 expression pertains to maturation abnormalities of keratinocytes in squamous neoplasms. Lab. Invest., 92 (5): 688-702 (2012). PMID 22330335.
user report - reference O'Brien, L.L., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Dev. Biol., 358 (2): 318-330 (2011). PMID 21871448.
user report - reference Lee, Y.J., Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells. Stem Cells and Cloning: Advances and Applications, 3: 165-173 (2010). PMID 24198522.
user report - reference Sakamoto K., Zfp64 participates in Notch signaling and regulates differentiation in mesenchymal cells. J. Cell Sci., 121, 1613-1623 (2008). PMID 18430783.
user report - reference Sakamoto K., Distinct roles of EGF repeats for the Notch signaling system. Exp. Cell Res., 302, 281-291 (2005). PMID 15561108.

2016.09.14

GNP_filter3_RDBDEP_html_160913.pl