Resource data sheet


Expressing luciferase under the control of CAG promoter

Catalog number RDB02443 (link to web catalog)
Resource name pCAcc-Luc+
Clone info. Expression vector of luciferase gene (delived from 7LvWF) under the control of CAG promoter. DNA sequence is available.

[Data sheet:]
Vector backbone pCAcc
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Classification Plasmid
Type_of_insert fragment firefly (Photinus pyralis) genomic DNA ()
Deposotor's page Hamada, Hirofumi | Nakamura, Takafumi |
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
(In Japanese [open/close])
Fee (non-profit org.)

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file Sequence RDB02443z.seq from Depositor
Electronic file Map RDB02443.gif from Depositor
Featured content Control Vectors (in English)
user report - reference Kato, T., Plasmid DNA delivery by arginine-rich cell-penetrating peptides containing unnatural amino acids. Bioorg. Med. Chem. pii: S0968-0896 (16) 30275-9 (2016). PMID 27132868.
user report - reference Oba, M., Plasmid DNA delivery using fluorescein-labeled arginine-rich peptides. Bioorg. Med. Chem. 23 (15): 4911-4918 (2015). PMID 26048025.
user report - reference Ge, Z, Targeted gene delivery by polyplex micelles with crowded PEG palisade and cRGD moiety for systemic treatment of pancreatic tumors. Biomaterials, 35 (10): 3416-3426 (2014). PMID 24439417.
user report - reference Dirisala, A., Optimized rod length of polyplex micelles for maximizing transfection efficiency and their performance in systemic gene therapy against stroma-rich pancreatic tumors. Biomaterials, 35 (20): 5359-5368 (2014). PMID 24720877.
user report - reference Chen Q., Homo-catiomer integration into PEGylated polyplex micelle from block-catiomer for systemic anti-angiogenic gene therapy for fibrotic pancreatic tumors. Biomaterials, 33 (18): 4722-4730 (2012). PMID 22444644.
user report - reference Kataoka, K., Charge-conversion ternary polyplex with endosome disruption moiety: a technique for efficient and safe gene delivery. Angew. Chem. Int. Ed. Engl. 47 (28): 5163-5166 (2008). PMID 18528828.
user report - reference Mickler, F.M., Effect of integrin targeting and PEG shielding on polyplex micelle internalization studied by live-cell imaging. J. Control Release, 156 (3): 364-373 (2011). PMID 21843561.
user report - reference Vachutinsky Y., Antiangiogenic gene therapy of experimental pancreatic tumor by sFlt-1 plasmid DNA carried by RGD-modified crosslinked polyplex micelles. J. Control. Release (2010). PMID 20138936.
user report - reference Oba M, Antiangiogenic gene therapy of solid tumor by systemic injection of polyplex micelles loading plasmid DNA encoding soluble flt-1. Mol. Pharm. 7 (2), 501-509 (2010). PMID 20178335.
user report - reference Oba M., Polyplex micelles prepared from omega-cholesteryl PEG-polycation block copolymers for systemic gene delivery. Biomaterials, 32 (2): 652-663 (2010) PMID 20932567.
user report - reference Oba M., Polyplex micelles with cyclic RGD peptide ligands and disulfide cross-links directing to the enhanced transfection via controlled intracellular trafficking. Mol. Pharm., 5, 1080-1092 (2008). PMID 19434856.
user report - reference Oba M., Cyclic RGD peptide-conjugated polyplex micelles as a targetable gene delivery system directed to cells possessing alphavbeta3 and alphavbeta5 integrins. Bioconjug. Chem., 18, 1415-1423 (2007). PMID 17595054.
user report - reference Nishiyama N., Light-induced gene transfer from packaged DNA enveloped in a dendrimeric photosensitizer. Nat. Mater., 4, 934-941 (2005). PMID 16299510.