Resource data sheet
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pxCANCre

A plasmid expressing the recombinase Cre tagged with a nls

Catalog number RDB01675 (link to web catalog)
Resource name pxCANCre
Clone info. A plasmid expressing the recombinase Cre tagged with a nuclear localization signal under the control of CAG promoter

[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB01675]
Vector backbone pxCAwt (deleted from pAxCAwt with Sal I)
Size of vector backbone 6,565 bp
Selectable markers Amp^r
Classification Plasmid
Type_of_insert fragment P1 phage cDNA ()
Deposotor's page Saito, Izumu
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions for distribution This material is provided with written consent of Depositor.
(In Japanese [open/close])
Remarks ((Additional form)) Approval Form(FormD)
(In Japanese [open/close])
Fee (non-profit org.)

We verified.

Data are summerized on test sheet below.

Test sheet RDB14151_A6Ebp1.pdf

Nucleotide sequence of a portion of this resource.

Primer: pAxCA_F1 RDB14151_A6Eba.seq
>01675_14151_A6Eb_3_pAxCA_F1_C08_08.ab1
    1 TAAGCTATCA TCTGCTACAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG
   61 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT GATTTATCGA TTTGCAGACC
  121 GTGCATCATG AGCGGCCCTC CAAAAAAGAA GAGAAAGGTA GAAGACCCGG GCGGCCGCAT
  181 GTCCAATTTA CTGACCGTAC ACCAAAATTT GCCTGCATTA CCGGTCGATG CAACGAGTGA
  241 TGAGGTTCGC AAGAACCTGA TGGACATGTT CAGGGATCGC CAGGCGTTTT CTGAGCATAC
  301 CTGGAAAATG CTTCTGTCCG TTTGCCGGTC GTGGGCGGCA TGGTGCAAGT TGAATAACCG
  361 GAAATGGTTT CCCGCAGAAC CTGAAGATGT TCGCGATTAT CTTCTATATC TTCAGGCGCG
  421 CGGTCTGGCA GTAAAAACTA TCCAGCAACA TTTGGGCCAG CTAAACATGC TTCATCGTCG
  481 GTCCGGGCTG CCACGACCAA GTGACAGCAA TGCTGTTTCA CTGGTTATGC GGCGGATCCG
  541 AAAAGAAAAC GTTGATGCCG GTGAACGTGC AAAACAGGCT CTAGCGTTCG AACGCACTGA
  601 TTTCGACCAG GTTCGTTCAC TCATGGAAAA TAGCGATCGC TGCCAGGATA TACGTAATCT
  661 GGCATTTCTG GGGATTGCTT ATAACACCCT GTTACGTATA GCCGAAATTG CCAGGATCAG
  721 GGTTAAAGAT ATCTCACGTA CTGACGGTGG GAGAATGTTA ATCCATATTG GCAGAACGAA
  781 CACGCTGGTT AGCACCGCAG GTGTAGAGAA GGCACTTAGC CTGGGGGTAA CTAAACTGGT
  841 CGAGCGATGG ATTTCCGTCT CTGGTGTAGC TGATGATCCG AATAACTACC TGTTTTGCCG
  901 GGTCAGAAAA AAATGGTGTT GCCGCGCCAT CTGCCCACCA GCCAGCTATC AAACTCGCGC
  961 CCTGGAAAGG ATTTTTTTGA AGCAACCTCA TCGATTGAAT TTTACGGCGC TACGATGGAC
 1021 TCTGGGTTCA GAGGATACCT TGGTCCTGAT CTGACACAGT GGTCCGATGA TCCGGAGCCG
1081 GCGCCGA
//
Primer: pAxCA_R1 RDB14151_A6Ebb.seq
>01675_14151_A6Eb_3_pAxCA_R1_F08_17.ab1
    1 GGACATGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG
   61 AATTGATTAT CGATTCTAGA CTAGTTTAAT TAATTTCTAG AGCGCTTAAT GGCTAATCGC
  121 CATCTTCCAG CAGGCGCACC ATTGCCCCTG TTTCACTATC CAGGTTACGG ATATAGTTCA
  181 TGACAATATT TACATTGGTC CAGCCACCAG CTTGCATGAT CTCCGGTATT GAAACTCCAG
  241 CGCGGGCCAT ATCTCGCGCG GCTCCGACAC GGGCACTGTG TCCAGACCAG GCCAGGTATC
  301 TCTGACCAGA GTCATCCTTA GCGCCGTAAA TCAATCGATG AGTTGCTTCA AAAATCCCTT
  361 CCAGGGCGCG AGTTGATAGC TGGCTGGTGG CAGATGGCGC GGCAACACCA TTTTTTCTGA
  421 CCCGGCAAAA CAGGTAGTTA TTCGGATCAT CAGCTACACC AGAGACGGAA ATCCATCGCT
  481 CGACCAGTTT AGTTACCCCC AGGCTAAGTG CCTTCTCTAC ACCTGCGGTG CTAACCAGCG
  541 TTTTCGTTCT GCCAATATGG ATTAACATTC TCCCACCGTC AGTACGTGAG ATATCTTTAA
  601 CCCTGATCCT GGCAATTTCG GCTATACGTA ACAGGGTGTT ATAAGCAATC CCCAGAAATG
  661 CCAGATTACG TATATCCTGG CAGCGATCGC TATTTTCCAT GAGTGAACGA ACCTGGTCGA
  721 AATCAGTGCG TTCGAACGCT AGAGCCTGTT TTGCACGTTC ACCGGCATCA ACGTTTTTCT
  781 TTTCGGATCC GCCGCATAAC CAGTGAAACA GCATTGCTGT CACTTGGTCG TGGCAGCCCG
  841 GACCGACGAT GAAGCATGTT TAGCTGGCCC AAATGTTGCT GGATAGTTTT TACTGCCAGA
  901 CCGCGCGCCT GAAGATATAG AAGATAATCG CGAACATCTT CAGGTTTCTG CGGGAAACCC
  961 ATTTTCCGGT TATTCACTTT GCACCATGAC GCCCACGAAC GGCCAACCGG ACAGAAGGCA
1021 TTTTGCAGCT ATGCTCAGAA ACGGCTTACG ATGCCATGCA AGCATGATCC AATTC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Featured content EXR0045e (in English)
Featured content EXR0045j (in Japanese)
Featured content Control Vectors (in English)
Featured content Cre-loxP and FLP-FRT System (in English)
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user report - reference Shimada H., Introduction of tau mutation into cultured Rat1-R12 cells by gene targeting, using recombinant adeno-associated virus vector. Cell. Mol. Neurobiol., 29, 699-705 (2009). PMID 19305950.
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2016.07.26

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