Resource data sheet
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pco12 EGFR

Human EGF receptor (EGFR) cDNA

Catalog number RDB01276
Resource name pco12 EGFR
Alternative name Human EGF receptor cDNA
Clone info. Human EGF receptor (EGFR) cDNA

[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB01276]
Vector backbone pCO12 (Plasmid)
Size of vector backbone 11.2 kb
Selectable markers Amp^r
Gene/insert name Human EGFR cDNA
Other clones with this gene human EGFR (NCBI Gene 1956) |
Deposotor's page Yamamoto, Tadashi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01276 pco12 EGFR DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14268_A6Grp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCO12_F RDB14268_A6Gra.seq
>01276_14268_A6Gr_pCO12_F_G06_21.ab1
    1 ACACCCTATT GGATTGGGTC CCAGCTCTGA AAAAAGGAAA GAGAAACAAA AACAAAAAAC
   61 ATATAGTGTT TTATCTGTGC TTATGCCCGC AGCCCGAGCC GCACCCGCCG CGGATCCGCT
  121 AGAGTCGATG GCCCCGGCGG CCGCCGCCGC CCAGACCGGA CGACAGGCCA CCTCGTCGGC
  181 GTCCGCCCGA GTCCCCGCCT CGCCGCCAAC GCCACAACCA CCGCGCACGG CCCCCTGACT
  241 CCGTCCAGTA TTGATCGGGA GAGCCGGAGC GAGCTCTTCG GGGAGCAGCG ATGCGACCCT
  301 CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG GCGAGTCGGG
  361 CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG TTGGGCACTT
  421 TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG GTCCTTGGGA
  481 ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG ACCATCCAGG
  541 AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT TTGGAAAACC
  601 TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA GTCTTATCTA
  661 ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA CAGGAAATCC
  721 TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG AGCATCCAGT
  781 GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC CAGAACCACC
  841 TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GGAGCTGCTG GGGGTGCAAG
  901 GAGAGGAGAA CTGCCCAGAA ACTGACCAAA ATTCATCCTG GTGCCCAGCA GTGCTTCCCG
  961 GGGCGCTGCC GTGGGCAAGT CCCCCCCAAG TGAACTTGCT GCCACAACCA GTGTGCTGCA
 1021 AGCTGCACAG GCCCCCGGGG AGAGCGACTG CCCTGGATCT GCCGGCAATC CGAGACGAAG
1081 ACCACCGATG GCCAAGGACA CAACT
//
Primer: pCR12_R RDB14268_A6Grb.seq
>01276_14268_A6Gr_pCO12_R_H06_24.ab1
    1 CAGCCATTCC CGGGTCCTTG AGGCCCTGTG CCGGGACGGG CACAAAAGGA CATGACTGAC
   61 CCTCGAAGAC ATGGGCCTGC AGGTCGATGG AAAGATAAAT TTCTGCTCAA AGGGACAATA
  121 TTCTTGCTGG ATGCGTTTCT GTAAATGCTT CACAGTTTGA AGACAAAGGA ATGCAACTTC
  181 CCAAAATGTG CCCGAGGTGG AAGTACTTCC TGGCTAGTCG GTGTAAACGT TGCAAAACCA
  241 GTCTGTGGGT CTAAGAGCTA ATGCGGGCAT GGCTGTTGGG ATGGAGGACC TGCTGTGGCT
  301 TGGTCCTGGG TATCGAAAGA GTCTGGATTT TTAGGGCTCA TACTATCCTC CGTGGTCATG
  361 CTCCAATAAA TTCACTGCTT TGTGGCGCGA CCCTTAGGTA TTCTGCATTT TCAGCTGTGG
  421 AGCCCTTAAA GATGCCATTT GGCTTGGCTT CCTTGGGAAA GAAGTCCTGC TGGTAGTCAG
  481 GGTTGTCCAG GCTAATTTGG TGGCTGCCTT TCTGGGCCCA GTGGGCAGGG CTGTCGAATG
  541 TGCTGTTGAC ACAGGTGGGC TGGACAGTGT TGAGATACTC GGGGTTGCCC ACTGCAGTGC
  601 TGTGGGGGTC CTGGTAGTGT GGGTCTCTGC TGGGCGCGGG GTTCAGAGGC TGATTGTGAT
  661 AGACAGGATT CTGCACAGAG CCAGCGGGCC TTTTGGGAAC GGACTGGTTT ATGTATTCAG
  721 GCACTGGGGA GGAAGGTGTC GTCTATGCTG TCCTCAGTCA AGGCGCCTGT GGGGTCTGAG
  781 CTGTATCGCT GCAAGAAGCT GTCTTTCCTT GATGGGACAG CTTTGCAGCC CATTTCTATC
  841 AATGCAAGCC ACGGGTGGAA ATTGTTGCTG GTTGCACTCA GAGAGCTCAG GAGGGGAGTC
  901 CGTGACGTGG AAGGGGCTGC TGAAGAAGCC CTGCTGTGGG GATGAGTACT CGTCGGCATC
  961 CACCACGTCG TCCATGTTCT TCTTTCATTC CAATCATGGC ACGGTAAGAA GTTTGGAGTC
 1021 CTGGTAGACT ATGCAAAATG CAATTCCTTC CATCCCCCCT GAATTGACAG GGTACGCTTG
1081 GGGGTCTCGG GCCAATTTTG GGCA
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Link to old catalog RDB01276 (link)
Electronic file Map RDB01276.gif from Depositor
Electronic file PDF RDB01276.pdf from Depositor

Featured content

Featured content Dnaconda's recommendation EXR0034e (English text)
Featured content Dnaconda's recommendation EXR0034j (Japanese text)

Reference

original Velu, T.J., Retroviruses expressing different levels of the normal epidermal growth factor receptor: biological properties and new bioassay. J. Cell Biochem., 39, 153-166 (1989). PMID 2565908.
original Velu, T.J., Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene. Science, 238, 1408-1410 (1987). PMID 3500513.
others Clark, A.J., Synthesis of epidermal growth factor (EGF) receptor in vitro using SP6 RNA polymerase-transcribed template mRNA. Biochim. Biophys. Acta., 867, 244-251 (1986). PMID 3017432.
user report Fukuda, N., Rapid Evaluation of Tyrosine Kinase Activity of Membrane-Integrated Human Epidermal Growth Factor Receptor Using the Yeast G gamma Recruitment System. ACS Synth. Biol. 4 (4): 421-429 (2015). PMID 25006755.
user report Kojima, K., ESCRT-0 Protein Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate (Hrs) Is Targeted to Endosomes Independently of Signal-transducing Adaptor Molecule (STAM) and the Complex Formation with STAM Promotes Its Endosomal Dissociation. J. Biol. Chem. 289 (48): 33296-33310 (2014). PMID 25296754.
user report Hiragun, T., Surface plasmon resonance-biosensor detects the diversity of responses against epidermal growth factor in various carcinoma cell lines. Biosens. Bioelectron., 32 (1): 202-207 (2012). PMID 22204782.
user report Tanaka, K., Selection and growth regulation of genetically modified cells with hapten-specific antibody/receptor tyrosine kinase chimera. Biotechnol. Progr., 25, 1138-1145 (2009). PMID 19569211.
user report Dyson M.R., Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression BMC Biotechnol., 4, 1-18 (2004). PMID 15598350.
user report Niikura T., Insulin-like growth factor I (IGF-I) protects cells from apoptosis by Alzheimer's V642I mutant amyloid precursor protein through IGF-I receptor in an IGF-binding protein-sensitive manner. J. Neurosci., 21, 1902-1910 (2001). PMID 11245675.
user report Fujino T., Enhanced expression of heparin-binding EGF-like growth factor and its receptor in hypertrophied left ventricle of spontaneously hypertensive rats. Cardiovasc. Res., 38, 365-374 (1998). PMID 9709397.
user report Nakamura N., An epidermal growth factor receptor/Jak2 tyrosine kinase domain chimera induces tyrosine phosphorylation of Stat5 and transduces a growth signal in hematopoietic cells. J. Biol. Chem., 271, 19483-19488 (1996). PMID 8702638.

2017.04.07

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