Resource data sheet
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pSV2 erb B2

Human erb B2 cDNA expression vector

Catalog number RDB01273
Resource name pSV2 erb B2
Alternative name Human erb B2 cDNA expression vector
Clone info. Human erb B2 cDNA driven by SV40 promoter

[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB01273]
Vector backbone pSV2 (Plasmid)
Selectable markers Amp^r
Gene/insert name Human c-erbB2/erb-B cDNA
Depositor Yamamoto, Tadashi |
 
Other clones in our bank human ERBB2 (NCBI Gene 2064) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01273 pSV2 erb B2 DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13019_A4Kh.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse RDB13019_A4Kha.seq
>01273_13019_A4Kh_SV40pA3'ElementReverse_B05_05.ab1
    1 GAGTTCCTGG GGGGCGGGAC CTATGGAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
   61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
  121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
  181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA
  241 TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC
  301 ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA
  361 CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC
  421 AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG
  481 AAACGCGCGA GGCAGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC
  541 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG
  601 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC
  661 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT
  721 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT
  781 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGTC TAGGCTTTTG CAAAAAGCTT
  841 GGGGCAGCCG CGCGCCCCTT CCCACGGGGC CCTTTACTGC GCCGCGCGCC CGGCCCCCAC
  901 CCCTCGCAGC ACCCCGCGCC CCGCGCCCTC CCAGCCGGGT CCAGCCGGAG CCATGGGGGC
  961 CGGAGCCGCA GTGAGCACCA TGGAGCTGGC GGGCCTTGTG CCGCTGGGGG CTCCTCCTCG
 1021 CCCTCTTGCC CCCCGGGAGC CGCGAGCACC CAAGTGTGCA CCGGCACAGA CCATGAAAGC
 1081 TGCGGCTCCC TGCCAGTCCC GAGACCCAAC TTGACCATGC CTCCGGCCAA CCTCTACCAG
1141 GGATCTTCCC AAGGTGG
//
Primer: SV40-3'UTR RDB13019_A4Khb.seq
>01273_13019_A4Kh_SV40-3'UTR_A05_02.ab1
    1 TCAACAGCAA GGTAGGTTCC TTCACAAAGA TCCAAGCTTG CAAGCTTGAA AAAAACAAAA
   61 CAAAAAAAGT AAAAACTAAA CAGAAAAGCA CTCTGTACAA AGCCTGGATA CTGACACCAT
  121 TGCTGTTCCT TCCTCATGGG GGGCAGTACT AGGTTTCAGG GACAGTCTCT GAATGGGTCG
  181 CTTTTGTTCT TAGACACTCC CTTAGGGCCG CTTCCCCTCT CAGGCCAGCT TCCCTAAGGC
  241 TTTCAGTACC CAGGATCTGG AAGGAAAGGG CCAAGCTGGA CTGTGGCATC CACTGGACCC
  301 TAGAGTCTCA TTGGGCAGGG CCTCAGAATC CACAAAGACT CCCCAGTGCT GTTCCTCTTC
  361 CAACGAGGCT GGACCCCTTC CAGCCATCTG GGAACTCAAG CAGGAAGGAA GGTTCCTTAG
  421 GACAGGTTCC TGGCATGGCA GGTTCCCCTG GAAGTGGTCG GAGGGCCCTC CCACCTCTTG
  481 ATGCCAGCAG AAGTCAGGCC TTCCCTGCTC CCTGAGGACA CATCAGGGCT TCTGCGGACT
  541 TGGCCTTCTG GTTCACACTG GCACGTCCAG ACCCAGGTAC TCTGGGTTCT CTGCCGTAGG
  601 TGTCCCTTTG AAGGTGCTGG GTGGAGCCCC CCGCTCTGGT GGGTCCTGGT CCCAGTAATA
  661 GAGGTTGTCG AAGGCTGGGC TGAAGGCAGG AGGAGGGTGG GGCTGAGGGG CAGCTCCTCC
  721 CTGGGGTGTC AAGTACTCGG GGTTCTCCAC GGCACCCCCA AAGGCAAAAA CGTCTTTGAC
  781 GACCCCATTC TTCCCTGGGG AGAGAGTCTT GGGCCTTTCC AGAGTGGCAC CAGCAGGTCG
  841 GGCAGCAGGC AGAGGGCCCT CTCGGGGCGA AGGGGGCTGG GGCCGAACAT CTGGCTGGTT
  901 CACATATTCA GGCTGGGGGC TGCAGGTCAG GGGGGCAACG TAGCCATCAG TCTCAGAGGG
  961 CAGGGGTACT GTGGGTCCTC ACTGTACCGC TGTAGAGGCT GGGGTCATGT GTGGGGAGGC
 1021 TTTGCAGCCC CTTGGCTGCC CCATTCCCAG GTCACCATCA ATACATCGGA GCCAGCCCCT
 1081 TTCGGAGGTG CCAGTGAGAC CTGGGGCCTC CTCACAGAGG GCTCAGCCTA GTGTCAGGTT
1141 CCCCACGACC ACTCCTTGGT TAGAAATGAA GCCTTGTCCG
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Link to old catalog RDB01273 (link)
Electronic file Map RDB01273.gif from Depositor

Featured content

Reference

original Masuko, T., A murine monoclonal antibody that recognizes an extracellular domain of the human c-erbB-2 protooncogene product. Jpn. J. Cancer Res. 80 (1): 10-14 (1989).original PMID 2565325.
others Matsuda, S., 17 beta-estradiol mimics ligand activity of the c-erbB2 protooncogene product. Proc. Natl. Acad. Sci. U.S.A. 90 (22): 10803-10807 (1993). PMID 7902571.
others Ullrich, A., Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. Nature 319, 230-234 (1986). PMID 6328312.
user report Miura, T., Ligand-inducible dimeric antibody for selecting antibodies against a membrane protein based on mammalian cell proliferation. Biotechnol. Bioeng. 113 (5): 1113-1123 (2016). PMID 26479395.
user report ,

2017.05.15

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