Resource data sheet
Prev.

pTK5

HSV I thymidine kinase

Catalog number RDB01027 (link to web catalog)
Resource name pTK5
Alternative name HSV I thymidine kinase
Clone info. Herpes simplex virus type I thymidine kinase
At least covering 46589..47442 and 47550..47719 of GU734771.1. Reference for insert sequence, Wigler, M., Cell, 14 (3): 725-731 (1978).
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB01027]
Vector backbone pBR322
Size of vector backbone 4,361 bp
Selectable markers Amp^r
Classification Plasmid
Type_of_insert fragment HSV Genomic DNA ()
Deposotor's page Soeda, Eiichi
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
(In Japanese [open/close])
Fee (non-profit org.)

We verified.

Data are summerized on test sheet below.

Test sheet RDB12799_A4F2.pdf

Nucleotide sequence of a portion of this resource.

Primer: pBR322_BamHI_F RDB12799_A4F2a.seq
>01027_12799_A4F2_pBR322_BamHI_F_B10_04.ab1
    1 GAAGGTTCTG TGGGATCAAT GAGTCTTCGG ACCTCGCGGG GGCCGCTTAA GCGGTGGTTA
   61 GGGTTTGTCT GACGCGGGGG GAGGGGGAAG GAACGAAACA CTCTCATTCG GAGGCGGCTC
  121 GGGGTTTGGT CTTGGTGGCC ACGGGCACGC AGAAGAGCGC CGCGATCCTC TTAAGCACCC
  181 CCCCGCCCTC CGTGGAGGCG GGGGTTTGGT CGGCGGGTGG TAACTGGCGG GCCGCTGACT
  241 CGGGCGGGTC GCGCGCCCCA GAGTGTGACC TTTTCGGTCT GCTCGCAGAC CCCCGGGCGG
  301 CGCCGCCGCG GCGGCGACGG GCTCGCTGGG TCCTAGGCTC CATGGGGACC GTATACGTGG
  361 ACAGGCTCTG GAGCATCCGC ACGACTGCGG TGATATTACC GGAGACCTTC TGCGGGACGA
  421 GCCGGGTCAC GCGGCTGACG CGGAGCGTCC GTTGGGCGAC AAACACCAGG ACGGGGCACA
  481 GGTACACTAT CTTGTCACCC GGAGGCGCGA GGGACTGCAG GAGCTTCAGG GAGTGGCGCA
  541 GCTGCTTCAT CCCCGTGGCC CGTTGCTCGC GTTTGCTGGC GGTGTCCCCG GAAGAAATAT
  601 ATTTGCATGT CTTTAGTTCT ATGATGACAC AAACCCCGCC CAGCGTCTTG TCATTGGCGA
  661 ATTCGAACAC GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG CATATTAAGG
  721 TGACGCGTGT GGCCTCGAAC ACCGAGCGAC CCTGCAGCGA CCCGCTTAAC AGCGTCAACA
  781 GCGTGCCGCA GATCTTGGTG GCGTGAAACT CCCGCACCTC TTCGGCAAGC GCCTTGTAGA
  841 AGCGCGTATG GCTTCGTACC CCTGCCATCA ACACGCGTCT GCGTTCGACC AGGCTGCGCG
  901 TTCTCGCGGC CATAGCAACC GACGTACGGC GTTGCGCCCT CGCCGGCAGC AAGAAGCCAC
  961 GGAAGTCCGC CTGGAGCAGA AAATGCCCAC GCTACTGCGG GTTTATATAG ACGGTCCCTC
 1021 ACGGGATGGG GGAAAACCCA CCCACCACGC AACTGCTGGG TGGGCCCTGG GGCTCGCGCG
 1081 ACGATATCGT CTACGTTACC CGAGCCGAAT GACTTACTGC CAAGTGCCTG GGGGCATCTC
1141 GGACATCGCG ACATTCTTAT ACAATCCTC
//
Primer: pBR322_BamHI_R RDB12799_A4F2b.seq
>01027_12799_A4F2_pBR322_BamHI_R_C10_07.ab1
    1 GCGTTGAGAA ACACAGAATC CGCGGCACAG CCCGCGGCCC CGCGGGCGGC CAACCCGGCA
   61 AGCGCGCGCG AGTGGGCCAA AAAGCCTAGC AGGTCGGAGA GGCAGACCGC GCCGTTTGCG
  121 TGGGCGGCGT TCACGAAAGC AAAACCCGAC GTCGCGAGCA GCCCCGTTAG GCGCCAGAAG
  181 AGAGGGGGGC GCGGGCCCTG CTCGGCGCCC GCGTCCCCCG AGAAAAACTC CGCGTATGCC
  241 CGCGACAGGA ACTGGGCGTA GTTCGTGCCC TCCTCCGGGT AGCCGCCCAC GCGGCGGAGG
  301 GCGTCCAGCG CGGAGCCGTT GTCGGCCCGC GTCAGGGACC CTAGGACAAA GACCCGATAC
  361 CGGGGGCCGC CCGGGGGCCC GGGAAGAGCC CCCGGGGGGT TTTCGTCCGC GGGGTCCCCG
  421 ACCCGATCTA GCGTCTGGCC CGCGGGGACC ACCATCACTT CCACCGGAGG GCTGTCGTGC
  481 ATGGATATCA CGAGCCCCAT GAATTCCCGC CCGTAGCGCG CGCGCACCAG CGCGGCATCG
  541 CACCCGAGCA CCAGCTCCCC CGTCGTCCAG ATGCCCACGG GCCACGTCGA GGCCGACGGG
  601 GAGAAATACA CGTACCTACC TGGGGATCTC AACAGGCCCC GGGTGGCCAA CCAGGTCGTG
  661 GACGCGTTGT GCAGGTGCGT GATGTCCAGC TCCGTCGTCG GGTGCCGCCG GGCCCCAACC
  721 GGCGGTCGGG GGGGCGGTGT ATCACGCGGC CCCGCTTGGG TGGCTCGCCC GTCGCCACGT
  781 TTGTCTCCCC GCGGGGAACG TCAGGGCCCT CGGGTTCAGG GACGGCCGAA AACGTTACCC
  841 AGGCCCGGGA ACGGCAACTA CACGGAAGCG ACTGGATTTG TACAATAGAC CCCTTAAGGG
  901 GGCCGACCGA AGGGGGGGAG GGCTGGGCCG GTTCGGCTCG ACCGTGGTCG GGGGCGGGCA
  961 GGCTCGCCGT TTCCGGGGGC CCGGGCTGAA GCAGCGTAAG GTCCTTCGGG CACTGTAAAA
1021 GGAA
//
Primer: HSV_TKpA_R RDB12799_A4F2c.seq
>01027_12799_A4F2_HSV_TKpA_R_C10_07.ab1
    1 ACGGGGTTCT TCCGGGTATT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCATCTCCCG
   61 GGCAAACGTG CGCGTCTCAG GTCGCAGATC GTCGGTATGG AGCCGGGGGT GGTGACGTGG
  121 GTCTGGACCA TCCCGGAGGT AAGTTGCAGC AGGGCGTCCC GGCAGCCGGC GGGCGATTGG
  181 TCGTAATCCA GGATAAAGAC GTGCATGGGA CGGAGGCGTT TGGCCAAGAC GTCCAAGGCC
  241 CAGGCAAACA CGTTGTACAG GTCGCCGTTG GGGGCCAGCA ACTCGGGGGC CCGAAACAGG
  301 GTAAATAACG TGTCCCCGAT ATGGGGTCGT GGGCCCGCGT TGCTCTGGGG CTCGGCACCC
  361 TGGGGCGGCA CGGCCGTCCC CGAAAGCTGT CCCCAATCCT CCCGCCACGA CCCGCCGCCC
  421 TGCAGATACC GCACCGTATT GGCAAGCAGC CCGTAAACGC GGCGAATCGC GGCCAGCATA
  481 GCCAGGTCAA GCCGCTCGCC GGGGCGCTGG CGTTTGGCCA GGCGGTCGAT GTGTCTGTCC
  541 TCCGGAAGGG CCCCCAACAC GATGTTTGTG CCGGGCAAGG TCGGCGGGAT GAGGGCCACG
  601 AACGCCAGCA CGGCCTGGGG GGTCATGCTG CCCATAAGGT ATCGCGCGGC CGGGTAGCAC
  661 AGGAGGGCGG CGATGGGATG GCGGTCGAAG ATGAGGGTGA GGGCCGGGGG CGGGGCATGT
  721 GAGCTCCCAG CCTCCCCCCC GATATGAGGA GCCAGAACGG CGTCGGTCAC GGCATAAGGC
  781 ATGCCCATTG TTATCTGGGC GCTTGTCATT ACCACCGCCG CGTCCCCGGC CGATATCTCA
  841 CCCTGGTCGA GGCGGTGTTG TGTGGTGTAG ATGTTCGCGA TTGTCTCGGA AGCCCCCCAG
  901 CACCTGCCAG TAAGTCATCG GCTCGGGGTA CGTAGACGAT ATCGTCGCGC GAACCCAGGG
  961 CCACCAGCAG TTGCGTGGTG GTGGTTTTCC CCATCCCGTG AGGACCGTTC TATATAAACC
 1021 CGCAGTTAGC GTGGGCAATT TTTCTGCTTC CAGGCGGACT TTCCCGTGGC CTTCCTTGCT
 1081 TGCCCGGGCC GAGGGGGCGC CAAACGGTCG TACGATCCGA TTGCCTTATG GTCGGCCGAG
1141 GAAACCGCCT GGCCAGAGCC CCT
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file Map RDB01027.gif from Depositor
Featured content EXR0010e (in English)
Featured content EXR0010j (in Japanese)
Featured content Suicide gene therapy (in English)
others - reference Wigler, M., Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor. Cell, 14 (3): 725-731 (1978).
user report - reference Kurayoshi, K., Cancer cell specific cytotoxic gene expression mediated by ARF tumor suppressor promoter constructs. Biochem. Biophys. Res. Commun., 450 (1): 240-246 (2014). PMID 24893334.

2016.07.26

GNP_filter3_RDBDEP_html_160610.pl