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Recombinant Virus Database

Empty vectors for construction of recombinant adenovirus


RDB no.Name of vectorShort titleClassification
917pAxcwA cassette cosmid for construction of recombinant adenovirusVector
918pAxcA cassette cosmid for construction of recombinant adenovirusVector
1678pAxCAwtA cassette cosmid for construction of a recombinant adenovirusVector
2564pAxCEAwtRCloning vector to generate recombinant adenovirus driven by human CEA promoterVector
2565pAxCEAwtCloning vector to generate recombinant adenovirus driven by human CEA promoterVector
3120pAxcwitA dual cassette for constructing recombinant adenovirusVector
3121pAxCAwtitA dual cassette for constructing recombinant adenovirus containing CAG promoterVector
3220pAF16RctCosmid clone of genomic fragment of adenovirus type 5Vector
3380pAxCAiLacZitShuttle vector to generate recombinant adenovirus harbouring E. coli LacZShuttleVector
3444pUAd5LShuttle vector to generate recombinant adenovirusVector
3445pUAd5LCAShuttle vector to generate recombinant adenovirusVector
3446pUAd5LCALNLShuttle vector to generate recombinant adenovirusVector
3447pUAd5LCAShuttle vector to generate recombinant adenovirusVector
3448pUAd5LCALNLShuttle vector to generate recombinant adenovirusVector
5212pAxcwit2A dual cassette for constructing recombinant adenovirusVector
5213pAxCAwtit2A dual cassette for constructing recombinant adenovirusVector
5214pAxCALNLwtit2A dual cassette for constructing recombinant adenovirusVector
5215pAxEFwtit2A dual cassette for constructing recombinant adenovirusVector


Name of vectorCloning sitePromoteraCre-loxPbProduction of recombinant adenovirusRDB no.Map
Intact-genome transfectioncTwo-cosmiddCOS-TPCeFiber-modified
COS-TPC
pAxcwSwaI    Yes 917 [ pdf ]
pAxCAwtSwaICA   Yes 1678 [ pdf ]
pAxCALNLwSwaICAYes  Yes f 
pAxcwitSwaI  Yes Yes 3120 [ pdf ]
pAxCAwtitSwaICA Yes Yes 3121 [ pdf ]
pUAd5LSwaI   YesYesYes3444 [ pdf ]
pUAd5LCASwaICA  YesYesYes3445 [ pdf ]
pUAd5LCALNLSwaICAYes YesYesYes3446 [ pdf ]
pAxCEAwtSwaICEA   Yes 2565 [ pdf ]
pAxcwit2SwaI  Yesg Yes 5212 [ pdf ]
pAxCAwtit2SwaICA Yesg Yes 5213 [ pdf ]
pAxCALNLwtit2SwaICAYesYesg Yes 5214 [ pdf ]
pAxEFwtit2SwaIEF-1 alpha Yesg Yes 5215 [ pdf ]

a, CA, a modified promoter of the chicken gene for beta-actin with a cytomegalovirus-immediate early enhancer (CMV-IE); CEA, carcinoembryonic antigen; EF-1 alpha, elongation factor 1 alpha.

b, Target gene harbored in the recombinant adenovirus can be expressed by co-infection with AxCANCre (RDB no. 1748, Gene 181: 207-212, 1996).

c, Fukuda et al., Microbiol. Immunol. 50: 643-654, 2006.

d, Use pAF16Rct (RDB no. 3220) together to generate rAd.

e, Miyake et al., Proc. Natl. Acad. Sci. USA 93: 1320-1324, 1996.

f, Available from Takara Bio Inc.

g, PacI restriction enzyme is also applicable for linearization as well as Csp45I restriction enzyme.


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2010.05.19