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News

A novel gene involved in germ cell formation commonly discovered in fruit fly and mice (Japanese text only) (2017.02.01 T.M.)

A genetic contamination test for genetically-modified mice at BRC has become available as a simple kit (Japanese text only) (2017.02.01 T.M.)

♦ Congratulations to Dr. Yoshinori Ohsumi!! (2016.10.03 T.M.)

  • The 2016 Nobel Prize in Physiology or Medicine is awarded to Dr. Yoshinori Ohsumi for his discoveries of mechanisms for autophagy.

♦ Plasmid clones to study autophagy are available. (2016.10.03 T.M.)

Dnaconda's Recommendation

A fluorescent probe to evaluate activity of autophagy. (2017/02/21 N.N.)

  • Plasmid clone of a novel probe capable of evaluating the activity of autophagy by GFP/RFP signal ratio has arrived.[more]

Green- and Red-emitting luciferases with luminescence. (2017/2/2 T.M.)

Resource information

♦ Histac-H3K9/K14 for visualization of acetylation activity of histone H3 in cells.(2016/9/27 T.M.)

  • Reference
    Nakaoka, S., Sasaki, K., Ito, A., Nakao, Y., Yoshida, M. A Genetically Encoded FRET Probe to Detect Intranucleosomal Histone H3K9 or H3K14 Acetylation Using BRD4, a BET Family Member. ACS Chem. Biol. 11 (3): 729-733, 2016. PMID: 25946208

♦ GimRET for visualization of protein concentration in cells.(2016/9/13 T.M.)

  • Reference
    Morikawa, J.T., Fujita, H., Kitamura, A., Horio, T., Yamamoto, J., Kinjo, M., Sasaki, A., Machiyama, H., Yoshizawa, K., Ichimura, T., Imada, K., Nagai, T., Watanabe, M.T. Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it. Sci. Rep. 6: 22342, 2016. PMID: 26956628

♦ Nakanori that specifically binds to a complex of sphingomyelin and cholesterol.(2016/9/13 T.M.)

  • Reference
    Makino, A. et al. A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB. J., 2016. Aug, 4. In press. PMID: 27492925.

♦ Fluorescent Ubiquitination-based Cell Cycle Indicator (Fucci) by single plasmid.(2016/6/20 T.M.)

  • Fucci2a cell cycle phase markers allow visualization and estimation of cell cycle progress by observation of green and red fluorescent proteins. [more...]

♦ Genius method to get rid of the target protein with human culture cells (2016.03.25 K.N.)

    Now available the plasmid for the system of Auxin-indusible degradation (AID) tag of your target protein in human cells from RIKEN BRC. [more...]

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Genetic resources

GNP
Searching plasmids by using KEGG

GNP
GNP Human Full-Length cDNA

B6N BAC
Searching BAC by the BAC browser

Empty Backbone for Mammalian Cell Experiment
Empty Backbone Vector

2017.02.21