RIKEN BRC DNA BANK (en)
BioResource Center
  Japanese
RIKEN BRC DNA BANK
Home   DNA Resources   How to Order   Deposit   Technical Notes   Information


Access counter started in 7/17/1997

dnaconda01

News

♦ We will be closing from May 3rd to May 5th due to national holidays. We will resume normal business from May 8th.

A novel gene involved in germ cell formation commonly discovered in fruit fly and mice (Japanese text only) (2017.02.01 T.M.)

A genetic contamination test for genetically-modified mice at BRC has become available as a simple kit (Japanese text only) (2017.02.01 T.M.)

♦ Congratulations to Dr. Yoshinori Ohsumi!! (2016.10.03 T.M.)

  • The 2016 Nobel Prize in Physiology or Medicine is awarded to Dr. Yoshinori Ohsumi for his discoveries of mechanisms for autophagy.

♦ Plasmid clones to study autophagy are available. (2016.10.03 T.M.)

Dnaconda's Recommendation

Establishment of deficient cells using Cre recombinase and floxed mouse derived cells (2017/06/23 T.M.)

  • Has2 deficient cells were established by using Cre recombinase expressing AxCANCre adenovirus. Mouse Has2 gene floxed allele cells were segregated from the transgenic mice having the exon 2 flanked by two loxP sites. And then the cells were infected with AxCANCre adenovirus carrying the Cre recombinase to remove the exon 2.[more]

M-INK, a novel tool for tagging the mature melanosomes.(2017/05/02 N.N.)

  • A novel tool "M-INK" to visualize melanosome in melanocyte and keratinocyte has arrived.[more]

G-CaMP, a fluorescent calcium sensor.(2017/03/16 N.N.)

  • A Fluorescent probes "G-CaMP" for measuring intracellular calcium concentration are available.[more]

Construction of reporter gene having long promoter by using BAC clone and with recombineering technique.(2017/02/28 T.M.)

  • Luciferase reporter assay is a powerful tool for real-time monitoring of gene expression in living cells. It is recommended to construct reporter gene with lager promoter but hard to do so by means of common cloning technique. In this article, 20 kb promoter of Hprt gene was retrieved and cloned into a luciferase reporter by using BAC clone and with recombineering technique.[more]

A fluorescent probe to evaluate activity of autophagy. (2017/02/21 N.N.)

  • Plasmid clone of a novel probe capable of evaluating the activity of autophagy by GFP/RFP signal ratio has arrived.[more]

Green- and Red-emitting luciferases with luminescence. (2017/2/2 T.M.)

Resource information

♦ Histac-H3K9/K14 for visualization of acetylation activity of histone H3 in cells.(2016/9/27 T.M.)

  • Reference
    Nakaoka, S., Sasaki, K., Ito, A., Nakao, Y., Yoshida, M. A Genetically Encoded FRET Probe to Detect Intranucleosomal Histone H3K9 or H3K14 Acetylation Using BRD4, a BET Family Member. ACS Chem. Biol. 11 (3): 729-733, 2016. PMID: 25946208

♦ GimRET for visualization of protein concentration in cells.(2016/9/13 T.M.)

  • Reference
    Morikawa, J.T., Fujita, H., Kitamura, A., Horio, T., Yamamoto, J., Kinjo, M., Sasaki, A., Machiyama, H., Yoshizawa, K., Ichimura, T., Imada, K., Nagai, T., Watanabe, M.T. Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it. Sci. Rep. 6: 22342, 2016. PMID: 26956628

♦ Nakanori that specifically binds to a complex of sphingomyelin and cholesterol.(2016/9/13 T.M.)

  • Reference
    Makino, A. et al. A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB. J., 2016. Aug, 4. In press. PMID: 27492925.

♦ Fluorescent Ubiquitination-based Cell Cycle Indicator (Fucci) by single plasmid.(2016/6/20 T.M.)

  • Fucci2a cell cycle phase markers allow visualization and estimation of cell cycle progress by observation of green and red fluorescent proteins. [more...]

♦ Genius method to get rid of the target protein with human culture cells (2016.03.25 K.N.)

    Now available the plasmid for the system of Auxin-indusible degradation (AID) tag of your target protein in human cells from RIKEN BRC. [more...]

Quality Examination and Information Release Policy on Our Bioresources (2014.09.01)

Announcements

Announcement of the interruption on our mail server (Mar 01, 2017)

Prepayment of distribution fees (overseas organizations only)

New Information from RIKEN BioResource Center

Full article..

 
Resources for Various Experiment
 
   
Gene Set Collection
 
 
Resources for
Cultured microbe
Cultured animal cell
Experimental animal
   in vitro experiment
 
Plant DNA Resources
   
 
 
 
 

RIKEN and all those who have deposited materials or information are not liable for any harm to any individual that might arise as a result of the use of any resources or informaion in the RIKEN databases anywhere in the world.

Caution when you use our bioresources





Genetic resources

GNP
Searching plasmids by using KEGG

GNP
GNP Human Full-Length cDNA

B6N BAC
Searching BAC by the BAC browser

Empty Backbone for Mammalian Cell Experiment
Empty Backbone Vector

2017.03.16