S. pombe ORFeome Clones

Clones & Clone set (ja)

Clones & Clone set (en)

Data Sheet

Vector Sequences

Links & Strains

Schizosaccharomyces pombe ORFeome clones were deposited by Dr. Minoru Yoshida, Chemical Genetics Lagoratory, RIKEN; These consists of 4,900 entry clones. Individual clones are available.

Press release
Localization data is available at

http://www.riken.jp/SPD/index.html

The Schizosaccharomyces pombe Postgenome Database (SPD) contains the results of systematic analysis of protein expression and localization based on fission yeast ORFeome cloning as well as basic information on genes and proteins.
The ORF collection currently covers 99.2% of the fission yeast protein-coding genes and pseudogenes that are reported by the Wellcome Trust Sanger Institute.

1. Schizosaccharomyces pombe ORFeome clones

1.1 Strain information

Species

Schizosaccharomyces pombe

Strain

wild-type strain JY3

1.2 Clone information

Entry clones (SpENT)
Vector	pDONR201 (Kan^r), if not specified individually
Insert	PCR amplification of GENOMIC DNA Amplified with 5'-G GGG ACA AGT TTG TAC AAA AAA GCA GGC TCT CAT ATG-(template specific sequence)-3' and 5'-GGG GAC CAC TTT GTA CAA GAA AGC TGG GTA-(template specific sequence)-3' as primers.
Host	DH5alpha, E. coli

FLAG2-His6-tagged ORFs (SpFFH)
Vector	pDUAL-FFH1c (Amp^r), if not specified individually
Insert	Corresponding gene was cloned from the entry clone to pDUAL-FFH1c (RDB 6142) using the Gateway technology.
Host	DH5alpha, E. coli

YFP-FLAG-His6-tagged ORFs (SpYFH)
Vector	pDUAL-YFH1c (Amp^r), if not specified individually
Insert	Corresponding gene was cloned from the entry clone to pDUAL-YFH1c (RDB 6151) using the Gateway technology.
Host	DH5alpha, E. coli

1.3 Reference

Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Horaoka Y., Horinouchi S., Yoshida M. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nature Biotech., 24, 841-847.

A dataset including the cloning status of the ORFeome, protein expression levels, and localizome data is available by downloading the following CSV file:
ORFeome_Localizome_data.csv (2008.03.11, 1.18 mb)
(This file is also available at S. pombe postgenome database, http://www.riken.jp/SPD/index.html)

A map of empty well in a 96-well plate of the entry clone set:
RDB6116.pdf (2009.08.28)

2. Distribution of clones

2.1 Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER must cite the following literature(s). (Matsuyama et al., 2006, Nature Biotech., 24, 841-847.).
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER must state an acknowledgement to the DEPOSITOR.
When the USER wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the USER must obtain a prior written permission from the DEPOSITOR .
When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the USER's name and his/her affiliation.

2.2 How to obtain clones?

Single clone

RDB 5820 (SpENT) Entry clone
RDB 6106 (SpFFH) FLAG2-His6-tagged ORF
RDB 6107 (SpYFH) YFP-FLAG-His6-tagged ORF
Clone(s) is shipped as DNA solution in TE buffer in individual tube.
Please specify ID of individual clone(s).

In the case you order two or more clones, please write in FormA, for example,
    01/A01 SPBC1711.15c (SpENT)
    01/A01 SPBC1711.15c (SpYFH)
    01/B11 SPAC16A10.05c (SpYFH)
    01/E01 SPCC191.04c (SpFFH)

Clone set

RDB 6116 entry clone set
RDB 6117 FLAG2-His6-tagged ORF ser
RDB 6118 YFH-tagged ORF set
Clones are shipped as frozen recombinant E. coli in 96-well plate with dryice.

Vector series

pDUAL vector series
pDUAL2 vector series
pREP vector series
Please also visit here.
Clone(s) is shipped as DNA solution in TE buffer in individual tube.
Please specify ID of individual clone(s).

Forms

Material Transfer Agreement for S. pombe ORFeome Clones FormC6107np
(We ask a signature of the Athorized Representative by an administrator of intellectual property.)
Order Form (FormA)
SCHEDULE A, if your clone contains GFP, CFP and YFP. (GFP bioresources)

Procedure
Fee

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2011.02.26