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Gene Analysis - Microbe

S. pombe ORFeome Clones

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Schizosaccharomyces pombe ORFeome clones were deposited by Dr. Minoru Yoshida, Chemical Genetics Lagoratory, RIKEN; These consists of 4,900 entry clones. Individual clones are available.

Press release
Localization data is available at
S. pombe gene information database
http://www.riken.jp/SPD/index.html

The Schizosaccharomyces pombe Postgenome Database (SPD) contains the results of systematic analysis of protein expression and localization based on fission yeast ORFeome cloning as well as basic information on genes and proteins.
The ORF collection currently covers 99.2% of the fission yeast protein-coding genes and pseudogenes that are reported by the Wellcome Trust Sanger Institute.


1. . Distribution of clones
1.1 Terms and conditions for distribution
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER must cite the following literature(s). (Matsuyama et al., 2006, Nature Biotech., 24, 841-847.).
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER must state an acknowledgement to the DEPOSITOR.
When the USER wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the USER must obtain a prior written permission from the DEPOSITOR .
When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the USER's name and his/her affiliation.
1.2 How to obtain clones?

Individual Clones

Clone(s) is shipped as DNA solution in TE buffer in an individual tube.
Please specify ID of individual clone(s).

Obtain data of individual clone.
Plate Row Column 
- -
 
Find S. pombe Clones
in the A to Z List.
Go to
 

There are three kinds of clones as follows.
Entry clone (SpENT), Cat#. RDB 5820
FLAG2-His6-tagged ORF (SpFFH), Cat#. RDB 6106
YFP-FLAG-His6-tagged ORF (SpYFH), Cat#. RDB 6107
Please reffer data sheet for datail.
    Please write clone ID in FormA, for example,
        01/A01 SPBC1711.15c (SpENT)
        01/A01 SPBC1711.15c (SpYFH)
        01/B11 SPAC16A10.05c (SpYFH)
        01/E01 SPCC191.04c (SpFFH)

Clone set are shipped as frozen recombinant E. coli in 96-well plate with dryice.

Order Forms

  • Order Form (FormA, download site)
    Please complete the form with your shipping information including your account number of an international courier, for example FedEx, World Courier, TNT Express, DHL Global Forwarding and others.
  • Material Transfer Agreement for S. pombe ORFeome Clones FormC6107np, PDF
    We would like to ask a signature of the Authorized Representative of a recipient institution.
    In the the Section 2(a), please write your purpose of use of clone in 10 to 20 words.
  • SCHEDULE A, if your clone contains GFP, CFP and YFP. (GFP bioresources)

Please visit this page for further information of distribution and fees.

Send forms by regular mail or scanned PDF by e-mail:
    The DNA Bank, RIKEN BioResource Center,
    3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
    E-mail: dnabank@brc.riken.jp Fax: (+81)-29-836-9120


Clone set

Clone set is shipped as frozen glycerol stock in 96-well plate with dryice.
Please contact us for further information of distribution and fees.
    The DNA Bank, RIKEN BioResource Center,
    3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
    E-mail: dnabank@brc.riken.jp Fax: (+81)-29-836-9120

Empty vectors

pDUAL vector series
pDUAL2 vector series
pREP vector series
Please find vectors here.
Clone(s) is shipped as DNA solution in TE buffer in an individual tube.
Please specify ID of individual clone(s).

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2012.10.22