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Gene Analysis - Microbe

Thermus thermophilus Disruption plasmid

  Disruption (ja)   Disruption (en)   Data Sheet   List of Genes   Expression Plasmid

Thermus thermophilus HB8 Disruption plasmids were constructed by "Whole-Cell Project of a Model Organism, Thermus thermophilus HB8" (http://www.thermus.org/) and deposited by Dr. Seiki Kuramitsu, SR System Biology Research Group, RIKEN Harima Institute; Individual clones are available.

The gene disruptant of T. thermophils HB8 can be easily prepared by adding this plasmid into the culture medium. The target gene in this plasmid was replaced by the thermostable kanamycine resistant gene from Staphylococcus aureus. The length of the homologous region outside of the target gene is about 500 bp (only 10 bp of the target geneare left in both sides) .

How to obtain clones?

Disruption Clone


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Outside database



Forms


Sending forms
Plasmid clones for disrupting Thermus thermophilus HB8 gene.
Clone(s) is shipped as DNA solution in TE buffer in individual tube.
Please amplify this plasmid clone using E. coli host strain (ex. Stbl2, Invitrogen) suitable for the cloning of unstable inserts such as direct repeats.
Please find ID of individual clone(s) using the following outside database
http://www.srg.harima.riken.jp/h_db/index.html
or this summary table.
Please specify ID (ex. TDs01A01) of individual clone(s).

Please indicate Terms and Conditions for Distribution in MTA as follows:
  • In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER is expected to cite the following literature(s). (Yokoyama et al., 2000, Nature Struct. Biol. 7, 943-945).

Please complete forms:
    Order Form, FormA (MS-Word)
    Material Transfer Agreement, FormC (pdf)
Please visit this page for further information of distribution.

Send forms:
    The DNA Bank, RIKEN BioResource Center,
    3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
    Fax: (+81)-29-836-9120
    E-mail: dnabank@brc.riken.jp


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2012.10.22