BioResource Center RIKEN BRC DNA BANK

Resources (Japanese / English)  > Shuttle vector (Japanese / English)  > Shuttle Vector of Sugano Full-Length Human cDNA

List of adenovirus shuttle vector of Sugano full-length human cDNA

These shuttle vectors were constructed for generation of recombinant adenovirus based on pAxCALNLw cosmid vector. Full-length human cDNA produced by 'oligo-cap method' was inserted into SwaI site of pAxCALNLw.
Recombinant adenovirus can be obtained by 'COS-TPC method'.



go to alphabetical list

Some clones can be truncated by digestion with SalI or NruI restriction enzyme followed by self-ligation.

Example of truncation of shuttle vector

Plasmid clone pIRES-EYFP was digested with SmaI and XhoI, treated with Klenow enzyme to obtain blunt end termini, and the fragment of EYFP was ligated into SwaI site of a cosmid vector pAxCALNLw to obtain pAxCALNLEYFP. The shuttle vector pAxCALNLEYFP was then digested with SalI and ligated by itself to obtain a plasmid vector pxCALNLEYFP.
pxCALNLEYFP was then transfected to COS1 cells with or without pxCANCre (RDB 1675), which expresses Cre recombinase.




Expression of YFP was detected in cells transfected pxCALNLEYFP with pxCANCre (right) but not pCALNLEYFP alone (left).


go to Head

CategoryLocusRefSeqSugano
clone no.
RDB no.
3' processingCSTF2/CstF64NM_001325CBR020763549
CSTF2T/CstF64-relatedNM_015235CBR020763549
apoptosisApo-2L/TNFSF10/TRAILNM_003810DMC065753603
Apo-2L/TNFSF10/TRAILNM_003810HRC027204677
Apo-2L/TNFSF10/TRAILNM_003810HRC041664682
ARHGDIANM_004309CAS011223494
ARHGDIANM_004309HRC084234693
BADNM_004322adSU020723488
BAG1NM_004323DMC083123606
BIRC4/XIAPNM_001167COL072803578
BIRC5/API4NM_001168CAS117443521
c-junNM_002228HRT069424711
CASP1/Caspase 1NM_001223CAS035283498
CASP1/Caspase 1NM_001223CBL016553526
CASP1/Caspase 1NM_001223HSI009654716
CASP7NM_001227HEP056344639
CASP8/FLICENM_001228HEP145564661
CASP9/APAF-3NM_001229adSH013483483
CD40/TNFRSF5NM_001250HRC009134672
CD40/TNFRSF5NM_001250HRC026834676
CD40/TNFRSF5NM_001250HRC053954687
CDK2NM_001798adKA023563472
CDK2NM_001798CAE073293492
CDKN1A/Cip1/p21NM_000389COL062643576
CDKN1B/Kip1/p27NM_004064HEP085834645
CSTA/Cystatin ANM_005213DMC065733602
DFFA/DFF45/ICADNM_004401HRC072624690
DFFA/DFF45/ICADNM_004401COL095793581
DFFB/CAD/DFF40NM_004402CBR015963545
FADDNM_003824DMC091283610
FADDNM_003824HRC112474703
GADD45BNM_015675DMC003103584
GAS2NM_005256DMC001413583
GAS2NM_005256HEP178894666
IF2S1/eIF2alphaNM_004094adSU007183486
LMNA/Lamin ANM_005572adKA017283467
LMNA/Lamin ANM_005572adKA033093476
LMNA/Lamin ANM_005572adSE007053479
LMNA/Lamin ANM_005572HRC079784692
MYD88NM_002468HEP146494662
PCNANM_002592DMC048553597
PCNANM_002592DMC062533600
PCNANM_002592HSI014494721
PRF1/Perforin 1NM_005041DMC065163601
RELA/p65NM_021975HEP014454631
RELA/p65NM_021975HEP129314657
RIPK2/RIP2/RICKNM_003821HEP209884669
SHC1NM_003029HEP090144648
TLR7NM_016562HSI008604715
TNFRSF10A/DR4NM_003844HEP133374658
TNFRSF10A/DR4/TRAIL-R1NM_003844CAS097553516
TNFRSF10C/TRAIL R3/TRIDNM_003841HRC031294678
TNFRSF10D/DcR2/TRAILR4/TRUNDDNM_003840HRC031294678
TNFSF10/TRAILNM_003810HRC036604680
TRAF2NM_021138HSI013054719
TRAF4NM_004295DMC019513588
CD/extracellularCD40/TNFRSF5NM_001250HRC009134672
CD40/TNFRSF5NM_001250HRC026834676
CD40/TNFRSF5NM_001250HRC053954687
CD44NM_000610HRC023394674
CD44NM_000610HRC046904684
CD74NM_004355adKA017223466
CD74NM_004355adKA022713470
CD74NM_004355adKA022723471
CD74NM_004355adKA031913474
CD74NM_004355adSE014023481
GJB1/Connexin-32NM_000166HSI009864717
cell activationBADNM_004322adSU020723488
c-junNM_002228HRT069424711
CD40/TNFRSF5NM_001250HRC009134672
CD40/TNFRSF5NM_001250HRC026834676
CD40/TNFRSF5NM_001250HRC053954687
CD86/B7-2NM_006889CBL013323525
CD86/B7-2NM_006889CBL062803537
CHRNB1/ACHRBNM_000747HEP068134643
IFNGR1/IFN-GRalphaNM_000416HEP001153611
IFNGR1/IFN-GRalphaNM_000416HEP028244634
IFNGR1/IFN-GRalphaNM_000416HEP028744635
IL18NM_001562DMC045213594
IL18NM_001562HSI010334718
cell adhesionc-junNM_002228HRT069424711
CAPN5/Calpain 5NM_004055CAS018833495
PFN1/profilin1NM_005022HSI014224720
PFN2/profilin2NM_002628CBR034203550
PFN2/profilin2NM_002628DMC042103593
SHC1NM_003029HEP090144648
TACSTD1/Ep-CAMNM_002354HSI017834977
VCL/vinculinNM_003373adSH013263482
cell cyclec-junNM_002228HRT069424711
CCNA1/Cyc A1NM_003914CBL057613534
CCNA1/Cyc A1NM_003914CBR049343557
CCNA2/Cyc A2NM_001237DMC045473595
CCNB2/Cyc B2NM_004701CAS097513515
CCNH/Cyc HNM_001239CAS090723510
CCNH/Cyc HNM_001239DMC020653589
CDC25ANM_001789HEP119374653
CDK2NM_001798adKA023563472
CDK2NM_001798CAE073293492
CDK7/CAKNM_001799CAS080833503
CDK7/CAKNM_001799CBL094243539
CDKN1A/Cip1/p21NM_000389COL062643576
CDKN1B/Kip1/p27NM_004064HEP085834645
CNN1/Calponin1NM_001299CAS082143504
CNN2/Calponin2NM_004368HRC060344689
CNN2/Calponin2NM_004368HRC092154697
INK4Bp15/CDKN2BNM_004936adSE011013480
INK4Bp15/CDKN2BNM_004936COL084443579
INK4p16/CDKN2ANM_000077CAS062483502
NBS1/NIBRINNM_002485COL091893580
RAD51L1NM_002877CBL051433530
SMT3H2/Smt3BNM_006937CAS083553506
SMT3H2/Smt3BNM_006937CAS124843522
SMT3H2/Smt3BNM_006937DMC040513591
TRAF2NM_021138HSI013054719
cell cycle/proliferationPCNANM_002592DMC048553597
PCNANM_002592DMC062533600
PCNANM_002592HSI014494721
chemokineSCYD1/FractalkineNM_002996CBR076533566
circadian clockCSNK1E/CK-IepsilonNM_001894CAS047703500
CSNK1E/CK-IepsilonNM_001894HRC039814681
DbpNM_001352adKA022533469
DbpNM_001352HRC026634675
cytokinec-junNM_002228HRT069424711
CASP1/Caspase 1NM_001223CAS035283498
CASP1/Caspase 1NM_001223CBL016553526
CASP1/Caspase 1NM_001223HSI009654716
CD58/LFA3NM_001779HEP019794632
CSF3/G-CSFNM_000759CAS033203497
CSF3/G-CSFNM_000759DMC070253605
CXCR4/FusinNM_003467DMC005763585
EFNA1/Ephrin-A1NM_004428HRC005914670
EFNA1/Ephrin-A1NM_004428HRC093964698
EFNA1/Ephrin-A1NM_004428HRC132234706
EGFRNM_005228HRC115194704
FGF1/acidic FGFNM_000800CBL012913524
HMGIYNM_002131HRC089584695
HMGIYNM_002131HRC097564700
IFNAR1NM_000629HEP003774626
IFNAR1NM_000629HEP046024637
IFNGR1/IFN-GRalphaNM_000416HEP001153611
IFNGR1/IFN-GRalphaNM_000416HEP028244634
IFNGR1/IFN-GRalphaNM_000416HEP028744635
IL18NM_001562DMC045213594
IL18NM_001562HSI010334718
IL1B/IL-1 betaNM_000576CAS083663507
IL1B/IL-1 betaNM_000576CAS083913508
IL1B/IL-1 betaNM_000576CAS093233513
IL1B/IL-1 betaNM_000576CAS098423518
IL3RA/IL-3R alphaNM_002183CBL057313533
IL3RA/IL-3R alphaNM_002183CBR095263572
IL3RA/IL-3R alphaNM_002183DMC068583604
IL6NM_000600CAS043623499
IL6ST/gp130NM_002184CAS091363512
IL6ST/gp130NM_002184DMC037703590
IL7NM_000880COL033333574
IL8NM_000584CAS028843496
IL8NM_000584CAS050873501
IL8NM_000584CAS082663505
IL8NM_000584DMC046443596
LTBR/Lymphotoxin beta receptorNM_002342adSU012293487
MADH2/SMAD2NM_005901CAS098763519
MYD88NM_002468HEP146494662
NTRK2/Trk BNM_006180CBR018463546
SHC1NM_003029HEP090144648
STAT3NM_003150HRC096514699
TNFRSF11B/OsteoprotegerinNM_002546COL099573582
CytoskeltalAP1G1/gamma-AdaptinNM_001128HEP057424641
MAP1LC3A/MAP1, light chainNM_032514CBR034943551
MAP2NM_002374CBR007863542
VCL/VinculinNM_003373adSH013263482
VIM/VimentinNM_003380adSH015833484
developmentCAMK1GNM_020439CBR090033571
CAMK2ANM_015981CBR057923560
CAMK2ANM_015981CBR070453564
CAMK2BNM_001220CBR050933558
CAMK2BNM_001220CBR068603563
CAMK2BNM_001220CBR085263568
MYC/c-MycNM_002467HEP134584659
MYD88NM_002468HEP146494662
DNA synthesisBin1/Amphiphysin IINM_004305HRC102434701
Bin1/Amphiphysin IINM_004305HRC105944702
PCNANM_002592DMC048553597
PCNANM_002592DMC062533600
PCNANM_002592HSI014494721
G proteinARF1/ADP ribosylation factor 1NM_001658CBR020303548
ARF1/ADP ribosylation factor 1NM_001658DMC017333587
ARF1/ADP ribosylation factor 1NM_001658HRC085444694
hematopoeisisCSF3/G-CSFNM_000759CAS033203497
CSF3/G-CSFNM_000759DMC070253605
CXCR4/FusinNM_003467DMC005763585
IFNGR1/IFN-GRalphaNM_000416HEP001153611
IFNGR1/IFN-GRalphaNM_000416HEP028244634
IFNGR1/IFN-GRalphaNM_000416HEP028744635
IL18NM_001562DMC045213594
IL18NM_001562HSI010334718
IL6NM_000600CAS043623499
IL8NM_000584CAS028843496
IL8NM_000584CAS050873501
IL8NM_000584CAS082663505
IL8NM_000584DMC046443596
immunologyBST2NM_004335adKA020333468
c-junNM_002228HRT069424711
C1q alphaNM_015991DMC088133607
Calcineurin A alphaNM_000944CBR055673559
CD18/ITGB2NM_000211adKA034363477
CD32/FCGR2ANM_021642HRT018644707
CD40/TNFRSF5NM_001250HRC009134672
CD40/TNFRSF5NM_001250HRC026834676
CD40/TNFRSF5NM_001250HRC053954687
CD44NM_000610HRC023394674
CD44NM_000610HRC046904684
CD86/B7-2NM_006889CBL013323525
CD86/B7-2NM_006889CBL062803537
CXCR4/FusinNM_003467DMC005763585
IL18NM_001562DMC045213594
IL18NM_001562HSI010334718
IL1B/IL-1 betaNM_000576CAS083663507
IL1B/IL-1 betaNM_000576CAS083913508
IL1B/IL-1 betaNM_000576CAS093233513
IL1B/IL-1 betaNM_000576CAS098423518
JUNBNM_002229adKA032723475
JUNBNM_002229adSH019063485
JUNBNM_002229COL062793577
MASP1NM_001879HRT027924710
MASP1NM_001879HRT072244712
MYC/c-MycNM_002467HEP134584659
PRF1/Perforin 1NM_005041DMC065163601
PRKACBNM_002731CBL007033523
PRKAR1ANM_002734CBR087213569
PRKAR1ANM_002734HEP124294656
PSEN1/presenilin1NM_000021CBL077523538
RAC1NM_006908adSE004233478
RAC1NM_006908CBR002603540
SELPLG/P-Selectin LNM_003006adSU022543489
SHC1NM_003029HEP090144648
TACINM_012452HSI001674713
THY1NM_006288CAS099593520
TRAF2NM_021138HSI013054719
VIPNM_003381HSI007664714
MatrixMMP11/Matrix metalloproteinase-11NM_005940DMC040533592
MMP11/Matrix metalloproteinase-11NM_005940DMC089573608
MMP11/Matrix metalloproteinase-11NM_005940HEP005634629
MMP13/Matrix metalloproteinase-13NM_002427DMC059083598
TIMP1NM_003254CAS087353509
TIMP1NM_003254CAS097093514
TIMP1NM_003254CAS097693517
TIMP4NM_003256adKA027363473
neuroscienceARRB1/arrestinB1NM_004041CAE102223493
ARRB1/arrestinB1NM_004041HRC079234691
CCK/CholecystokininNM_000729CBR015953544
CCK/CholecystokininNM_000729CBR038623552
CCK/CholecystokininNM_000729CBR066523562
CCK/CholecystokininNM_000729CBR074653565
CHGA/Chromogranin ANM_001275CBR015093543
EDN1/Endothelin-1NM_001955DMC059623599
GFAPNM_002055CBL024393528
GFAPNM_002055CBL031553529
GFAPNM_002055CBL053763532
GFAPNM_002055CBL060823536
GFAPNM_002055CBR019673547
PSEN1/presenilin1NM_000021CBL077523538
PSEN2/presenilin2NM_000447CAE058293491
SNAP25NM_003081CBL052463531
SNAP25NM_003081CBR041703553
SNAP25NM_003081CBR043283554
SNAP25NM_003081CBR049273556
SNCANM_000345CBR058423561
SNCANM_000345CBR096923573
VIP/Vasoactive intestinal paptideNM_003381HSI007664714
NO metabolismSOD1/Superoxide dismutase 1NM_000454CAS090733511
phosphorylationCAMK2A/CaM Kinase II alphaNM_015981CBR057923560
CAMK2A/CaM Kinase II alphaNM_015981CBR070453564
CAMK2A/CaM Kinase II alphaNM_015981CBR085263568
DAPK3/Zip kinaseNM_001348CBL017643527
DAPK3/Zip kinaseNM_001348CBR080663567
MAPK7/ERK5NM_002749COL057203575
PRKCG/PKCgammaNM_002739CBR007743541
PRKCG/PKCgammaNM_002739CBR046653555
PRKCG/PKCgammaNM_002739CBR087333570
ReceptorCXADR/CARNM_001338HEP004944627
CXADR/CARNM_001338HEP137584660
Transcription factorc-JunNM_002228HRT069424711
FOSB/Fos-BNM_006732adSU023633490
FOSL2/Fra2NM_005253HRC006854671
HDAC2NM_001527DMC091193609
JUNBNM_002229adKA032723475
JUNBNM_002229adSH019063485
JUNBNM_002229COL062793577
RELA/p65NM_021975HEP014454631
RELA/p65NM_021975HEP129314657
STAT3NM_003150HRC096514699
TFDP2/DP-2NM_006286CBL059133535
TFDP2/DP-2NM_006286DMC014263586
go to Head



2005.10.07