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List of adenovirus shuttle vector of Sugano full-length human cDNA

These shuttle vectors were constructed for generation of recombinant adenovirus based on pAxCALNLw cosmid vector. Full-length human cDNA produced by 'oligo-cap method' was inserted into SwaI site of pAxCALNLw.
Recombinant adenovirus can be obtained by 'COS-TPC method'.

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Example of truncation of shuttle vector

Plasmid clone pIRES-EYFP was digested with SmaI and XhoI, treated with Klenow enzyme to obtain blunt end termini, and the fragment of EYFP was ligated into SwaI site of a cosmid vector pAxCALNLw to obtain pAxCALNLEYFP. The shuttle vector pAxCALNLEYFP was then digested with SalI and ligated by itself to obtain a plasmid vector pxCALNLEYFP.
pxCALNLEYFP was then transfected to COS1 cells with or without pxCANCre (RDB 1675), which expresses Cre recombinase.

Expression of YFP was detected in cells transfected pxCALNLEYFP with pxCANCre (right) but not pCALNLEYFP alone (left).

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2005.10.07