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Control Vectors

Luciferase

Name of cloneTypeFormulaCat no.
pCMVluc+
 Expression vector of firefly luciferase
fireflyDNA solutionRDB03988
pGL3-RSV
 Expression vector of firefly luciferase, RSV promoter
fireflyDNA solutionRDB06411
pCAluc+
 Expression vector of firefly luciferase
fireflyDNA solutionRDB03989
pCAcc-Luc+
 Expressing luciferase under the control of CAG promoter
fireflyDNA solutionRDB02443
pLRICALuc+
 Shuttle vector to generate recombinant adenovirus
fireflyDNA solutionRDB02444
pTcf7wtluc
 Reporter plasmid carring TCF-binding consensus sequence
fireflyDNA solutionRDB02968
pTcf7mtluc
 Reporter plasmid carring TCF-binding consensus sequence
fireflyDNA solutionRDB02969
ptk-luc
 Reporter construct with luciferase expression under tk promoter
fireflyDNA solutionRDB07031
pFGF4tk-luc
 Reporter construct with luciferase expression under FGF4 and tk promoter
fireflyDNA solutionRDB07032
AxCA Luc+
 Recombinant adenovirus expressing luciferase gene
fireflyDNA solutionRDB02453
pKM2L
 Reporter construct with Renilla luciferase gene
RenillaDNA solutionRDB04026
pKM2L-pvTK
 HSV herpes simplex virus thymidine kinase (HSV-TK)
RenillaDNA solutionRDB05549
pKM2L-pvSV40
 SV40 simian virus 40 early enhancer/promoter region (SV40)
RenillaDNA solutionRDB05550
pKM2L-pvCMV
 CMV cytomegalovirus immediate enhancer/promoter (CMV)
RenillaDNA solutionRDB05551
pRL-CMV mut1
 Expression vector of Renilla luciferase, mutated CMV promoter
RenillaDNA solutionRDB06408
pRL-CMV mut2
 Expression vector of Renilla luciferase, mutated CMV promoter
RenillaDNA solutionRDB06409
pRL-RSV
 Expression vector of Renilla luciferase, RSV promoter
RenillaDNA solutionRDB06410

LacZ

RDBName of cloneRunning title
RDB06072pCMFlag_lacZExpression vector of lacZ
RDB07081pCMFlag_lacZExpression vector of lacZ
RDB01676pxCANLacZA plasmid expressing a beta-galactosidase tagged with a NLS
RDB01870pCAZ 2E. coli LacZ genomic DNA
RDB02037pLCAZ2LacZ clone
RDB01680pCALNLZA control plasmid to introduce LacZ gene into mammalian chromosome
RDB03380pAxCAiLacZitShuttle vector to generate rAd harbouring E. coli LacZ
RDB02104pAxCAZ3Shuttle vector to generate rAd expressing E. coli lacZ
RDB01589pAxCEAprZShuttle vector to generate rAd expressing E. coli lacZ gene driven by CEA promoter
RDB07061pAxEFFlag_LacZit2Shuttle vector to generate recombinant adenovirus
RDB01699pRx nZ ires NeoNls (nuclear localization signal), lacZ, ires, neo
RDB02976pMFGlacZShuttle vector to generate recombinant retrovirus cexpressing E. coli lacZ
RDB02039pAxPSZShuttle vector to generate rAd expressing E. coli lacZ
RDB01590pAxRxZ"Shuttle vector to generate rAd expressing E. coli lacZ gene driven by Rx promoter
RDB01503pAACAZAAV vector expressing E. coli lacZ
RDB02038AxPSZRecombinant adenovirus expressing E. coli lacZ regulated by human PSA promoter
RDB02726AxCALacZRecombinant adenovirus expressing beta-galactosidase under the control of CAG promoter
RDB01750AxCALNLNZRecombinant adenovirus expressing E. coli lacZ
RDB03211AxCANLacZRecombinant adenovirus expressing E. coli lacZ
RDB02036AxCAZ2-F/K20Recombinant adenovirus expressing lacZ regulated by CAG promoter. Fiber-mutant, F/K20
RDB02196AxCAZ3Recombinant adenovirus expressing E. coli lacZ regulated by CAG promoter
RDB02172AxCAZ3 F/asK21MSH betaRecombinant adenovirus expressing E. coli lacZ. Fiber-mutant, F/asK21MSH beta
RDB02175AxCAZ3 F/gsK21MSH betaRecombinant adenovirus expressing E. coli lacZ. Fiber-mutant, F/gsK21MSH beta
RDB02170AxCAZ3 F/gsMSH betaRecombinant adenovirus expressing E. coli lacZ. Fiber-mutant, F/gsMSH beta
RDB02258AxCAZ3-F/540SRecombinant adenovirus expressing E. coli lacZ
RDB02240AxCAZ3-F/asATRecombinant adenovirus expressing E. coli lacZ
RDB02230AxCAZ3-F/asGRPRecombinant adenovirus expressing E. coli lacZ
RDB02231AxCAZ3-F/asGRP (4th)Recombinant adenovirus expressing E. coli lacZ
RDB04375AxCAZ3-F/asGRP27Recombinant adenovirus expressing E. coli lacZ
RDB04376AxCAZ3-F/asGRP27NMRecombinant adenovirus expressing E. coli lacZ
RDB02165AxCAZ3-F/asMSH alphaRecombinant adenovirus expressing lacZ. Fiber-mutant, F/asMSH alpha mutant
RDB02236AxCAZ3-F/asSecRecombinant adenovirus expressing E. coli lacZ
RDB02237AxCAZ3-F/asSec (4th)Recombinant adenovirus expressing E. coli lacZ
RDB02194AxCAZ3-F/RGDRecombinant adenovirus expressing E. coli lacZ. Fiber-mutant, F/RGD
RDB03937AxCAZ3-F40STAARecombinant adenovirus expressing E.coli lacZ
RDB03954Ax3CAZ3-F405hiRGDRecombinant adenovirus expressing E. coli lacZ
RDB03931Ax3CAZ3-F40SthiRGDRecombinant adenovirus expressing E. coli lacZ

Cre-loxP and FLP-FRT System

Plasmid clone pxCALNLEYFP (RDB06232) can be used for a positive control of Cre-mediated gene regulation. The pxCALNLEYFP contains CAG promoter, loxP-Neo-loxP stuffer sequence and coding region of EYFP. In this example, the pxCALNLEYFP was transfected to COS1 cells with or without pxCANCre (RDB01675), which expresses Cre recombinase.




Expression of YFP was detected in cells transfected pxCALNLEYFP with pxCANCre (right) but not pCALNLEYFP alone (left).

RDBName of cloneRunning titleType
RDB08123AxEFhFLPeRecombinant adenovirus expressing humanized FLPeRecombinant adenovirus
RDB08124pAxEFhFLPeit2Shuttle vector to generate recombinant adenovirus expressing humanized FLPeShuttleVector
RDB07083pxCAhFLPeExpression vector of site-specific recombinase, FLPDNA clone
RDB01748AxCANCreRecombinant adenovirus expressing recombinase Cre with nuclear localization signalRecombinant Adenovirus
RDB06073pKM2CReporter construct with Cre recombinase geneVector
RDB01675pxCANCreA plasmid expressing the recombinase Cre tagged with a nlsDNA clone
RDB08998pCAGGS-CreExpression clone of Cre recombinase.DNA clone
RDB09596pPyCAG-Cre-IPExpression clone of Cre recombinase. Puromycin resistance.DNA clone
RDB12117pPyCAG-CreGR-IBExpression clone of Cre-GR LBD fusion regulatable by dexamethasone. BSD resistance.DNA clone
RDB07106pPyCAG-CreGR-IDExpression clone of Cre-GR LBD fusion regulatable by dexamethasone. HisD resistance.DNA clone
RDB03347AxCALNLEGFP (forward)Recombinant adenovirus harboring enhanced green fluorescent protein cDNARecombinant adenovirus
RDB03251pAxCALNLBFP (forward)Shuttle vector for rAd harboring blue fluorescent protein cDNAShuttleVector
RDB03252pAxCALNLBFP (reverse)Shuttle vector for rAd harboring blue fluorescent protein cDNAShuttleVector
RDB03259pAxCALNLEGFP (forward)Shuttle vector for rAd harboring enhanced green fluorescent protein cDNAShuttleVector
RDB03260pAxCALNLEGFP (reverse)Shuttle vector for rAd harboring enhanced green fluorescent protein cDNAShuttleVector
RDB03261pAxCALNLEYFP (forward)Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNAShuttleVector
RDB03262pAxCALNLEYFP (reverse)Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNAShuttleVector
RDB06232pxCALNLEYFPExpression vector of EYFPDNA clone
RDB01750AxCALNLNZRecombinant adenovirus expressing E. coli lacZRecombinant Adenovirus
RDB01862pCALNL5A cassette to introduce cDNA into mammalian chromosomeVector
RDB01674pCALNLwA cassette to introduce cDNA into mammalian chromosomeVector
RDB01680pCALNLZA control plasmid to introduce LacZ gene into mammalian chromosomeDNA clone
RDB01679pCALwLA cassette plasmid to insert cDNA into downstream CAG promoterVector
RDB03448pUAd5LCALNL(W1975C1F)Shuttle vector to generate recombinant adenovirusVector
RDB03446pUAd5LCALNL(W1975C23R)Shuttle vector to generate recombinant adenovirusVector
RDB01677pULwLA cassette plasmid to insert stuffer DNA between two loxP siteVector
RDB04397pUSwSA cassette plasmid for inserting DNA between two loxP S sequences in the same orientationVector
RDB04396pUVwVA cassette plasmid for inserting DNA between two loxP V sequences in the same orientationVector

(T.M. 2004.08.22)

2014.09.13