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Clontech Laboratories, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

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Control Vectors


Cre-loxP and FLP-FRT System

Plasmid clone pxCALNLEYFP (RDB06232) can be used for a positive control of Cre-mediated gene regulation. The pxCALNLEYFP contains CAG promoter, loxP-Neo-loxP stuffer sequence and coding region of EYFP. In this example, the pxCALNLEYFP was transfected to COS1 cells with or without pxCANCre (RDB01675), which expresses Cre recombinase.

Expression of YFP was detected in cells transfected pxCALNLEYFP with pxCANCre (right) but not pCALNLEYFP alone (left).

Catalog no. Name of resource
RDB08123 AxEFhFLPe
Recombinant adenovirus expressing humanized FLPe
RDB08124 pAxEFhFLPeit2
Shuttle vector to generate recombinant adenovirus expressing humanized FLPe
RDB07083 pxCAhFLPe
Expression vector of site-specific recombinase, FLP
RDB01748 AxCANCre
Recombinant adenovirus expressing recombinase Cre with nuclear localization signal
RDB06073 pKM2C
Reporter construct with Cre recombinase gene
RDB01675 pxCANCre
A plasmid expressing the recombinase Cre tagged with a nls
RDB08998 pCAGGS-Cre
Expression clone of Cre recombinase.
RDB09596 pPyCAG-Cre-IP
Expression clone of Cre recombinase. Puromycin resistance.
RDB12117 pPyCAG-CreGR-IB
Expression clone of Cre-GR LBD fusion regulatable by dexamethasone. BSD resistance.
RDB07106 pPyCAG-CreGR-ID
Expression clone of Cre-GR LBD fusion regulatable by dexamethasone. HisD resistance.
RDB03347 AxCALNLEGFP (forward)
Recombinant adenovirus harboring enhanced green fluorescent protein cDNA
RDB03251 pAxCALNLBFP (forward)
Shuttle vector for rAd harboring blue fluorescent protein cDNA
RDB03252 pAxCALNLBFP (reverse)
Shuttle vector for rAd harboring blue fluorescent protein cDNA
RDB03259 pAxCALNLEGFP (forward)
Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA
RDB03260 pAxCALNLEGFP (reverse)
Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA
RDB03261 pAxCALNLEYFP (forward)
Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNA
RDB03262 pAxCALNLEYFP (reverse)
Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNA
RDB06232 pxCALNLEYFP
Expression vector of EYFP
RDB01750 AxCALNLNZ
Recombinant adenovirus expressing E. coli lacZ
RDB01862 pCALNL5
A cassette to introduce cDNA into mammalian chromosome
RDB01674 pCALNLw
A cassette to introduce cDNA into mammalian chromosome
RDB01680 pCALNLZ
A control plasmid to introduce LacZ gene into mammalian chromosome
RDB01679 pCALwL
A cassette plasmid to insert cDNA into downstream CAG promoter
RDB03448 pUAd5LCALNL(W1975C1F)
Shuttle vector to generate recombinant adenovirus
RDB03446 pUAd5LCALNL(W1975C23R)
Shuttle vector to generate recombinant adenovirus
RDB01677 pULwL
A cassette plasmid to insert stuffer DNA between two loxP site
RDB04397 pUSwS
A cassette plasmid for inserting DNA between two loxP S sequences in the same orientation
RDB04396 pUVwV
A cassette plasmid for inserting DNA between two loxP V sequences in the same orientation
RDB14533 pCS2-OlCre
Expression vector of P1 phage Cre recombinase, codon-optimized for Oryzias latipes (japanese medaka)

 

Tet Inducible System

Catalog no. Name of resource
RDB13211 pAAV-Enk-FtTA
AAV vector of tTA under the control of Enkephalin promoter
RDB13212 pAAV-SP-FtTA
AAV vector of tTA under the control of Substance P promoter
RDB01683 pTet NtTA
Tet-transactivator driven by tet-operator promoter
RDB01684 pCM-NtTA
Tet-transactivator driven by CM promoter
RDB09232 pBlKS hCMV*-1 b-globinpA
Expression vector of tet-inducible CMV promoter b-globin poly(A) casette.
RDB12109 pBRPyCAG-tTAFF-IN
Expression vector of DNA binding domain from tTA and two transcription activation modules from VP16.
RDB01583 pAxCMtTA
Shuttle vector to generate rAd expressing E. coli Tet-inducible tTA gene
RDB01584 pAxTetZ
Shuttle vector to generate rAd expressing tet-inducible lacZ
RDB01708 pAx1-RxNtTA
Recombinant adenoviral vector expressing NtTA
RDB01709 pAx1-Tet NtTA
Shuttle vector to generate rAd expressing NtTA
RDB01710 pAx1-CA-NtTA
Shuttle vector to generate rAd expressing NtTA
RDB01711 pAx1-CM-NtTA
Shuttle vector to generate rAd expressing NtTA
RDB06012 pAdex TRE-SwaI
Shuttle vector to generate rAd with Tet-responsive element (TRE) promoter
RDB01574 AxTetZ
Recombinant adenovirus expressing Tet-inducible lacZ gene
RDB01730 Ax1-RxNtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal
RDB01731 Ax1-Tet NtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal
RDB01732 Ax1-CA-NtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal
RDB01733 Ax1 CM-NtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal
RDB02351 Ax1 CM-NtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal

Luciferase

Catalog no. Name of resource Type
RDB03988 pCMVluc+
Expression vector of firefly luciferase
firefly
RDB06411 pGL3-RSV
Expression vector of firefly luciferase, RSV promoter
firefly
RDB03989 pCAluc+
Expression vector of firefly luciferase
firefly
RDB02443 pCAcc-Luc+
Expressing luciferase under the control of CAG promoter
firefly
RDB02444 pLRICALuc+
Shuttle vector to generate recombinant adenovirus
firefly
RDB02968 pTcf7wtluc
Reporter plasmid carring TCF-binding consensus sequence
firefly
RDB02969 pTcf7mtluc
Reporter plasmid carring TCF-binding consensus sequence
firefly
RDB07031 ptk-luc
Reporter construct with luciferase expression under tk promoter
firefly
RDB07032 pFGF4tk-luc
Reporter construct with luciferase expression under FGF4 and tk promoter
firefly
RDB02453 AxCA Luc+
Recombinant adenovirus expressing luciferase gene
firefly
RDB04026 pKM2L
Reporter construct with Renilla luciferase gene
Renilla
RDB05549 pKM2L-pvTK
HSV herpes simplex virus thymidine kinase (HSV-TK)
Renilla
RDB05550 pKM2L-pvSV40
SV40 simian virus 40 early enhancer/promoter region (SV40)
Renilla
RDB05551 pKM2L-pvCMV
CMV cytomegalovirus immediate enhancer/promoter (CMV)
Renilla
RDB06408 pRL-CMV mut1
Expression vector of Renilla luciferase, mutated CMV promoter
Renilla
RDB06409 pRL-CMV mut2
Expression vector of Renilla luciferase, mutated CMV promoter
Renilla
RDB06410 pRL-RSV
Expression vector of Renilla luciferase, RSV promoter
Renilla

 

LacZ

Catalog no. Name of resource
RDB06072 pCMFlag_lacZ
Expression vector of lacZ
RDB07081 pCMFlag_lacZ
Expression vector of lacZ
RDB01676 pxCANLacZ
A plasmid expressing a beta-galactosidase tagged with a NLS
RDB01870 pCAZ 2
E. coli LacZ genomic DNA
RDB02037 pLCAZ2
LacZ clone
RDB01680 pCALNLZ
A control plasmid to introduce LacZ gene into mammalian chromosome
RDB03380 pAxCAiLacZit
Shuttle vector to generate rAd harbouring E. coli LacZ
RDB02104 pAxCAZ3
Shuttle vector to generate rAd expressing E. coli lacZ
RDB01589 pAxCEAprZ
Shuttle vector to generate rAd expressing E. coli lacZ gene driven by CEA promoter
RDB07061 pAxEFFlag_LacZit2
Shuttle vector to generate recombinant adenovirus
RDB01699 pRx nZ ires Neo
Nls (nuclear localization signal), lacZ, ires, neo
RDB02976 pMFGlacZ
Shuttle vector to generate recombinant retrovirus cexpressing E. coli lacZ
RDB02039 pAxPSZ
Shuttle vector to generate rAd expressing E. coli lacZ
RDB01590 pAxRxZ
“Shuttle vector to generate rAd expressing E. coli lacZ gene driven by Rx promoter
RDB01503 pAACAZ
AAV vector expressing E. coli lacZ
RDB02038 AxPSZ
Recombinant adenovirus expressing E. coli lacZ regulated by human PSA promoter
RDB02726 AxCALacZ
Recombinant adenovirus expressing beta-galactosidase under the control of CAG promoter
RDB01750 AxCALNLNZ
Recombinant adenovirus expressing E. coli lacZ
RDB03211 AxCANLacZ
Recombinant adenovirus expressing E. coli lacZ
RDB02036 AxCAZ2-F/K20
Recombinant adenovirus expressing lacZ regulated by CAG promoter. Fiber-mutant, F/K20
RDB02196 AxCAZ3
Recombinant adenovirus expressing E. coli lacZ regulated by CAG promoter
RDB02172 AxCAZ3 F/asK21MSH beta
Recombinant adenovirus expressing E. coli lacZ. Fiber-mutant, F/asK21MSH beta
RDB02175 AxCAZ3 F/gsK21MSH beta
Recombinant adenovirus expressing E. coli lacZ. Fiber-mutant, F/gsK21MSH beta
RDB02170 AxCAZ3 F/gsMSH beta
Recombinant adenovirus expressing E. coli lacZ. Fiber-mutant, F/gsMSH beta
RDB02258 AxCAZ3-F/540S
Recombinant adenovirus expressing E. coli lacZ
RDB02240 AxCAZ3-F/asAT
Recombinant adenovirus expressing E. coli lacZ
RDB02230 AxCAZ3-F/asGRP
Recombinant adenovirus expressing E. coli lacZ
RDB02231 AxCAZ3-F/asGRP (4th)
Recombinant adenovirus expressing E. coli lacZ
RDB04375 AxCAZ3-F/asGRP27
Recombinant adenovirus expressing E. coli lacZ
RDB04376 AxCAZ3-F/asGRP27NM
Recombinant adenovirus expressing E. coli lacZ
RDB02165 AxCAZ3-F/asMSH alpha
Recombinant adenovirus expressing lacZ. Fiber-mutant, F/asMSH alpha mutant
RDB02236 AxCAZ3-F/asSec
Recombinant adenovirus expressing E. coli lacZ
RDB02237 AxCAZ3-F/asSec (4th)
Recombinant adenovirus expressing E. coli lacZ
RDB02194 AxCAZ3-F/RGD
Recombinant adenovirus expressing E. coli lacZ. Fiber-mutant, F/RGD
RDB03937 AxCAZ3-F40STAA
Recombinant adenovirus expressing E.coli lacZ
RDB03954 Ax3CAZ3-F405hiRGD
Recombinant adenovirus expressing E. coli lacZ
RDB03931 Ax3CAZ3-F40SthiRGD
Recombinant adenovirus expressing E. coli lacZ

 

(GRP0037e 2004.08.22 T.M.)

2018.11.16