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Empty Backbone for Mammalian Cell Experiment

  • Plasmid/cosmid

Catalog num.
Clone Name
Epitope TagSelectable MarkerCloningPromoterBacterial Resistance
FlagHA2xHAT72xT7Myc2xMycw/oCMVCAGEF-1RSVAmp
RDB05956
pCMV_S-FLAG
       NeoRestriction Enzyme   
RDB06083
pRSV_S-FLAG
       NeoRestriction Enzyme   
RDB07396
pCMV_S-FLAGc
       NeoGateway(R)   
RDB07397
pRSV_s-FLAGc
       NeoGateway(R)   
RDB02136
S-HA-pRc/CMV
       NeoRestriction Enzyme   
RDB02139
D-HA-pRc/CMV
       NeoRestriction Enzyme   
RDB02137
S-T7-pRc/CMV
       NeoRestriction Enzyme   
RDB02138
D-T7-pRc/CMV
       NeoRestriction Enzyme   
RDB02140
S-Myc-pRc/CMV
       NeoRestriction Enzyme   
RDB02141
D-Myc-pRc/CMV
       NeoRestriction Enzyme   
RDB07939
pEF-BOS
       noneRestriction Enzyme   
RDB07940
pEF-BOS-EX
       noneRestriction Enzyme   
RDB08938
pCAGGS
       noneRestriction Enzyme   
RDB07099
pPyCAG-BstXI-IP
       PuromycinRestriction Enzyme   
RDB12107
pPyCAG-BstXI-IB
       BlasticidinRestriction Enzyme   
RDB07100
pPyCAG-cHA-IP
       PuromycinRestriction Enzyme   
RDB07101
pBRPyCAG-cHA-IP
       PuromycinRestriction Enzyme   
RDB07105
pBRPyCAG-cHA-IN
       NeoRestriction Enzyme   

    Catalog num.Clone nameDescriptionClass.Seq file
    RDB01379pH2RneoExpression vector with simian virus 40 (SV40) early promoterVector 
    RDB01674pCALNLwA cassette to introduce cDNA into mammalian chromosomeVector 
    RDB01679pCALwLA cassette plasmid to insert cDNA into downstream CAG promoterVector 
    RDB01862pCALNL5A cassette to introduce cDNA into mammalian chromosomeVector 
    RDB01968pBMSAExpression vector, inducible promoterVector 
    RDB02546pxCAGExpression vector driven by CAG promoterVector 
    RDB03043pMNSMRetrovirus expression vector in eukaryotic cellsVector 

  • pCMV_tag vectors (en)

Empty Backbone for Recombinant Virus

  • Adenovirus

  • RIKEN BRC DNA Bank provides hundreds of shuttle vectors to generate recombinant adenovirus. We also provide vectors to construct shuttle vector for recombinant adenovirus. A list of the vectors is available at
    http://dna.brc.riken.jp/rvd/allavector.html

    Vectors to construct shuttle vector for recombinant adenovirus.
    Pdf file is available.



  • Retrovirus

  • Catalog no.Name of vectorShort titleClassification
    RDB01699pRx nZ ires NeoNls (nuclear localization signal), lacZ, ires, neoVector

Empty Backbone for Insect Cell Experiment

Catalog num.Clone nameDescriptionClass.
RDB08531pCoPUROCopia promoter expression vector of puromycin resistant gene for Schneider 2 (S2) Drosophila cells.Plasmid
RDB08532pMT-PUROMetametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells.Plasmid
RDB12152pMT-PURO2Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cellsPlasmid
RDB09004pMT-PURO2GMetametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cellsPlasmid
RDB09005pMT-PURO2RMetametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cellsPlasmid

Empty Backbone and Host Strains for Microbial Experiment

Empty Backbone

Bacterial host

  • Bacillus stearothermophilus
      RDB00139K1041Host strain of Bacillus stearothermophilus for pSTE33.
  • The host E. coli strains, RFzero-iy strain and B-95.deltaA strain that can produce recombinant proteins incorporating synthetic amino acids by reassignment of the UAG codon.
    • The RFzero-iy strains are designated to incorporate 3-iodotyrosine into produced proteins. For the incorporation, the strain is transformed with the expression plasmid in which a codon for the target tyrosine is replaced with UAG codon and cultured in 3-iodotyrosine containing medium (Mukai, T. et al., 2011). Two strains based on the BW25113 and BL-21(DE3) are available.
    • The B-95.deltaA strains can be assigned their UAG codon to synthetic amino acids by the introduction of plasmids carrying specific pair of UAG-reading tRNA and an aminoacyl-tRNA synthetase (aaRS) variant*. Increased productivity of sulfonated hirudin which is expected to improve the inhibition of blood coagulation was reported (Mukai, T. et al., 2015). The original and the growth improved derivative strains are available.
    • Reference
      Mukai, T. et al., Biochem. Biophys. Res. Commun. 411 (4): 757-761, 2011. [PMID 21782790]
      Mukai, T. et al., Sci. Rep. 5: 9699, 2015. [PMID 25982672]
      Incorporation of synthetic amino acids into proteins at specific sites
    • RDB13711B95. delta AEscherichia coli BL-21(DE3)-based host strain with no specific assignment of the UAG codon.
      RDB13712B95. delta A delta fabREscherichia coli BL21(DE3)-based host strain with no specific assignment of the UAG codon.
      RDB14427BW25113-based RFzero-iyEscherichia coli host strain to produce non-natural amino acids containing proteins.
      RDB14428BL21(DE3)-based RFzero-iyEscherichia coli host strain to produce non-natural amino acids containing proteins.
    • *Plamids that introduce synthetic amino acids are provided by RIKEN CLST.

Gene Disruption


(T.M. 2010.04.24)

2017.07.25