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Empty Backbone for Microbial Experiment


Gene Disruption


Empty Backbone for Mammalian Cell Experiment

  • Plasmid/cosmid

  • RDBName of cloneRunning titleClassSeq file
    1379pH2RneoExpression vector with simian virus 40 (SV40) early promoterVector 
    1674pCALNLwA cassette to introduce cDNA into mammalian chromosomeVector 
    1679pCALwLA cassette plasmid to insert cDNA into downstream CAG promoterVector 
    1862pCALNL5A cassette to introduce cDNA into mammalian chromosomeVector 
    1968pBMSAExpression vector, inducible promoterVector 
    2478pCEAGpolyAmammalian expression vector driven by human CEA promoterVector 
    2546pxCAGExpression vector driven by CAG promoterVector 
    3043pMNSMRetrovirus expression vector in eukaryotic cellsVector 

  • pCMV_tag vectors (en)
  • RDBName of cloneRunning titleClassSeq file
    2136S-HA-pRc/CMVExpressing HA-tagged fusion protein, CMV promoterVectorRDB2136.pdf
    2137S-T7-pRc/CMVExpressing T7-tagged fusion protein, CMV promoterVectorRDB2137.pdf
    2138D-T7-pRc/CMVExpressing a fusion protein tagged with duplicated T7, CMV promoterVectorRDB2138.pdf
    2139D-HA-pRc/CMVExpressing a fusion protein tagged with duplicated HA, CMV promoterVectorRDB2139.pdf
    2140S-Myc-pRc/CMVExpressing Myc-tagged fusion protein, CMV promoterVectorRDB2140.pdf
    2141D-Myc-pRc/CMVExpressing a fusion protein tagged with duplicated Myc epitopes, CMV promoterVectorRDB2141.pdf
    5954pCMV_S-MycExpressing Myc-tagged fusion protein, CMV promoterVectorRDB5954.pdf
    5955pCMV_D-MycExpressing a fusion protein tagged with duplicated Myc epitopes, CMV promoterVectorRDB5955.pdf
    5956pCMV_S-FLAGExpressing FLAG-tagged fusion protein, CMV promoterVectorRDB5956.pdf
    Sequence of MCS (pdf)

Empty Backbone for Recombinant Virus

  • Adenovirus

  • RIKEN BRC DNA Bank provides hundreds of shuttle vectors to generate recombinant adenovirus. We also provide vectors to construct shuttle vector for recombinant adenovirus. A list of the vectors is available at
    http://dna.brc.riken.jp/rvd/allavector.html

    Vectors to construct shuttle vector for recombinant adenovirus.
    Pdf file is available.



  • Retrovirus

  • RDB no.Name of vectorShort titleClassification
    1699pRx nZ ires NeoNls (nuclear localization signal), lacZ, ires, neoVector

(T.M. 2010.04.24)

2014.03.11