Clone Search

Keywords

Resources for

Clontech Laboratories, Inc. and RIKEN BioResource Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

Clones & Vectors for Gene Expression
  Promoter collection
  Epitope tag fusion clones
  SEREX cDNA clone
  HLA antigen cDNA clone
  Nakamura & White RFLP clone

Resources for Gene Analysis
  Cultured microbe
  Cultured animal cell
  Experimental animal
  in vitro experiment
  Plant gene resources

Clone Set & Library
  Genomic clone
  cDNA clone
  Expression clone
  Libraries
  Others

Recombinant Virus
  Recombinant Adenovirus
  Shuttle Vector for Recombinant Virus

Genomic DNA
  JCM Microbial Genomic DNA
  BRC Mouse Genomic DNA

Search for resources
  Key word search
  Depositors List
  Gene Set Collection

Empty Vectors


Empty Backbone for Mammalian Cell Experiment

  • Plasmid/cosmid

 

Catalog num.
Clone Name
Epitope Tag Selectable Marker Cloning Promoter Bacterial Resistance
Flag HA 2xHA T7 2xT7 Myc 2xMyc w/o CMV CAG EF-1 RSV Amp
RDB05956
pCMV_S-FLAG
Neo Restriction Enzyme
RDB06083
pRSV_S-FLAG
Neo Restriction Enzyme
RDB07396
pCMV_S-FLAGc
Neo Gateway(R)
RDB07397
pRSV_s-FLAGc
Neo Gateway(R)
RDB02136
S-HA-pRc/CMV
Neo Restriction Enzyme
RDB02139
D-HA-pRc/CMV
Neo Restriction Enzyme
RDB02137
S-T7-pRc/CMV
Neo Restriction Enzyme
RDB02138
D-T7-pRc/CMV
Neo Restriction Enzyme
RDB02140
S-Myc-pRc/CMV
Neo Restriction Enzyme
RDB02141
D-Myc-pRc/CMV
Neo Restriction Enzyme
RDB07939
pEF-BOS
none Restriction Enzyme
RDB07940
pEF-BOS-EX
none Restriction Enzyme
RDB08938
pCAGGS
none Restriction Enzyme
RDB07099
pPyCAG-BstXI-IP
Puromycin Restriction Enzyme
RDB12107
pPyCAG-BstXI-IB
Blasticidin Restriction Enzyme
RDB07100
pPyCAG-cHA-IP
Puromycin Restriction Enzyme
RDB07101
pBRPyCAG-cHA-IP
Puromycin Restriction Enzyme
RDB07105
pBRPyCAG-cHA-IN
Neo Restriction Enzyme

 

Catalog num. Clone name Description Class. Seq file
RDB01379 pH2Rneo Expression vector with simian virus 40 (SV40) early promoter Vector
RDB01674 pCALNLw A cassette to introduce cDNA into mammalian chromosome Vector
RDB01679 pCALwL A cassette plasmid to insert cDNA into downstream CAG promoter Vector
RDB01862 pCALNL5 A cassette to introduce cDNA into mammalian chromosome Vector
RDB01968 pBMSA Expression vector, inducible promoter Vector
RDB02546 pxCAG Expression vector driven by CAG promoter Vector
RDB03043 pMNSM Retrovirus expression vector in eukaryotic cells Vector

 

Empty Backbone for Recombinant Virus

  • Adenovirus
     
    RIKEN BRC DNA Bank provides hundreds of shuttle vectors to generate recombinant adenovirus. We also provide vectors to construct shuttle vector for recombinant adenovirus. A list of the vectors is available at
    http://dna.brc.riken.jp/rvd/allavector.html
     
    Vectors to construct shuttle vector for recombinant adenovirus.
    Pdf file is available. 

 

  • Retrovirus
     

    Catalog no. Name of vector Short title Classification
    RDB01699 pRx nZ ires Neo Nls (nuclear localization signal), lacZ, ires, neo Vector

Empty Backbone for Insect Cell Experiment

Catalog num. Clone name Description Class.
RDB08531 pCoPURO Copia promoter expression vector of puromycin resistant gene for Schneider 2 (S2) Drosophila cells. Plasmid
RDB08532 pMT-PURO Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells. Plasmid
RDB12152 pMT-PURO2 Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells Plasmid
RDB09004 pMT-PURO2G Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells Plasmid
RDB09005 pMT-PURO2R Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells Plasmid

 

Empty Backbone and Host Strains for Microbial Experiment

Empty Backbone

  • E. coli
    Catalog num. Clone name Form Registance (E. coli) Cloning Promoter Tag
    Amp Tet Cm lac T7 FLAG His GST
    RDB01911 Lambda GEX5 Phage Restriction Enzyme
    RDB06084 pETEG5 Plasmid Restriction Enzyme
    RDB06211 pT7Tet1 Plasmid Restriction Enzyme
    RDB06212 pT7Tet6 Plasmid Restriction Enzyme
    RDB07041 pETHFFc Plasmid Gateway®
    RDB07050 pETF Plasmid Restriction Enzyme
    RDB07051 pT7FCam Plasmid Restriction Enzyme

Bacterial host

  • Bacillus stearothermophilus
    RDB00139 K1041 Host strain of Bacillus stearothermophilus for pSTE33.
  • The host E. coli strains, RFzero-iy strain and B-95.deltaA strain that can produce recombinant proteins incorporating synthetic amino acids by reassignment of the UAG codon.
    • The RFzero-iy strains are designated to incorporate 3-iodotyrosine into produced proteins. For the incorporation, the strain is transformed with the expression plasmid in which a codon for the target tyrosine is replaced with UAG codon and cultured in 3-iodotyrosine containing medium (Mukai, T. et al., 2011). Two strains based on the BW25113 and BL-21(DE3) are available.
    • The B-95.deltaA strains can be assigned their UAG codon to synthetic amino acids by the introduction of plasmids carrying specific pair of UAG-reading tRNA and an aminoacyl-tRNA synthetase (aaRS) variant*. Increased productivity of sulfonated hirudin which is expected to improve the inhibition of blood coagulation was reported (Mukai, T. et al., 2015). The original and the growth improved derivative strains are available.
    • Reference
      Mukai, T. et al., Biochem. Biophys. Res. Commun. 411 (4): 757-761, 2011. [PMID 21782790]
      Mukai, T. et al., Sci. Rep. 5: 9699, 2015. [PMID 25982672]
      Incorporation of synthetic amino acids into proteins at specific sites

      RDB13711 B95. delta A Escherichia coli BL-21(DE3)-based host strain with no specific assignment of the UAG codon.
      RDB13712 B95. delta A delta fabR Escherichia coli BL21(DE3)-based host strain with no specific assignment of the UAG codon.
      RDB14427 BW25113-based RFzero-iy Escherichia coli host strain to produce non-natural amino acids containing proteins.
      RDB14428 BL21(DE3)-based RFzero-iy Escherichia coli host strain to produce non-natural amino acids containing proteins.
    • *Plamids that introduce synthetic amino acids are provided by RIKEN CLST.

 

Gene Disruption

 

(T.M. 2010.04.24)

2017.07.25