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Clontech Laboratories, Inc. and RIKEN BioResource Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

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Empty Vectors


Empty Backbone for Mammalian Cell Experiment

Mammalian expression vector (empty backbone)

Catalog num. Clone name
Description
Epitope tag Antibiotic/visual marker Promoter Cloning Antibiotics (bacteria)
RDB14213 pcDNA3.1-myc-GFP
Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells.
myc-GFP Neo CMV Restriction enzyme Amp
RDB14401 pCIneoEGFPC2
Plasmid vector for constructing GFP-tagged expression vector.
EGFP Neo CMV Restriction enzyme Amp
RDB05956 pCMV_S-FLAG
Expressing FLAG-tagged fusion protein, CMV promoter.
FLAG Neo CMV Restriction enzyme Amp
RDB06083 pRSV_S-FLAG
Expressing FLAG-tagged fusion protein, RSV promoter.
FLAG Neo RSV Restriction enzyme Amp
RDB07396 pCMV_S-FLAGc
Destination vector to generate eukaryotic expression vector, with FLAG-tag.
FLAG Neo CMV Gateway(R) Amp
RDB07397 pRSV_s-FLAGc
Destination vector to generate eukaryotic expression vector, with FLAG-tag.
FLAG Neo RSV Gateway(R) Amp
RDB11390 pPyCAG-cHA-IB
Expression vector with CAG promoter, HA-tag, Blasticidin S selection (Bsr).
HA Bsr CAG Restriction enzyme Amp
RDB10461 pBRHCAG-cHA-pA
Expression vector with CAG promoter, HA-tag, hygromycin selection (hph).
HA Hygro CAG Restriction enzyme Amp
RDB02136 S-HA-pRc/CMV
Expressing HA-tagged fusion protein, CMV promoter.
HA Neo CMV Restriction enzyme Amp
RDB02139 D-HA-pRc/CMV
Expressing a fusion protein tagged with duplicated HA, CMV promoter.
2xHA Neo CMV Restriction enzyme Amp
RDB07105 pBRPyCAG-cHA-IN
Expression vector with CAG promoter, HA-tag, Neoycin selection.
HA Neo CAG Restriction enzyme Amp
RDB11391 pPyCAG-cHA-IN
Expression vector with CAG promoter, HA-tag, Neomycin selection.
HA Neo CAG Restriction enzyme Amp
RDB12114 pPyCAG-cHA-IpacflexEGFP
Expression vector with CAG promoter, HA-tag, Puromycin selection. GFP marker.
HA Puro & EGFP CAG Restriction enzyme Amp
RDB07101 pBRPyCAG-cHA-IP
Expression vector with CAG promoter, HA-tag, Puromycin selection.
HA Puro CAG Restriction enzyme Amp
RDB07100 pPyCAG-cHA-IP
Expression vector with CAG promoter, HA-tag, Puromycin selection.
HA Puro CAG Restriction enzyme Amp
RDB10479 pPyPGK-cHA-IP
Expression vector with mouse PGK promoter, HA-tag, IRESpuro.
HA Puro PGK Restriction enzyme Amp
RDB10460 pBRPyCAG-cHA-IP
Expression vector with CAG promoter, HA-tag, Puromycin selection.
HA Puro CAG Restriction enzyme Amp
RDB10435 pBR-CAG-cHA-pA
Expression vector with CAG promoter, HA-tag.
HA n.a. CAG Restriction enzyme Amp
RDB02140 S-Myc-pRc/CMV
Expressing Myc-tagged fusion protein, CMV promoter.
Myc Neo CMV Restriction enzyme Amp
RDB02141 D-Myc-pRc/CMV
Expressing a fusion protein tagged with duplicated Myc epitopes, CMV promoter.
2xMyc Neo CMV Restriction enzyme Amp
RDB02137 S-T7-pRc/CMV
Expressing T7-tagged fusion protein, CMV promoter.
T7 Neo CMV Restriction enzyme Amp
RDB02138 D-T7-pRc/CMV
Expressing a fusion protein tagged with duplicated T7, CMV promoter.
2xT7 Neo CMV Restriction enzyme Amp
RDB12113 pPyCAG-BstXI-ZeoP2AEGFP
Expression vector with CAG promoter, HA-tag, Bleomycin (Zeo) selection. GFP marker.
n.a. Bleo(Zeo) & EGFP CAG Restriction enzyme(BstXI) Amp
RDB09315 pPyCAG-IRESzeocinpA (+SVori)
Expression vector with CAG promoter, IRES-zeo.
n.a. Bleo(Zeo) CAG Restriction enzyme (BstXI) Amp
RDB09297 pZPCAGGS
Expression vector with CAG promoter, Zeo marker.
n.a. Bleo(Zeo) CAG Restriction enzyme (BstXI) Amp
RDB12107 pPyCAG-BstXI-IB
Mammalian expression vector. Blasticidin resistance.
n.a. Bsr CAG Restriction enzyme(BstXI) Amp
RDB12112 pPyCAG-BstXI-IHisDflexEGFP
Expression vector with CAG promoter, HA-tag, HisD selection. GFP marker.
n.a. HisD & EGFP CAG Restriction enzyme(BstXI) Amp
RDB10322 pPyCAG-BstXI-IRSHisDpA
Expression vector of IRES-HisD with CAG promoter.
n.a. HisD CAG Restriction enzyme (BstXI) Amp
RDB12108 pPyCAG-BstXI-HygP2AEGFP
Expression vector with CAG promoter, HA-tag, Hygromycin selection. GFP marker.
n.a. Hygro & EGFP CAG Restriction enzyme(BstXI) Amp
RDB09233 pPyCAG-BstXI-IREShygropA
Expression vector of CAG promoter, IRES-hygromycin resistance.
n.a. Hygro CAG Restriction enzyme(BstXI) Amp
RDB09694 pCAGGSneo-delta-EcoRI
Expression vector with CAG promoter and b-globin poly(A) (with SV40 ori), neo marker.
n.a. Neo CAG Restriction enzyme Amp
RDB07099 pPyCAG-BstXI-IP
Mammalian expression vector. Blasticidin resistance.
n.a. Puro CAG Restriction enzyme(BstXI) Amp
RDB08938 pCAGGS
Mammalian expression vector with CAG promoter.
n.a. n.a. CAG Restriction enzyme Amp
RDB07939 pEF-BOS
Mammalian expression vector with human EF-1a.
n.a. n.a. EF-1 alpha Restriction enzyme Amp
RDB07940 pEF-BOS-EX
Mammalian expression vector with human EF-1a.
n.a. n.a. EF-1 alpha Restriction enzyme Amp

 

Flurescent protein expression

Catalog num. Clone name
Description
Antibiotic/visual marker Promoter Antibiotics (bacteria)
RDB10118 pmPGKpacECFPpA
Expression vector of pac-ECFP with mPGK promoter.
Puro & ECFP CAG Amp
RDB06071 pCMFlag_EGFP
Expression vector of GFP, with FLAG-tag, control vector.
FLAG-EGFP CMV Amp
RDB07071 pdxEFFlag_EGFPit2
Expression vector of EGFP.
FLAG-EGFP EF-1 alpha Amp
RDB07073 pdxEFFlag_EGFPit2
Expression vector of EGFP.
FLAG-EGFP EF-1 alpha Amp
RDB07631 pCAEGFP
Expression vector of GFP driven by CA promoter.
EGFP CAG Amp
RDB08525 pPyCAG-EGFP-IP
Expression clone of GFP under the control of CAG promoter, puromycin resistance.
Puro & EGFP CAG Amp
RDB08526 pPyCAG-EGFP-IZ
Expression clone of GFP under the control of CAG promoter, zeocin resistance.
Bleo(Zeo) & EGFP CAG Amp
RDB08527 pPyCAG-EGFP-ID
Expression clone of GFP under the control of CAG promoter, HisD resistance.
HisD & EGFP CAG Amp
RDB08528 pPyCAG-EGFP-IB
Expression clone of GFP under the control of CAG promoter, Blasticidin resistance.
Bsr & EGFP CAG Amp
RDB08529 pBRPyCAG-EGFP-IH
Expression clone of GFP under the control of CAG promoter, hygromycin resistance
Hygro & EGFP CAG Amp
RDB08530 pBRPyCAG-EGFP-IN
Expression clone of GFP under the control of CAG promoter, neomycin resistance
Neo & EGFP CAG Amp
RDB03006 pCAEGFP
Expression vectoe of jellyfish GFP
EGFP CAG Amp
RDB03956 pCAEGFP
Expression vector of EGFP.
EGFP CAG Amp

 

Vector for marker knock-in (genome editing)

Catalog num. Clone name
Description
Epitope tag Antiviotics marker Antibiotics (bacterial)
RDB13925 pMK282 (mCherry2-Bsr)
A plasmid for construction of C-terminal mCherry tagging donors.
mCherry2-Bsr Bsr Amp
RDB13924 pMK281 (mCherry2-Hygro)
A plasmid for construction of C-terminal mCherry tagging donors.
mCherry2-Hygro Hygro Amp
RDB13923 pMK280 (mCherry2-NeoR)
A plasmid for construction of C-terminal mCherry tagging donors.
mCherry2-NeoR Neo Amp
RDB13922 pMK279 (mClover-Bsr)
A plasmid for construction of C-terminal mClover tagging donors.
mClover-Bsr Bsr Amp
RDB13921 pMK278 (mClover-Hygro)
A plasmid for construction of C-terminal mClover tagging donors.
mClover-Hygro Hygro Amp
RDB13920 pMK277 (mClover-NeoR)
A plasmid for construction of C-terminal mClover tagging donors.
mClover-Neo Neo Amp
RDB13928 pMK285 (Stag-3FLAG-Bsr)
A plasmid for construction of C-terminal Stag-3FLAG tagging donors.
Stag-3FLAG-Bsr Bsr Amp
RDB13927 pMK284 (Stag-FLAG-Hygro)
A plasmid for construction of C-terminal Stag-3FLAG tagging donors.
Stag-FLAG-Hygro Hygro Amp
RDB13926 pMK283 (Stag-3FLAG-Neo)
A plasmid for construction of C-terminal Stag-3FLAG tagging donors.
Stag-3FLAG-Neo Neo Amp

 

Catalog num. Clone name Description
RDB01379 pH2Rneo Expression vector with simian virus 40 (SV40) early promoter
RDB01674 pCALNLw A cassette to introduce cDNA into mammalian chromosome
RDB01679 pCALwL A cassette plasmid to insert cDNA into downstream CAG promoter
RDB01862 pCALNL5 A cassette to introduce cDNA into mammalian chromosome
RDB01968 pBMSA Expression vector, inducible promoter
RDB02546 pxCAG Expression vector driven by CAG promoter

 

Adenovirus

  • RIKEN BRC DNA Bank provides hundreds of shuttle vectors to generate recombinant adenovirus. We also provide vectors to construct shuttle vector for recombinant adenovirus.
  • A list of the vectors is available [link]
  •  

Retrovirus

RDB no. Antibiotic/visual marker Promoter Cloning Antibiotics (bacteria)
RDB01699 pRx nZ ires Neo
Nls (nuclear localization signal), lacZ, ires, neo
lacZ-Neo CMV-retroviral LTR n.a. Amp
RDB03985 pRxhrGFP
Retroviral vector of a humanized recombinant GFP, hrGFP.
GFP CMV-retroviral LTR Restriction enzyme Amp
RDB03043 pMNSM
Retrovirus expression vector in eukaryotic cells.
Neo Retroviral LTR Restriction enzyme Amp

 

Empty Backbone for Insect Cell Experiment

Catalog num. Clone name
Description
Epitope tag Antibiotic/visual marker Cloning Promoter Bacterial resistance
RDB08532 pMT-PURO
Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells.
His-V5 Puro Restriction enzyme MT Amp
RDB12152 pMT-PURO2
Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells
His-V5 Puro Restriction enzyme MT Amp
RDB09004 pMT-PURO2G
Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells
His-V5 Puro & EGFP Restriction enzyme MT Amp
RDB09005 pMT-PURO2R
Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells
His-V5 Puro& DsRed Restriction enzyme MT Amp
RDB08531 pCoPURO
Copia promoter expression vector of puromycin resistant gene for Schneider 2 (S2) Drosophila cells.
n.a. Puro n.a. copia Amp
RDB08035 pUAST-GFPN-lacZ
P-element mediated gene transfer vector of GFP-N and lacZ.
n.a. GFP-lacZ n.a. Gal4-UAS Amp

 

Empty Backbone and Host Strains for Microbial Experiment

E. coli empty Backbone

Catalog num. Clone name Form Registance (E. coli) Cloning Promoter Tag
Amp Tet Cm lac T7 FLAG His GST
RDB01911 Lambda GEX5 Phage Restriction Enzyme
RDB06084 pETEG5 Plasmid Restriction Enzyme
RDB06211 pT7Tet1 Plasmid Restriction Enzyme
RDB06212 pT7Tet6 Plasmid Restriction Enzyme
RDB07041 pETHFFc Plasmid GatewayR
RDB07050 pETF Plasmid Restriction Enzyme
RDB07051 pT7FCam Plasmid Restriction Enzyme

Yeast empty Backbone

Bacillus empty Backbone

RDB00919 pSTE12 Bacillus stearothermophilus shuttle vector
RDB00920 pSTE33 Bacillus stearothermophilus shuttle vector

Bacillus stearothermophilus bacterial host

RDB00139 K1041 Host strain of Bacillus stearothermophilus for pSTE33.

E. coli bacterial host

  • The host E. coli strains, RFzero-iy strain and B-95.deltaA strain that can produce recombinant proteins incorporating synthetic amino acids by reassignment of the UAG codon.
  • The RFzero-iy strains are designated to incorporate 3-iodotyrosine into produced proteins. For the incorporation, the strain is transformed with the expression plasmid in which a codon for the target tyrosine is replaced with UAG codon and cultured in 3-iodotyrosine containing medium (Mukai, T. et al., 2011). Two strains based on the BW25113 and BL-21(DE3) are available.
  • The B-95.deltaA strains can be assigned their UAG codon to synthetic amino acids by the introduction of plasmids carrying specific pair of UAG-reading tRNA and an aminoacyl-tRNA synthetase (aaRS) variant*. Increased productivity of sulfonated hirudin which is expected to improve the inhibition of blood coagulation was reported (Mukai, T. et al., 2015). The original and the growth improved derivative strains are available.
  • Reference
    Mukai, T. et al., Biochem. Biophys. Res. Commun. 411 (4): 757-761, 2011. [PMID 21782790]
    Mukai, T. et al., Sci. Rep. 5: 9699, 2015. [PMID 25982672]
    Incorporation of synthetic amino acids into proteins at specific sites

    RDB13711 B95. delta A Escherichia coli BL-21(DE3)-based host strain with no specific assignment of the UAG codon.
    RDB13712 B95. delta A delta fabR Escherichia coli BL21(DE3)-based host strain with no specific assignment of the UAG codon.
    RDB14427 BW25113-based RFzero-iy Escherichia coli host strain to produce non-natural amino acids containing proteins.
    RDB14428 BL21(DE3)-based RFzero-iy Escherichia coli host strain to produce non-natural amino acids containing proteins.
  • *Plamids that introduce synthetic amino acids are provided by RIKEN CLST.

 

(GRP0048e 2010.04.24 T.M.)

2017.11.23