Gene Engineering Division - DNA BANK - > pCMV_tag and pRSV_tag assay-ready clones

pCMV_tag and pRSV_tag assay-ready clones

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The Gene Engineering Division provides full CDS expression clones that were constructed
by our division, and the confirmed entire nucleotide sequences of each clone and their
expression proteins determined by the western blotting and the immunofluorescent staining.
This collection contains human and other animal ORF (open reading frame).

1. Distribution of clones

1.1 Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER must state an acknowledgement to the RIKEN BRC which is participating in the National Bio-Resource Project of the MEXT, Japan.

1.2 How to obtain clones?

How to obtain clones 

pRSV_S-FLAG expression clones

catalog no. Name of cloneRunning titlePDF
RDB06071 pCMFlag_EGFP Expression vector of GFP, with FLAG-tag, control vector ​
RDB06072 pCMFlag_lacZ Expression vector of lacZ, with FLAG-tag, control vector ​
RDB06297 pCMFlag_hsNR0B1 Expression vector of human ACH ​
RDB06299 pCMFlag_hsNR5A1 Expression vector of human AD4BP
RDB06296 pCMFlag_hsADR Expression vector of human ADR
RDB07028 pCMFlag_hsAKT2 Expression vector of human AKT2
RDB06251 pCMFlag_hsATF1 Expression vector of human ATF1
RDB06257 pCMFlag_hsATF2 Expression vector of human ATF2
RDB06252 pCMFlag_hsBACH2 Expression vector of human BACH2
RDB06605 pCMFlag_hsBAD Expression vector of human BAD
RDB06253 pCMFlag_hsBATF Expression vector of human BATF
RDB06597 pCMFlag_hsBIRC5 Expression vector of human BIRC5/API4 ​
RDB06665 pCMFlag_hsCCNA2 Expression vector of human CCNA2/Cyclin A2
RDB06415 pCMFlag_hsCCNB2 Expression vector of human CCNB2
RDB06416 pCMFlag_hsCCNH Expression vector of human CCNH
RDB06062 pCMFlag_hsDDIT3 Expression vector of human CHOP10
RDB07012 pCMFlag_hsCREB2 Expression vector of human CREB2
RDB06108 pCMFlag_hsCREB3 Expression vector of human CREB3
RDB06267 pCMFlag_hsCREB3L1 Expression vector of human CREB3L1/Oasis
RDB06258 pCMFlag_hsCREB3L2 Expression vector of human CREB3L2
RDB06412 pCMFlag_hsCREBL1 Expression vector of human CREBL1
RDB06256 pCMFlag_hsCEBPE Expression vector of human CEBPE
RDB06259 pCMFlag_hsDBP Expression vector of human DBP
RDB07016 pCMFlag_hsE2F8 Expression vector of human E2F8
RDB06063 pCMFlag_hsNFIL3 Expression vector of human E4BP4
RDB06374 pCMFlag_hsELK1 Expression vector of human ELK1
RDB07020 pCMFlag_hsELK4 Expression vector of human ELK4
RDB06295 pCMFlag_hsESR2 Expression vector of human ER beta
RDB06260 pCMFlag_hsESRRA Expression vector of human ESRRA
RDB06752 pCMFlag_hsETS2 Expression vector of human ETS2
RDB07270 pCMFlag_hsETV4 Expression vector of human PEA3
RDB06375 pCMFlag_hsFLI1 Expression vector of human FLI1
RDB06064 pCMFlag_hsFOS Expression vector of human FOS
RDB06261 pCMFlag_hsFOSL1 Expression vector of human FOSL1
RDB06065 pCMFlag_hsFOSL2 Expression vector of human Fra2 (FOSL2)
RDB06753 pCMFlag_hsGATA1 Expression vector of human GATA1
RDB06376 pCMFlag_hsGATA3 Expression vector of human GATA3
RDB06109 pCMFlag_hsGPE1BP Expression vector of human GPE1BP
RDB06377 pCMFlag_hsHDAC6 Expression vector of human HDAC6 ​
RDB06378 pCMFlag_hsHIF1A Expression vector of human HIF1A
RDB06110 pCMFlag_hsHLF Expression vector of human HLF
RDB06666 pCMFlag_hsIL1B Expression vector of human IL1B/IL-1 beta
RDB06998 pCMFlag_hsIL6 Expression vector of human IL6
RDB06667 pCMFlag_hsILF2 Expression vector of human ILF2/NF-AT, 45kDa
RDB06250 pCMFlag_hsJDP2 Expression vector of human JDP2
RDB06254 pCMFlag_hsJUN Expression vector of human JUN
RDB06670 pCMFlag_hsKlf4 Expression vector of human KLF4
RDB06379 pCMFlag_hsKLK3 Expression vector of human KLK3
RDB06602 pCMFlag_hsLIN28 Expression vector of human LIN28
RDB06066 pCMFlag_hsNR1H2 Expression vector of human LXR beta
RDB06264 pCMFlag_hsMAFB Expression vector of human MAFB
RDB06413 pCMFlag_hsMAFG Expression vector of human MAFG
RDB06067 pCMFlag_hsMAFK Expression vector of human MAFK
RDB06671 pCMFlag_hsc-Myc Expression vector of human c-Myc
RDB07029 pCMFlag_BvMYC Expression vector of bovine MYC
RDB07026 pCMFlag_hsL-Myctv3 Expression vector of human MYCL1
RDB06601 pCMFlag_hsNanog Expression vector of human NANOG
RDB07027 pCMFlag_hsNCAMtv1 Expression vector of human NCAMtv1
RDB06111 pCMFlag_hsNFE2 Expression vector of human NFE2
RDB06265 pCMFlag_hsNFE2L2 Expression vector of human NFE2L2
RDB07018 pCMFlag_hsNFKB1 Expression vector of human NFKB1
RDB07017 pCMFlag_hsNFKB2 Expression vector of human NFKB2
RDB06668 pCMFlag_hsNFKBIA Expression vector of human NFKBIA/IkappaB alpha
RDB06269 pCMFlag_hsNR1D2 Expression vector of human NR1D2
RDB06270 pCMFlag_hsNR1D1 Expression vector of human NR1D1
RDB06262 pCMFlag_hsNR1H4 Expression vector of human NR1H4
RDB06255 pCMFlag_hsNR1I3 Expression vector of human NR1I3
RDB06372 pCMFlag_hsNR6A1 Expression vector of human NR6A1
RDB07013 pCMFlag_hsNRF1 Expression vector of human NRF1
RDB07014 pCMFlag_hsNRF1 Expression vector of human NRF1
RDB07015 pCMFlag_hsNRF3 Expression vector of human NRF3
RDB06266 pCMFlag_hsNRL Expression vector of human NRL
RDB06999 pCMFlag_hsNUDT21 Expression vector of human NUDT21
RDB06298 pCMFlag_hsNR4A2 Expression vector of human NURR1 ​
RDB06598 pCMFlag_hsOct3/4isoform1 Expression vector of human Oct3/4 isofom 1
RDB06669 pCMFlag_hsOct3/4isoform2 Expression vector of human Oct3/4 isofom 2
RDB06268 pCMFlag_hsPPARA Expression vector of human PPARA
RDB06068 pCMFlag_hsPPARD Expression vector of human PPARD/PPAR delta
RDB06380 pCMFlag_hsPTEN Expression vector of human PTEN
RDB06414 pCMFlag_hsRARA Expression vector of human RARA
RDB06373 pCMFlag_hsRORA Expression vector of human RORA
RDB06069 pCMFlag_hsRORB Expression vector of human ROR beta
RDB06112 pCMFlag_hsRXR alpha Expression vector of human RXR alpha coding sequence
RDB06751 pCMFlag_hsRXR beta Expression vector of human RXR beta coding sequence
RDB06113 pCMFlag_hsSHP Expression vector of human SHP
RDB07019 pCMFlag_hsSMAD3 Expression vector of human SMAD3
RDB06600 pCMFlag_hsSox2 Expression vector of human SOX2
RDB07271 pCMFlag_hsSOX11 Expression vector of human SRY (sex determining region Y)-box 11
RDB06381 pCMFlag_hsSTAT1 Expression vector of human STAT1 ​
RDB06382 pCMFlag_hsSTAT5A Expression vector of human STAT5A ​
RDB06383 pCMFlag_hsSTAT6 Expression vector of human STAT6 ​
RDB06271 pCMFlag_hsTEF Expression vector of human TEF
RDB06300 pCMFlag_hsTHRA Expression vector of human THR alpha ​
RDB06604 pCMFlag_hsTHOC4 Expression vector of human THOC4/ALY
RDB06114 pCMFlag_hsTR4 Expression vector of human TR4
RDB06115 pCMFlag_hsTR4 variant Expression vector of human TR4 variant hTAK1
RDB06070 pCMFlag_hsTHRB Expression vector of human TR beta (ErbA-beta)
RDB06272 pCMFlag_hsXBP1 Expression vector of human XBP1
RDB06263 pCMFlag_hsZF Expression vector of human ZF

Transfection with cloned DNA

Transfection solution A
Lipofectamine 2000 (Invitrogen) 2 ul
OptiMEMI (Invitrogen) 50 ul
Transfection solution B
DNA 0.8 ug
OptiMEMI 50 ul
Procedure of transfection
  1. Prepare 90% confluent Cos-1 cells in 24-well plate.
  2. Mix Transfection solution A and B.
  3. Replace culture medium with the mixture of Transfection solution.
  4. Add 100 ul of culture medium after 1 hour incubation.
  5. Replace the mixture with culture medium after 6 hours incubation.

Detection of FLAG tag by the Western blotting

RIPA Buffer
NP-40 1%
sodium deoxycholate 0.25%
EDTA (pH 8.0) 1 mM
tris-HCl (pH 7.8) 50 mM
NaCl 150 mM
Protease inhibitors
PMSF 1 mM
leupeptin 1 ug/ml
pepstatin A 1 ug/ml
2x sample buffer, SDS-PAGE
tris-HCl (pH 6.8) 1 mM
SDS 4%
glycerol 20%
beta-mercaptoethanol 10%
bromophenol blue 0.002%
Antibodies
anti-FLAG M2 mAb Sigma, F-3165
anti-mouse IgG-hrp MBL

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(2007.12.05. T.M.)

2015.10.25



 

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