RIKEN BioResource Center DNA Bank Mail News

http://dna.brc.riken.jp/en/newsen.html

:: CONTENT ::::::::::::::::::::::::::::::::
- Revision of the Distribution Fees for the Bioresources
- Genome Network Project Human Full-Length cDNA Clone is now
available
- B6N Mouse BAC clone is now available
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- Revision of the Distribution Fees for the Bioresources
http://www.brc.riken.jp/inf/en/distribute/fee.shtml#dna
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The RIKEN BioResource Center (BRC) is a non-profit institution funded by the Japanese Government. We are engaged in collecting, maintaining, multiplying, storing and distributing bioresources, in order to support research and development in the field of life sciences. The RIKEN BRC has been providing bioresources not only to scientists in Japan but also in other countries with minimum fees solely to reimburse the RIKEN BRC for a portion of the preparation and handling costs of the requested bioresources. Due to release of new bioresources and changes in governmental policies, it has become necessary to revise the distribution fees.

Orders of 20 tubes or more of Clone and host are eligible for a volume discount. For details see
http://www.brc.riken.jp/inf/en/distribute/dnafee.shtml.

We sincerely hope that these revisions will not cause too much inconvenience our customers. We appreciate your understanding and cooperation. We are looking forward to receiving your requests for our bioresources.

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- Genome Network Project Human Full-Length cDNA Clone is now
available
http://www.brc.riken.jp/lab/dna/en/GNPcloneen.html
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The RIKEN BioResource Center is distributing copies of full-length human cDNA clones, produced and organized through the MEXT Genome Network Project to academia under its distribution policy.

There are two types of cDNA clone collections.
1) Human Full-Length cDNA clones
Approximately 30,000 clones corresponding to 60% of all human genes (14,000 genes) produced and organized by Dr. Sumio Sugano of the University of Tokyo and Dr. Yoshihide Hayashizaki of RIKEN OSC.
2) Human Gateway® Entry clones
Approximately 50,000 clones corresponding to 6,300 genes produced by cloning PCR-amplified open reading frame fragments from full-length human cDNA clones and studied through the government-supported MEXT Genome Network Project. DNA fragments cloned into Gateway® entry clones can be transferred into one or more destination vectors simultaneously using Gateway® technology. The Gateway® Entry clones include clones made from cDNA created by the Research Association for Biotechnology under the New Energy and Industrial Technology Development Organization Full-length Human cDNA Sequencing Project.

Results of the project attained using the Genome Network Project cDNA clones have appeared in journals including Nature Genetics. Detailed information on these clones is available via the Genome Network Platform (http://genomenetwork.nig.ac.jp/).

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- B6N Mouse BAC clone is now available
http://www.brc.riken.jp/lab/dna/en/NBRPB6Nbacen.html
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The Gene Engineering Division has constructed a BAC library of the B6N substrain and the RIKEN BioResource Center completed BAC end sequencing of B6N and register the sequence data with DDBJ in a collaboration with the National Institute of Genetics under the Genome Information Upgrading Program of MEXT NBRP. The B6N Mouse BAC library consisted of 62,000 clones representing an estimated coverage of 77% haploid genome.

A new database, 'Mouse BAC Brower' is available at the site of the National Institute of Genetics (http://analysis2.lab.nig.ac.jp/mouseBrowser/). This database provides information of genomic locations of the B6N Mouse BAC clones on a B6J's genome sequence as well as the context of other features, such as genes.

Using the database, researchers can be find BAC clones in silico with direct querying (ex. gene symbol, name of gene product, gene ID and so on).

If you are interested in our BAC clones, please feel free to contact us.

The germ cell competent C57BL/6N ES cell lines are available from the Cell Engineering Division of RIKEN BRC.
http://www.brc.riken.jp/lab/animal/mailnews/nm200909_02.html

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2010.04.20