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Evaluation of chaperone mediated autophagy (CMA) activity by the GAPDH-HT indicator. (2017/06/29 N.N.)

  • "GAPDH-HT indicator" is a CMA marker consisting of HaloTag and GAPDH which is a typical substrate of CMA. The CMA activity in the cells can be evaluated by using GAPDH-HT indicator and an appropriate under the fluorescent-labeled HaloTag ligand.
  • Reference
    Seki, T. et al. Establishment of a novel fluorescence-based method to evaluate chaperone-mediated autophagy in a single neuron. PLoS One 7 (2): e31232, 2012. PMID: 22363588
    Sato, M. et al. Fluorescent-based evaluation of chaperone-mediated autophagy and microautophagy activities in cultured cells. Genes Cells 21 (8): 861-873, 2016. PMID: 27377049
  • DNA Resource
    GAPDH-HT/pcDNA5/FRT (cat # RDB15088)

Establishment of deficient cells using Cre recombinase and floxed mouse derived cells (2017/06/23 T.M.)

  • Has2 deficient cells were established by using Cre recombinase expressing AxCANCre adenovirus. Mouse Has2 gene floxed allele cells were segregated from the transgenic mice having the exon 2 flanked by two loxP sites. And then the cells were infected with AxCANCre adenovirus carrying the Cre recombinase to remove the exon 2.
  • Reference
    Chanmee, T. et al. Hyaluronan production regulates metabolic and cancer stem-like properties of breast cancer cells via hexosamine biosynthetic pathway-coupled HIF-1 signaling. J. Biol. Chem. 291 (46): 24105-24120, 2016. PMID: 27758869.
  • DNA Resource
    AxCANCre (cat# RDB01748)

M-INK, a novel tool for tagging the mature melanosomes.(2017/05/02 N.N.)

G-CaMP, a fluorescent calcium sensor.(2017/03/16 N.N.)

  • A Fluorescent probes "G-CaMP" for measuring intracellular calcium concentration are available.
  • The G-CaMPs are calcium sensor proteins consisting of Calmodulin (CaM) calcium-binding region, M13 fragment of myosin light chain kinase and fluorescent protein. In the original paper, G-CaMPs were used to observe cardiomyocytes differentiated from iPS cells (Shiba, Y. et al, 2016) and neurons of zebrafish (Muto, A. et al, 2011).
  • Reference
    • Shiba, Y. et al. Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Nature, 538 (7625): 388-391, 2016. PMID: 27723741
    • Ohkura, M. et al. Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One, 7 (12): e51286, 2012. PMID: 23240011
    • Ohkura, M. et al. An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials. PLoS One, 7 (7): e39933, 2012. PMID: 22808076
    • Muto, A. et al. Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish. Proc. Natl. Acad. Sci. U S A., 108 (13): 5425-5430, 2011. PMID: 21383146
  • DNA Resource
    G-CaMP4.1 (cat.# RDB14606)
    G-CaMP-HS (cat.# RDB14607)
    G-CaMP6 (cat.# RDB14609)
    G-CaMP7 (cat.# RDB14610)
    G-CaMP8 (cat.# RDB14611)
    G-CaMP7.09 (cat.# RDB14612)

    Please also visit Calcium Ion Sensor Clones.
Construction of reporter gene having long promoter by using BAC clone and with recombineering technique.(2017/02/28 T.M.)
  • Luciferase reporter assay is a powerful tool for real-time monitoring of gene expression in living cells. It is recommended to construct reporter gene with lager promoter but hard to do so by means of common cloning technique. In this article, 20 kb promoter of Hprt gene was retrieved and cloned into a luciferase reporter by using BAC clone and with recombineering technique.
  • Reference
    Endo, T. et al. Evaluation of an Hprt-luciferase reporter gene on a mammalian artificial chromosome in response to cytotoxicity. Yonago Acta Med. 59 (2): 174-182 PMID: 27493490.
  • DNA Resource
    C57BL/6N (B6N) mouse BAC clone
    Mouse B6N BAC clone B6Ng01-126E09 (Clone search results)

A fluorescent probe to evaluate activity of autophagy. (2017/02/21 N.N.)
  • Plasmid clone of a novel probe capable of evaluating the activity of autophagy by GFP/RFP signal ratio has arrived.
  • Autophagy in the mouse embryonic fibroblasts, HeLa cells and the zebrafish embryo was observed in the original paper.
  • Reference
    Kaizuka, T. et al. An Autophagic Flux Probe that Releases an Internal Control. Mol. Cell, 64 (4): 835-849, 2016. PMID: 27818143.
  • Deposited Resource

Green- and Red-emitting luciferases with luminescence.(2017/2/2 T.M.)

Histac-H3K9/K14 for visualization of acetylation activity of histone H3 in cells.(2016/9/27 T.M.)
  • Reference
    Nakaoka, S., Sasaki, K., Ito, A., Nakao, Y., Yoshida, M. A Genetically Encoded FRET Probe to Detect Intranucleosomal Histone H3K9 or H3K14 Acetylation Using BRD4, a BET Family Member. ACS Chem. Biol. 11 (3): 729-733, 2016. PMID: 25946208
  • DNA Resource
    1. pcDNA3.1(+)-Histac-H3K9/K14 (catalog#RDB14340)
GimRET for visualization of protein concentration in cells.(2016/9/13 T.M.) Nakanori that specifically binds to a complex of sphingomyelin and cholesterol.(2016/9/13 T.M.) Fluorescent Ubiquitination-based Cell Cycle Indicator (Fucci) by single plasmid.(2016/6/20 T.M.)
  • Fucci2a cell cycle phase markers allow visualization and estimation of cell cycle progress by observation of green and red fluorescent proteins. This plasmid express two distinct colored fluorescent proteins, one of which fused to Cdt1 degron, another one of which fused to Geminin degron. Therefore, the transfectant indicate distinct color fluorescence between the cell (G1) and the cell (S/G2/ M).
  • Reference
    Mort, R.L., Ford, M.J., Sakaue-Sawano, A., Lindstrom, N.O., Casadio, A., Douglas, A.T., Keighren, M.A., Hohenstein, P., Miyawaki, A., Jackson, I.J. Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696, 2014.

Mutation analyses of genes on 6p12-p11 in patients with juvenile myoclonic epilepsy. (2016/4/12 K.N.)
  • Authors had been narrowed down to 3.5 cM size on a region of short arm of chromosome 6 (6p12-p11) as the genomic region (EJM1) related to juvenile myoclonic epilepsy (JME).
    In this paper, they explore to find genes critical to the symptom. First of all physical mapping of 3.5 cM EJM1 was done. Then based on the map, 14 genes in EJM1 from patient family and from healthy family was analyzed. In the process, several YAC clones provided from our division was analyzed by STS-PCR and used for the reference to line up the BAC/PAC contig (assembled).
  • Reference
    Suzuki, T., Delgado-Escueta, A.V., Alonso, M.E., Morita, R., Okamura, N., Sugimoto, Y., Bai, D., Medina, M.T., Bailey, J.N., Rasmussen, A., Ramos-Peek, J., Cordova, S., Rubio-Donnadieu, F., Ochoa, A., Jara-Prado, A., Inazawa, J., Yamakawa, K. Mutation analyses of genes on 6p12-p11 in patients with juvenile myoclonic epilepsy. Neurosci. Lett. 405 (1-2): 126-131, 2006.

Genius method to get rid of the target protein with human culture cells (2016.03.25 K.N.)

A novel transgenic mouse model carrying human Tribbles related protein 3 (TRB3) gene and its site specific phenotype. (2016/3/11 T.Y.)
  • Authers had been demonstrated that TRB3 pseudokinase activates cancer cells and also induces nuclear enlargement. To elucidate human-TRB3-induced-mouse-liver-tissue phenotype, They constructed transgenic mice, which has conditional inducible TRB3 by Cre-loxP system. To do so, pCALNL5 (RDB01862) and pxCANCre (RDB01675) provided from our lab were used.
  • Reference
    Sakai, Y., Fukamachi, K., Futakuchi, M., Miyoshi, I., Tsuda, H., Suzui, M., Hayashi, H. A novel transgenic mouse model carrying human Tribbles related protein 3 (TRB3) gene and its site specific phenotype. Biol. Pharm. Bull. 37 (6): 1068-1074, 2014.

Aggregation of ALS-linked FUS mutant sequesters RNA binding proteins and impairs RNA granules formation. (2016/2/4 M.O.)
  • Authers had been demonstrated that nomal RNA granules (P-body) formation is perturbed by FUS/TLS mutant protein aggregation induced by its deficient NLS. In this study, they observed P-body dynamycs in living cell cultured various condition using GFP-DCP1 expression vector, which constructed using DCP1 cDNA clone (IRAL010J08) provided from our lab.
  • Reference
    Takanashi, K., Yamaguchi, A., Aggregation of ALS-linked FUS mutant sequesters RNA binding proteins and impairs RNA granules formation. Biochem. Biophys. Res. Commun. 452 (3): 600-607, 2014.

Cas9-poly(A) expressing improved plasmid (2016.01.22 T.M.)

  • Chromatin remodelling and autocrine TNF alpha are required for optimal interleukin-6 expression in activated human neutrophils. (2015/12/18 T.Y.)
    • To elucidate the regulatory mechanism of IL6 expression in human neurophils, authors examined the transcription regulation of upstream region of IL6 gene a part by a luciferase reporter assay. The constructs for this assay were delivated from pGL4-phIL6 RDB07313.
    • Reference
      Zimmermann, M., Aguilera, F.B., Castellucci, M., Rossato, M., Costa, S., Lunardi, C., Ostuni, R., Girolomoni, G., Natoli, G., Bazzoni, F., Tamassia, N., Cassatella, M.A. Chromatin remodelling and autocrine TNF alpha are required for optimal interleukin-6 expression in activated human neutrophils. Nat. Commun. 6: 6061, 2015.

  • Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma.(2015/10/23 K.N.)
    • The authors found several genes including LIN28 indicates elevated expression-level in SP cells of the oral squamous cell carcinoma. They also found that the overexpression of LIN28 gene (RDB06602) in the oral squamous cell carcinoma causes aggressive cell activities in terms of proliferation, colony formation and invasion into a solid medium. RDB05956 was used as a control vector.
    • Reference
      Hayashi S, Tanaka J, Okada S, Isobe T, Yamamoto G, Yasuhara R, Irie T, Akiyama C, Kohno Y, Tachikawa T, Mishima K. Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma. Exp. Cell Res. 319 (8) :1220-1228, 2013.

  • Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells (2015/7/31 T.Y)
    • To elucidate the role of the Notch signaling pathway on human mesenchymal stem cells (KP-hMSCs) to oligodendrocyte differentiation, the KP-hMSCs were transfected with a dominant negative RBP-J (R218H) (cat# RDB03021) to compromise Notch1 function and examined the expression level of differentiation markers. WT-RBP-J plasmid clone (cat# RDB03022) was used as control.
    • Reference
      Lee YJ, Hung SC, Chu MS. Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells. Stem Cells Cloning 3:165-173, 2010.

  • Dimerization of Sir3 via its C-terminal winged helix domain is essential for yeast heterochromatin formation. (2015/7/17 Y.K)
    • Sir3, a factor of gene silencing in budding yeast contains wH (winged helix-turn-helix domain) domain at C-terminal domain. Authors elucidate a structure and a function of wH domain of Sir3. IRAK013J04 (cat# RDB07566: Human Orc1 clone) was used to subclone its wH domain in E. coli expression vector, and the recombinant protein was compared to Sir3 wH domain.
    • Reference
      Oppikofer M, Kueng S, Keusch JJ, Hassler M, Ladurner AG, Gut H, Gasser SM. Dimerization of Sir3 via its C-terminal winged helix domain is essential for yeast heterochromatin formation. EMBO J. 32 (3): 437-449, 2013

  • WDR81 is necessary for purkinje and photoreceptor cell survival. (2015/7/4 M.O)
    • A mutant mouse strain nur5 shows tremor, an abnomal gait, Purkinje cell degeneration and photoreceptor cell loss as its phenotype. To indicate that WDR81 gene as the responsible gene for nur5 mouse strain, a BAC clone MSMg01-261K04 was used.
    • Reference
      Traka M, Millen KJ, Collins D, Elbaz B, Kidd GJ, Gomez CM, Popko B. WDR81 is necessary for purkinje and photoreceptor cell survival. J Neurosci. 33 (16): 6834-6844, 2013.

  • Surface plasmon resonance-biosensor detects the diversity of responses against epidermal growth factor in various carcinoma cell lines. (2015/6/19 T.Y)
    • Surface plasmon resonance (SPR) – biosensor detects change of angle of resonance (AR) and hence intracellular signaling is monitored. To study the mechanisms of AR change along with activation of EGFR, wild type and mutant EGFR were expressed in cultured cells. To construct expression vectors, pco12 EGFR (cat# RDB01276) was used.
    • Reference
      Hiragun T, Yanase Y, Kose K, Kawaguchi T, Uchida K, Tanaka S, Hide M. Surface plasmon resonance-biosensor detects the diversity of responses against epidermal growth factor in various carcinoma cell lines. Biosens Bioelectron. 32 (1) : 202-207, 2012

  • Vectors for specific expressed in cancer cells. (2015/1/23 K.N.)
    • The HSV-TK gene in pTK5 plasmid was used to construct recombinant adenovirus having AFR promoter for the restricted expression of thymidine kinase in cancer cells.
    • Reference
      Kurayoshi K, et al., Biochem Biophys Res Commun. 450 (1): 240-246 (2014).

Resource Information and Announcements


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A novel gene involved in germ cell formation (2017.02.01 T.M.)

Genetic contamination test kit for mouse (2017.02.01 T.M.)

Congratulations to Dr. Yoshinori Ohsumi!! (2016.10.03 T.M.)

  • The 2016 Nobel Prize in Physiology or Medicine is awarded to Dr. Yoshinori Ohsumi for his discoveries of mechanisms for autophagy.

Plasmid clones to study autophagy are available. (2016.10.03 T.M.)

The Fifth RIKEN BRC-Nanjing University MARC Mouse Resource Workshop 2016 (2016.05.11 T.M.)

Draft genome sequencing of more than 120 strains of eukaryotic microbes (Japanese text only) (2016/03/01).

Genome sequencing of JCM strains under the NBRP program (Eukaryotic microbes) (2016/02/29).

DsRed2 and mCherry for academic use (2016.02.17 T.M.)

Prepayment of distribution fees (overseas organizations only) (2015.12.05)

[RIKEN BRC] DNA Bank Mail News 2015.11.04.en (2015.11.04)

  • Lecture and demonstration for handling recombinant adenovirus
  • The Nobel Prize in Chemistry 2015
  • The 44th Annual Meeting of the Japanese Society for Immunology
  • BMB2015 Biochemistry and Molecular Biology

Award (2015.10.23)

Genes involved in the DNA repair (2015.10.07 T.M.)

    Genetic resources for genes involved in the DNA repair are available.
    Looking forward to receiving your request. [Click here]

[RIKEN BRC] DNA Bank Mail News 2015.09.10.en (2015.09.10)

  • The 74th Annual Meeting of the Japanese Cancer Association
  • Pick Up Resource
  • Histac for visualization of acetylation of the Histon H4 -
  • Focused Resource - Resources used in a recent paper -
  • Genomic locus annotated BAC clones

Getting BioResources through the PubMed! (2015.07.10 T.M.)

  • The PubMed is not only for searching research articles.
  • Please click on the "LinkOut - more resources" link following Abstract section. "NBRP resources -National BioResource Project" in the expanded list of "Research materials" provides you a link to a resouce catalog as well as research articles. [Click here!]

The 7th Asian Network of Research Resource Centers (ANRRC) International Meeting (2015.07.08, T.M.)

[RIKEN BRC] DNA Bank Mail News 2015.03.18.en (2015.03.18)

  • The 2015 Annual Conference of the Japan Society for Bioscience, Biotechnology and Agrochemistry
  • Pick Up Resource - AID system -
  • Focused Resource - Reference of materials -
  • Link between KEGG and our genetic materials by the LinkDB

Perspectives: Banking on biological tools for research innovation (2014.09.27)

  • The biodiversity of the natural world is an unrivaled source of inspiration and learning. Scientists in the many branches of the life sciences have spent endless hours studying many of the millions of species... >>more

Quality Examination and Information Release Policy on Our Bioresources (2014.09.01)

RIKEN BRC Seminar: Recombinant Adenovirus, July 18 (2014.07.04)

  • The 122nd RIKEN BRC SEMINAR
    Date, July 18, 2014
    Time, 16:00-
    Location, Moriwaki-Kazuo Hall, Tsukuba campus
    Language, Japanese

  • Speaker 1
    Saki Kondo, PhD
    Laboratory of Molecular Genetics,
    The Institute of Medical Science, The University of Tokyo
    Improved helper-dependent (HD) adenovirus vectors capable of carrying 30kb DNA and practical issues for establishing supply system

  • Speaker 2
    Izumu Saito, M.D,. Ph.D
    Laboratory of Molecular Genetics,
    The Institute of Medical Science, The University of Tokyo Supply and feedback of frontier adenovirus vectors: switching of cell- specific expression, increase of shRNA effect and possible application to CRISPR system
    The 122nd RIKEN BRC SEMINAR (Language, in Japanese)

The Third RIKEN BRC/Nanjing University MARC International Summer Intensive Course of the Mouse (link) (2014.05.09)

Link between KEGG and our genetic materials (2014.05.03)

    ♦ The KEGG (Kyoto Encyclopedia of Genes and Genomes) database of pathways and orthologs among human, mouse and fission yeast are now linked to our clones and can be searched by users. Click here!

Revision of Distribution Fees for the Bioresources.

    ♦ Due to changes in prices and protocols since the last revision of our fees, it has become necessary to revise them again.
    Revision of Distribution Fees on April 1, 2014.

The 2013 Nobel Prize in Physiology or Medicine

    ♦ Genetic resources for regulation of vesicle traffic are available.
    Looking forward to receiving your request.
    Please visit here for resources.

Brachypodium distachyon (experimental plant of monocot)

ANRRC 2013 -The 5th ANRRC International Meeting-

[RIKEN BRC] DNA Bank Mail News Aug. 09, 2013

  • Closure of all operations at RIKEN BioResource Center for three days
  • Luciferase clones to study signal cascades
  • The expression clones of endo-type cellulase
  • Distribution of common marmoset (Callithrix jacchus) EST Clone

Common marmoset EST clone is now available

Dr. Kazuo Moriwaki Awarded by MEXT Minister

Gene-constructed Recombinant Adenovirus!

  • More than 250 recombinant adenoviruses are available.
    They can be used for the infection of cultured mammalian cells as well as of living mice.
    For example, Kumadaki, S. et al. [J. Biol. Chem., 286 (47): 40835-40846 (2011)] reported that Cre recombinase expressed by adenovirus AxCANCre (catalog # RDB01748) was used for the restricted gene disruption in the liver cells of KLF5 floxed mice.
    We also provide HEK293 cell (catalog # RCB1637) for production and amplification of recombinant adenovirus and HeLa cell (catalog # RCB0007) for detection of replication competent adenovirus (RCA).
    Recombinant Adenovirus

Genomic DNA - Be free from cultivation and breading!!

Revision of Distribution Fees for the Bioresources

Expression vectors for mammalian cells

    ♦ We provide the expression-confirmed clones of human transcription factors (bZIP and nuclear hormone receptor). Empty expression vectors under the control of CMV promoter carrying T7-, HA-, Myc- (Dr. Yoshihiro Takemoto, DNA Cell Biol. 16: 893-896, 1997) or FLAG-tag are also available.
    Please visit here for datail.

Monitoring transfection efficiency by GFP expression clone

    ♦ We recommend a GFP expression clone pCMFlag_EGFP (cat # RDB 6071) to monitor the efficiency of transfection in mammalian cells. Mix pCMFlag_EGFP with your expression clones, transfect mammalian cells with them, and then count the number of GFP-positive cells under the fluorescent microscope. We are looking forward to sending the pCMFlag_EGFP to you.
    Please visit here for datail.

A gene required for growth and differentiation of pluripotential cells in the mouse embryos.

    ♦ Identification of gene that promotes differentiation of pluripotential cells through the analysis of classical mouse mutant. MSM/Ms Mouse BAC Clones were used for the research.
    Please visit here for datail.

Congratulation!
Dr. Yamanaka wins Nobel Prize in Physiology or Medicine for his work in iPS cell technology.

GFP Resources

    ♦ RIKEN BioResource Center (RIKEN BRC) and GE Healthcare Bio-Sciences (GE) concluded an agreement for the transfer of bioresources containing green fluorescent protein (GFP bioresources) to investigators at academic institutions. By generosity of GE, RIKEN BRC is able to distribute the GFP bioresources to non-profit and academic researches without license fees.
    Furthermore, we are now able to distribute the GFP bioresources to for-profit entities with Bona fide Licensees of GE as of May 22, 2012.
    Please visit the following web site for more information.
    http://www.brc.riken.jp/inf/en/news/gfp_conclude.shtml
    ♦ List of GFP Bioresources
    http://dna.brc.riken.jp/en/gfp_resourceen.html

Announcement of closure of all operations at RIKEN BioResource Center and Tsukuba Institute for three days from August 20 to 22, 2012.

    ♦ We would like to inform you that RIKEN will be closed for three days from August 20 to 22, 2012 as a continuing policy for conserving electricity, energy and environment.
    ♦ RIKEN BioResource Center and Tsukuba Institute will be also closed during the same period. All our operations regarding bioresources and all clerical work will be suspended during this period.
    ♦ We apologize for any inconvenience this may cause. We would like to ask you for your patience and cooperation. All our operations will resume on Thursday, August 23.
    Thank you very much for your understanding.

    Details
    1. Period: From August 20, Monday to August 22, Wednesday, 2012
    2. Our operations to be suspended:
    ♦ Telephone inquiry and email response
    ♦ Processing of orders on bioresources
    ♦ Shipment of bioresources
    ♦ Sending quotations and invoices
    ♦ All clerical work

G-CaMP is available.

    G-CaMP, genetically-encoded fluorescent calcium sensor cDNAs encoding a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase were deposited by Dr. Jin-ichi Nakai, Brain Science Institute, RIKEN and Saitama University.

[RIKEN BRC] DNA Bank Mail News Apr. 06, 2012

  • Bioresources for the screening of useful chemicals and studying biomass utilization
  • RIKEN BRC signs GFP Transfer License with GE Healthcare Bio-Sciences
  • Report on your achievements is appreciated

p38alpha MAP kinase pathway (2012.02.18)

Biomass Collection. (2011.12.09)

    ♦ Expression plasmids and plasmid clones of cellulase gene of Trichoderma reesei is available.

GFP Resources (2011.09.16)

    ♦ RIKEN BRC signs GFP Transfer License with GE Healthcare Bio-Sciences.

NRCD Human Full-Length cDNA Clones

    ♦ NRCD Human Full-Length cDNA Clones produced and organized by Dr. Seishi Kato of the Research Institute of National Rehabilitation Center for Persons with Disabilities is available to academia under its distribution policy. (2011.08.16)

Newly deposited genetic materials. (2011.06.08)

[RIKEN BRC] DNA Bank Mail News Jun. 08, 2011

  • Suspension of operation at RIKEN BioResource Center (BRC) for three days in this summer, from August 22 to August 24, 2011
  • Newly deposited genetic materials

Suspension of operation at RIKEN BioResource Center (BRC) for three days in this summer, from August 22 to August 24, 2011. (2011.06.02)

    ♦ To cooperate with the sever shortage of electricity supply expected in this summer due to the Eastern Japan Disaster, RIKEN will close its all campuses and facilities for three days, from Monday, August 22 to Wednesday, August 24 (RIKEN's response to the Tohoku Pacific Offshore Earthquake). During the period, RIKEN BRC will also suspend its all operation, including shipments of bioresources and responses to the orders and inquiries.
    ♦ All operation at RIKEN BRC will be conducted normally until Friday, August 19 and from Thursday, August 25.
    ♦ This suspension may cause some inconvenience to our users. We sincerely ask you for your understanding and cooperation.

    RIKEN BioResource Center

C57BL/6N (B6N) BAC library (2011.03.31)

    ♦ We have just accomplished end-sequencing of the C57BL/6N (B6N) BAC library, and published the data through our web site.

Resumption of Delivery of Bioresources. (2011.03.28)

Plan for Resumption of Delivery of Bioresources. (2011.03.22)

Temporal Suspension of Delivery of Bioresources due to the Earthquake. (2011.03.14)

Newly deposited genetic materials. (2010.11.24)

[RIKEN BRC] DNA Bank Mail News Nov. 24, 2010

  • Newly deposited genetic materials
  • Please send the information on your achievement

Report of the 2nd ANRRC (Asian Network of Research Resource Centers) Meeting is updated. (2010.11.15)

List of promoter activity of Firefly Luciferase Construct is up dated (2010.10.26)

[RIKEN BRC] DNA Bank Mail News Sep. 15, 2010

  • Mammalian Expression Vectors with Epitope Tag

Depositors List is up dated (2010.09.08)

Report on your achievements is appreciated (2010.07.07)

    ♦ We appreciate the report on your achievements such as research papers and/or patents which were obtained though use of our DNA materials. Your report will be added immediately to the accompanying information of the DNA material. This is an excellent way to increase the utility value of the DNA material as well as the visibility of your research.
    ♦ Please send us the following information to dnabank@brc.riken.jp:
    1) Research Paper: First Author, Title, Name of the Journal, Volume, Pages and Published Year
    2) Patent: Holder, Title, Number and Year
    ♦ Your publication and patent information will be added to the accompanying information of the bioresource in web catalog and to the list of publication.
    http://dna.brc.riken.jp/ja/reflist.html.
    ♦ When you publish your research results in a scientific journal, please refer to the origin of materials in Materials & Methods or Acknowledge section as follows:
    ________ (name of BIOLOGICAL RESOURCE) was provided by the RIKEN BRC through the National Bio-Resource Project of the MEXT, Japan.
    ♦ Your cooperation and understanding are essential for enrichment of information on bioresource, improvement of quality of scientific research and sustainable operation of our Center.

[RIKEN BRC] DNA Bank Mail News Jul. 07, 2010

  • Report on your achievement is appreciated
  • C57BL/6N Mouse BAC clones are now available

Genome Network Project Human Full-Length cDNA Clone is now available (2010.04.20)

    ♦ The RIKEN BioResource Center is distributing copies of full-length human cDNA clones, produced and organized through the MEXT Genome Network Project to academia under its distribution policy.
    ♦ There are two types of cDNA clone collections.
    1) Human Full-Length cDNA clones
    Approximately 30,000 clones corresponding to 60% of all human genes (14,000 genes) produced and organized by Dr. Sumio Sugano of the University of Tokyo and Dr. Yoshihide Hayashizaki of RIKEN OSC.
    2) Human Gateway® Entry clones
    Approximately 50,000 clones corresponding to 6,300 genes produced by cloning PCR-amplified open reading frame fragments from full-length human cDNA clones and studied through the government-supported MEXT Genome Network Project. DNA fragments cloned into Gateway® entry clones can be transferred into one or more destination vectors simultaneously using Gateway® technology. The Gateway® Entry clones include clones made from cDNA created by the Research Association for Biotechnology under the New Energy and Industrial Technology Development Organization Full-length Human cDNA Sequencing Project.
    ♦ Results of the project attained using the Genome Network Project cDNA clones have appeared in journals including Nature Genetics. Detailed information on these clones is available via the Genome Network Platform (http://genomenetwork.nig.ac.jp/).
    More about Genome Network Project Human Full-Length cDNA Clone

[RIKEN BRC] DNA Bank Mail News Apr. 20, 2010

  • Revision of the Distribution Fees for the Bioresources
  • Genome Network Project Human Full-Length cDNA Clone is now available
  • B6N Mouse BAC clone is now available

C57BL/6N (B6N) Mouse BAC clone is now available (2010.03.01)

    ♦ The Gene Engineering Division has constructed a BAC library of the B6N substrain and the RIKEN BioResource Center completed BAC end sequencing of B6N and register the sequence data with DDBJ in a collaboration with the National Institute of Genetics under the Genome Information Upgrading Program of MEXT NBRP. The B6N Mouse BAC library consisted of 62,000 clones representing an estimated coverage of 2.6-fold and 77% haploid genome.
    ♦ A new database, 'Mouse BAC Brower' is also available at the site of the National Institute of Genetics (http://analysis2.lab.nig.ac.jp/mouseBrowser/). This database provides information of genomic locations of the B6N Mouse BAC clones on a B6J's genome sequence as well as the context of other features, such as genes. Using the database, researchers can be find BAC clones in silico with direct querying (ex. gene symbol, name of gene product, gene ID and so on).
    More about B6N BAC clone
    ♦ If you are interested in our BAC clones, please feel free to contact us.
    ♦ The germ cell competent C57BL/6N ES cell lines are available from the Cell Engineering Division of RIKEN BRC.
    http://www.brc.riken.jp/lab/animal/mailnews/nm200909_02.html

[RIKEN BRC] DNA Bank Mail News Mar. 01, 2010

  • B6N Mouse BAC clone is now available
  • Addition of data of immunofluorescent staining for the full CDS expression clones

Addition of data of immunofluorescent staining for the full CDS expression clones (2010.02.19)

    ♦ The Gene Engineering Division provides full CDS expression clones that were constructed by our division, and the confirmed entire nucleotide sequences of each clone and their expression proteins determined by the western blotting and the immunofluorescent staining.
    ♦ We opened additional data of immunofluorescent staining for cells introduced expression vectors listed below.
    ♦ If you are interested in our BAC clones, please feel free to contact us.
    More about pCMV_S-FLAG expression clones
    List of genes
    RDB 6068 human PPARD/PPAR delta
    RDB 6069 human ROR beta
    RDB 6070 human TR beta; ErbA-beta
    RDB 6108 human CREB3
    RDB 6111 human NFE2
    RDB 6295 human ER beta
    RDB 6598 human Oct3/4 isoform1
    RDB 6600 human Sox2
    RDB 6601 human Nanog homeobox
    RDB 6602 human LIN28
    RDB 6670 human Klf4
    RDB 6671 human c-Myc
    RDB 6998 human IL6
    RDB 6999 human CPSF5/CPSF25
    RDB 7012 human CREB2; ATF4
    RDB 7013 human NRF1; NFE2L1
    RDB 7014 human NRF1; NFE2L1
    RDB 7015 human NRF3; NFE2L3
    RDB 7016 human E2F8
    RDB 7017 human NFKB2
    RDB 7018 human NFKB1
    RDB 7019 human SMAD3
    RDB 7020 human Sap-1
    RDB 7026 human MYCL1, transcript variant 3
    RDB 7027 human neural cell adhesion molecule 1, transcript variant 1
    RDB 7028 human AKT2
    RDB 7029 human v-myc myelocytomatosis viral oncogene homolog (avian)
    RDB 7270 human PEA3
    RDB 7271 human SRY(sex determining region Y)-box 11

BAC Brower of Japanese macaque is released (2010.02.01)

    ♦ RIKEN DNA Bank is distributing BAC clones of Japanese macaque (Macaca fuscata fuscata).
    ♦ A new database, 'BAC Brower of Japanese macaque' is constructed by the Information Center of the National BioResource Project, National Institute of Genetics and now available (http://analysis2.lab.nig.ac.jp/jmonkeyBrowser). This database provides information of genomic locations of the Japanese macaque BAC clones on a rhesus macaque's genome sequence as well as the context of other features, such as genes.
    ♦ Using the database, researchers can be find BAC clones in silico with direct querying (ex. gene symbol, name of gene product, gene ID and so on).
    ♦ If you are interested in our BAC clones, please feel free to contact us.
    More about Japanese macaque BAC clone

Genomic DNAs form the culture collection of RIKEN BRC-JCM is available (2010.01.28)

    ♦ The RIKEN DNA Bank provides genomic DNAs prepared from microorganisms of the RIKEN BRC-JCM. The strains listed below are newly available.
    Bifidobacterium longum subsp. infantis JCM 1222T
    Clostridium paraputrificum JCM 1293T
    Clostridium pasteurianum JCM 1408T
    Corynebacterium glutamicum JCM 1318T
    Corynebacterium kroppenstedtii JCM 11950T
    Lactobacillus acidophilus JCM 1132T
    Lactobacillus gasseri JCM 1131T
    Methanocaldococcus fervens JCM 15782T
    Methanocaldococcus infernus JCM 15783T
    Methanopyrus kandleri JCM 9639T
    Mycobacterium kansasii JCM 6379T
    Nocardia asteroides JCM 3384T
    Propionibacterium acnes JCM 6425T
    Streptococcus oralis JCM 12997T
    Streptococcus salivarius subsp. thermophilus JCM 20026
    More about Microorganisms genomic DNA

New Entry of Promoter Collection (2009.12.08)

    ♦ The promoter, one of the most important regions of the gene to regulate the transcription has been collected as a large series in the DNA Bank, RIKEN BioResource Center. These promoters were subcloned into plasmid that has a firefly luciferase reporter gene as a promoter reporter construct (the Promoter Series).
    ♦ We opened and started distributing of the novel 108 promoter constructs (80 types of TP53 targeted genes and 28 types of ATF family targeted genes). All of the Promoter series were validated by restriction enzyme digestion and confirmation of end sequences of the insert DNA. Furthermore, we checked the promoter activities of the Promoter series on 3 kinds of cell lines (HeLa, HepG2 and Hep3B cells).
    ♦ The Promoter Series are very useful for individual and/or comprehensive analysis of transcriptional regulation of genes. And each construct is also available for a source of tissue specific expression of genes that you focused.
    More about Promoter Collection

Life Technologies Corporation signs license agreement with RIKEN BioResource Center (2009.11.03)

    ♦ The Life Technologies Corporation (former Invitrogen IP Holdings, Inc.) and RIKEN BioResource Center have signed a license agreement on July 2, 2009. With generosity of Life Technologies Corporation, Riken BRC is now able to receive, maintain, replicate and distribute Gateway® Entry clones and Expression clones for not-for-profit academic research under this agreement.
    ♦ The Entry clone harboring cDNA can be distributed for not-for-profit academic research without any license fees. The Expression clone harboring mammalian cDNA can be distributed from the RIKEN BRC for not-for-profit academic research with a small amount of license fee to the Life Technologies Corporation. In addition to the regular fee for a cDNA clone, users are asked to pay RIKEN BRC 1,250 YEN per clone that includes the license fee, handling of currency exchange, money transfer and other expense. Every six months, RIKEN BRC will report to Life Technologies Corporation a number of distributions of Entry and Expression clones and send a sum of license fee for Expression clones. Any commercial users of the Gateway® Entry or Expression clones must contact Life Technologies Corporation for prior written approval.
    ♦ RIKEN BRC has set the Material Transfer Agreements (MTA) for each transfer to protect the intellectual property rights of developers of bioresources and to define the responsibility of users. We have opened a path of the not-for-profit academic use of bioresources produced by using research tools owned by commercial entities.
    ♦ Deposition of bioresources to the RIKEN BRC frees researchers from cumbersome preservation and distribution of materials fellow researchers. Furthermore, deposition increases chance of collaboration and citation of research paper. Your deposition of genetic resources in particular the Gateway® clones to the Gene Engineering Division is most appreciated.

Genomic DNAs form the culture collection of RIKEN BRC-JCM is available (2009.11.03)

    ♦ The RIKEN DNA Bank provides genomic DNAs prepared from microorganisms of the RIKEN BRC-JCM. The strains listed below are newly available.
    Alcaligenes faecalis JCM 20666
    Azotobacter vinelandii JCM 21475T
    Bacteroides thetaiotaomicron JCM 5827T
    Bacillus cereus JCM 20266
    Bifidobacterium adolescentis JCM 1275T
    Bifidobacterium catenulatum JCM 1194T
    Blautia hansenii JCM 14655T
    Blautia producta JCM 1471T
    Caldanaerobacter subterraneus subsp. tengcongensis JCM 11007T
    Enterococcus faecalis JCM 20313T
    Geobacillus stearothermophilus JCM 14450
    Neisseria mucosa JCM 12992T
    Rhizobium radiobacter JCM 21034
    Streptomyces coelicolor JCM 4357T
    More about Microorganisms genomic DNA

The "Whole Cell Project Database" was updated (2009.07.06)

    ♦ RIKEN DNA Bank is distributing gene disruption plasmids and expression plasmids of Thermus thermophilus HB8 deposited by Dr. Seiki Kuramitsu, the Whole Cell Project, the SR Systembiology Reserch Group, RIKEN SPring-8 Center.
    ♦ The Whole Cell Project Database has been maintained by the SR Systembiology Reserch Group, RIKEN SPring-8 Center and provided information with the clones for expression of genes of Thermus thermophilus HB8 (http://www.srg.harima.riken.jp/h_db/index.html).
    ♦ A new version of the database is now available and researchers can be find plasmids for disruption of genes of T. thermophilus HB8.
    ♦ The gene disruptant of T. thermophils HB8 can be easily prepared by adding this plasmid into the culture medium. The target gene in this plasmid was replaced by the thermostable kanamycine resistant gene. The length of the homologous region outside of the target gene is about 500 bp (only 10 bp of the target gene are left in both sides).
    More about Disruption Plasmid of Thermus thermophilus HB8

Genomic DNAs form the culture collection of RIKEN BRC-JCM is available (2009.07.06)

    ♦ The RIKEN DNA Bank is distributing genomic DNAs prepared from microorganisms of the RIKEN BRC-JCM. The strains listed below are newly available.
    Pseudomonas aeruginosa JCM 20301
    Staphylococcus aureus subsp. aureus JCM 20624T
    More about Microorganisms genomic DNA

Genomic DNAs form the culture collection of RIKEN BRC-JCM is available (2009.06.11)

    ♦ The RIKEN DNA Bank is distributing genomic DNAs prepared from microorganisms of the RIKEN BRC-JCM. The strains listed below are newly available.
    Geobacillus stearothermophilus JCM 14450
    Caldanaerobacter subterraneus subsp. tengcongensis JCM 11007T
    Alcaligenes faecalis JCM 20666
    Azotobacter vinelandii JCM 21475T
    Bacillus cereus JCM 20266
    Streptomyces coelicolor JCM 4357T
    More about Microorganisms genomic DNA

F344 & LE BAC browser is available (2009.04.07)

    ♦ Rattus norvegicus database F344/Stm and LE/Stm BAC browser is released by NBRP Rat.
    Using this database, you may find your BAC clones.
    NBRP Rat NBRP Rat BAC Clone

Discount Service for Plasmid, Cosmid and Host strains (2009.04.01)

    ♦ For large orders of the Plasmid, Cosmid and Host strains, there is a discount depending on the total number of tubes ordered. For single orders of 20 or more tubes, the total charge will be reduced according to the number of tubes.
    More about Discount Service for Plasmid, Cosmid and Host strains

FUCCI mice for visualizing cell-cycle in vivo (2009.03.30)

    ♦ RIKEN and Amalgaam Inc. concluded a bio-genetic resource deposit agreement for launching a delivery service for Fucci mice which are transformed by a fluorescent cell cycle probe.
    For detail, visit E-MAIL News No.57
    by the Experimental Animal Division, RIKEN BioResource Center.

A mouse iPS cell line is now available (2009.03.28)

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2017.05.02