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JCM Microbial Genomic DNA

General Information

Bacteria and archaeans are cultured in the RIKEN BRC-JCM and their DNA are prepared in the Gene Engineering Division. High molecular weight DNA is prepared by phenol-chloroform extraction. The DNA is suitable for amplification by PCR. The concentration and purity of DNA samples are determined spectrophotometrically and further checked by PCR amplification of 16S rRNA gene and following electrophoresis. Nucleotide sequences are also confirmed. Shipping amout is 1 μg per vial and DNA concentrations generally range from 50 to 100 ng/μl. (The concentrations of individual samples are supplied with a data sheet.) The DNAs are stored in TE buffer (10 mM TRIS pH7.5, 1mM EDTA) at -30oC.

Attention International Transfer – “Veterinary Permission” or “Permit to Import Quarantine Material” may be required for shipment of the biological materials. Please follow guide lines in your country.

Forms for Distribution

Forms required

Forms Description
Form A Order Form
Please specify strain names with JGD numbers (catalog number).
Form C Material Transfer Agreement
Please put the terms and condisions that is indicated in the individual data sheet of the genomic DNA listed here.
(Please e-mail to to obtain MTA in MS-Word format)
Order Form (FormA) [Open a new window!!]
Please complete the form with your shipping information including your account number of an international courier (FedEx. World Courier, TNT Express, DHL Global Forwarding and others).
NOTE: User registration on our system is required before download the Order Form for credit card (Visa / Master).
Material Transfer Agreement (Category I MTA) [Word]
For the use of our bioresource in research for not-for-profit academic purpose by a non-profit organization.

We ask a signature of the Authorized Representative of a recipient institution.
In the Section 2(a), please write your purpose of use of clone in 10 to 20 words.

(Please e-mail to to obtain MTA in MS-Word format)
Material Transfer Agreement (Category II MTA) [Word]
For the use of our bioresource in research for the following cases:
 1. For research to be conducted by for-profit organizations.
 2. For collaborative research between for-profit organization and not-for-profit organization.
 3, For research by not-for- organization outsourced and sponsored by for-profit organization.
 4. For for-profit research by not-for-profit organization including R&D with the aim of patent acquisition.

Distribution fee per vial (one microgram):
17,000 YEN (For use in research for not-for-profit academic purpose).
34,000 YEN (For use in research for-profit-research purpose).
(Shipping cost is not included.)

Please visit this page for further information of distribution and fees.

If you are inquiring about the availability of a specific DNA, please give as much information about the strain stock number as possible.
For more information contact:

[Regarding DNA] [Regarding bacteria strains]
Gene Engineering Division,
RIKEN BioResource Research Center
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
Fax: +81-29-836-9120
Microbe Division / Japan Collection of Microorganisms
RIKEN BioResource Research Center
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
Fax: +81-29-836-9561

The availability of some DNAs is limited. All requests for these DNAs will be given due consideration, but we cannot assure availability.

Personal data protection policy
For protection of personal data at RIKEN BioResource Research Center, please reffer Personal data protection policy.

Related Information

Reconstitution of DNA

  • In the case you received plastic vials contains precipitated DNA with ethanol.
  • A precipitant might stick to the lid or wall during transportation.
  • Before opening the tube, please spin down a minute or two at 5,000 g (~7,000 rpm, table top microfuge).
  • Please remove ethanol with pipet (DO NOT tooch precipitate!)
  • Immediately after removal of ethanol resuspend the DNA in an appropriate amount of TE buffer (10 mM Tris-Cl, pH 7.5, 1 mM EDTA), and dissolve the DNA pellet by gently tapping with fingers (DO NOT shake vigorously! A little amount of ethanol won’t affect to many applications).
  • If the DNA is difficult to solve, leave the vial at 4 degreeC overnight to allow the DNA to dissolve.
  • Once reconstituted in TE buffer, the DNA should be stored at -30 degreeC.