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Clontech Laboratories, Inc. and RIKEN BioResource Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

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G2/M transition


Summary of G2/M transition

Cyclin B is synthesized in the end of S phase and associated with cdc2. CyclinB/cdc2 complex is held inactive during G2 phase and become active during M phase mainly because of Thr 161 phosphorylation by CyclinH/cdc7 complex, and Thr14 and Tyr15 dephosphorylation by CDC25 phoaphatase. (Contributed by Dr. Koji Nakade)


Reporters

Catalog no. Name of clone Short description
RDB13080 pCAG-Fucci2a Fucci2a cell cycle reporter.
RDB13081 pROSA-floxNeo-Fucci2a Fucci2a cell cycle reporter. Targeting vector for R26 locus.

Locus RDB no. Type Name of clone Running title
chk1 2949 DNA clone chk1+ Plasmid clone of fission yeast Chk1
Chek1 6946 H3042B08 Chek1, NIA/NIH Mouse 15K cDNA Clone (full CDS)
CDC25C 5908 DNA clone pKM2L-phCDC25C(2) Promoter Bank clone, Human cdc25C promoter
CDC25C 5841 ShuttleVector pAxit-phCDC25C-rLuc (R) Shuttle vector to generate recombinant adenovirus harboring renilla luciferase reporter.
CDC25C 5840 ShuttleVector pAxit-phCDC25C-rLuc (F) Shuttle vector to generate recombinant adenovirus harboring renilla luciferase reporter.
CDC25C 5468 DNA clone pKM2L-phCDC25C Promoter Bank clone, Human cdc25C promoter
CDC25B 2668 ShuttleVector pAxCALNLhCDC25B (reverse) Shuttle vector to produce rAd expressing human CDC25B
CDC25B 2667 ShuttleVector pAxCALNLhCDC25B (forward) Shuttle vector to produce rAd expressing human CDC25B
CDC25B 2334 ShuttleVector pAxCAhCDC25B (reverse) Shuttle vector to produce rAd expressing human CDC25B
CDC25B 2333 ShuttleVector pAxCAhCDC25B (forward) Shuttle vector to produce rAd expressing human CDC25B
CDC25B 1203 DNA clone CDC25Hu2 Human cdc 25B cDNA
CDC25A 5476 DNA clone pKM2L-phCDC25A Promoter Bank clone, Human cdc25A promoter
CDC25A 4653 ShuttleVector pAxCALNLhCDC25A(forward) Shuttle vector to generate rAd expressing human CDC25A
CDC25A 4646 ShuttleVector pAxCALNLhCDC25A(reverse) Shuttle vector to generate rAd expressing human CDC25A
cdc2 1570 DNA clone pIBI delta Xe-cdc2-33R Xenopus cdc2 (kinase-deficient) cDNA
cdc2 1569 DNA clone pIBI delta Xe-cdc2 Xenopus cdc2 cDNA
CCNH 6416 DNA clone pCMFlag_hsCCNH Expression vector of human CCNH
CCNH 4093 ShuttleVector pAxCALNLhCyclin H Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCNH 3510 ShuttleVector pAxCALNLhCyclin H(forward) Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCND3 4589 DNA clone SEREX clone NGO-Br-20 (ID 859) #1 SEREX clone NGO-Br-20 (ID 859) #1
CCNB2 6415 DNA clone pCMFlag_hsCCNB2 Expression vector of human CCNB2
CCNB2 5448 ShuttleVector pAxCALNLhCCNB2(reverse) Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB2 5447 ShuttleVector pAxCALNLhCCNB2(forward) Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB2 5430 ShuttleVector pAxCALNLhCCNB2(forward) Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB2 5425 ShuttleVector pAxCALNLhCCNB2(reverse) Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB2 5424 ShuttleVector pAxCALNLhCCNB2(forward) Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB2 5367 ShuttleVector pAxCALNLhCCNB2(reverse) Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB2 5366 ShuttleVector pAxCALNLhCCNB2(forward) Shuttle vector to generate rAd harboring CCNB2 Sugano full-length cDNA clone
CCNB2 4098 ShuttleVector pAxCALNLhCCNB2 Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCNB2 3515 ShuttleVector pAxCALNLhCCNB2(forward) Shuttle vector to generate rAd harboring Sugano full-length cDNA clone

(GSB0010e, T.M. 2004.04.07)

2014.09.12