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GFP Resources

Visualization of histone H4 acetylation

    ♦ The Histac fluorescent probes deposited by Dr. Kazuki Sasaki allow you monitoring the state of activity of acetylation of histone H4 by fluorescence in living cells.
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Knock in markers with CRISPR/Cas9 genome editing

    ♦ As a part of Auxin Inducible Degron (AID) System clones, Dr. Masato Kanemaki provides a series of knock-in fluorescent and selection markers with CRISPR/Cas9 genome editing.
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Monitoring Notch signal

    ♦ The pRBS-EGFP and RBP-J-Venus expression clones deposited by Dr. Makoto Mark Taketo and Dr. Kenji Tanigaki, respectively, allow you monitoring the state of activation of the Notch signaling by fluorescence in living cells.
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EGFP-MBD-nls expression clone

    ♦ The EGFP-MBD-nls protein recognizes the methylated DNA and you can follow status of the DNA methylation in situ under physiological conditions using the pEGFP-MBD-nls expression clone.
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pCMFlag_EGFP (cat # RDB 6071)

    ♦ We recommend a GFP expression clone pCMFlag_EGFP (cat # RDB 6071) to monitor efficiency of transfection of mammalian cells. Mix the pCMFlag_EGFP with your expression clones, transfect them to mammalian cells, and then count number of fluorescent cells under the fluorescent microscope. We look forward to serving the pCMFlag_EGFP to you.

G-CaMP calcium sensor is available

    ♦ G-CaMP, Genetically-encoded fluorescent calcium sensor cDNAs encoding a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase were deposited by Dr. Jin-ichi Nakai, the Saitama University.
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pxCALNLEYFP (cat # RDB 6232)

    ♦ Plasmid clone pxCALNLEYFP (RDB 6232) can be used for a positive control of Cre-mediated gene regulation. The pxCALNLEYFP contains CAG promoter, loxP-Neo-loxP stuffer sequence and coding region of EYFP. In this example, the pxCALNLEYFP was transfected to COS1 cells with or without pxCANCre (RDB 1675), which expresses Cre recombinase.


Expression of YFP was detected in cells transfected pxCALNLEYFP with pxCANCre (right) but not pCALNLEYFP alone (left).

GFP Resources

    ♦ RIKEN BioResource Center (RIKEN BRC) and GE Healthcare Bio-Sciences (GE) concluded an agreement for the transfer of bioresources containing green fluorescent protein (GFP bioresources) to investigators at academic institutions. By generosity of GE, RIKEN BRC is able to distribute the GFP bioresources to non-profit and academic researches without license fees.
    ♦ Furthermore, we are now able to distribute the GFP bioresources to for-profit entities with Bona fide Licensees of GE as of May 22, 2012.
    ♦ Please visit the following web site for more information.
    http://www.brc.riken.jp/inf/en/news/gfp_conclude.shtml (in English)
    http://ja.brc.riken.jp/news/gfp_conclude.shtml (in Japanese)

 

Order Forms

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List of GFP Resources

Name of cloneFormulaCat no.
pEGFP-MBD-nls
  Expression clone of methylated DNA binding domain (MBD) of human MBD1
DNA solutionRDB08344
pAxCABFP (forward)
  Shuttle vector for rAd harboring blue fluorescent protein cDNA
DNA solutionRDB03224
pAxCABFP (reverse)
  Shuttle vector for rAd harboring blue fluorescent protein cDNA
DNA solutionRDB03225
pAxCAEGFP (forward)
  Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA
DNA solutionRDB03232
pAxCAEGFP (reverse)
  Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA
DNA solutionRDB03233
pAxCAEYFP (forward)
  Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNA
DNA solutionRDB03234
pAxCALNLBFP (forward)
  Shuttle vector for rAd harboring blue fluorescent protein cDNA
DNA solutionRDB03251
pAxCALNLBFP (reverse)
  Shuttle vector for rAd harboring blue fluorescent protein cDNA
DNA solutionRDB03252
pAxCALNLEGFP (forward)
  Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA
DNA solutionRDB03259
pAxCALNLEGFP (reverse)
  Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA
DNA solutionRDB03260
pAxCALNLEYFP (forward)
  Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNA
DNA solutionRDB03261
pAxCALNLEYFP (reverse)
  Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNA
DNA solutionRDB03262
AxCAEGFP (forward)
  Recombinant adenovirus harboring enhanced green fluorescent protein cDNA
Recombinant virusRDB03343
AxCALNLEGFP (forward)
  Recombinant adenovirus harboring enhanced green fluorescent protein cDNA
Recombinant virusRDB03347
pAxCAEGFP
  Shuttle vector to generate rAd harbouring jerry fish GFP
DNA solutionRDB05815
pAxCAEGFPit2
  Shuttle vector to generate rAd harbouring jerry fish GFP
DNA solutionRDB05817
pAxEFEGFPit2
  Shuttle vector to generate rAd harbouring jerry fish GFP
DNA solutionRDB05819
pCMFlag_EGFP
  Expression vector of GFP, with FLAG-tag, control vector
DNA solutionRDB06071
pxCALNLEYFP
  Expression vector of EYFP
DNA solutionRDB06232
pAxEFFlag_EGFPit2
  Shuttle vector to generate recombinant adenovirus
DNA solutionRDB07060
pAxEFFlag_EGFPit2
  Shuttle vector to generate recombinant adenovirus
DNA solutionRDB07062
pdxEFFlag_EGFPit2
  Expression vector of EGFP
DNA solutionRDB07071
pdxEFFlag_EGFPit2
  Expression vector of EGFP
DNA solutionRDB07073
pAx1-CA-gfp
  Recombinant adenoviral vector expressing green fluorescent protein (GFP) gene
DNA solutionRDB01705
Ax1 CA gfp
  Recombinant adenovirus expressing green fluorescent protein
Recombinant virusRDB01727
pCAEGFP
  Expression vectoe of jellyfish GFP
DNA solutionRDB03006
pWEAx-CAEGFP-F/RGD
  Shuttle vector to produce rAd expressing jellyfish GFP
DNA solutionRDB03008
AxCAEGFP-F/RGD
  Recombinant adenovirus expressing jellyfish GFP
Recombinant virusRDB03010
pWEAxCAEGFP-F40STAA
  Shuttle vector to generate rAd expressing EGFP
DNA solutionRDB03938
AxCAEGFP-F40STAA
  Recombinant adenovirus expressing EGFP
Recombinant virusRDB03939
pAxCAEGFP-Fwt
  Shuttle vector to generate rAd expressing EGFP
DNA solutionRDB03943
AxCAEGFP-Fwt
  Recombinant adenovirus expressing EGFP
Recombinant virusRDB03944
pWEAxCAEGFP-F40S
  Shuttle vector to generate rAd expressing EGFP
DNA solutionRDB03945
AxCAEGFP-F40S
  Recombinant adenovirus expressing EGFP
Recombinant virusRDB03946
pCAEGFP
  Expression vector of EGFP
DNA solutionRDB03956
pRxhrGFP
  Shuttle vector to generate recombinant retrovirus expressing GFP
DNA solutionRDB03985
pCAEGFP
  Expression vector of GFP driven by CA promoter
DNA solutionRDB07631
pUAST-Notch-GFP
  P-element mediated gene transfer vector of Drosophila Notch
DNA solutionRDB08032
pUAST-GFPN-lacZ
  P-element mediated gene transfer vector of GFP-N and lacZ
DNA solutionRDB08035
pRS416-PADH1-ccdB-yEGFP-TTEF1
  Empty expression vector backbone of S. cerevisiae
DNA solutionRDB08380
pRS416-PADH1-yEGFP-TTEF1
  Empty expression vector backbone of S. cerevisiae
DNA solutionRDB08386
pRS416-PADH1-GAP1-yEGFP-TTEF1
  Expression clone of S. cerevisiae GAP1 cDNA
DNA solutionRDB08387
pRS416-PADH1-PUT4-yEGFP-TTEF1
  Expression clone of S. cerevisiae PUT4 cDNA
DNA solutionRDB08388
pRS416-PADH1-AGP1-yEGFP-TTEF1
  Expression clone of S. cerevisiae AGP1 cDNA
DNA solutionRDB08389
pRS416-PADH1-GNP1-yEGFP-TTEF1
  Expression clone of S. cerevisiae GNP1 cDNA
DNA solutionRDB08390
pRS416-PADH1-gap1[K9R/K16R]yEGFP-TTEF1
  Expression clone of S. cerevisiae GAP1 mutant [K9R/K16R] cDNA
DNA solutionRDB08391
pmAID-ER-FBG
  Shuttle vector to generate retrovector of mouse Aicda-ER
DNA solutionRDB08056
pUPAT-egfp3ic
  Gene trapping vector of mouse embryonic stem cell
DNA solutionRDB07884
pUPAT-SRV
  Gene trapping vector of mouse embryonic stem cell
DNA solutionRDB07885
pMT-PURO2G
  
DNA solutionRDB09004
pNHK60
  Expression clone for an auxin-based degron (AID) system
DNA solutionRDB08468
pN1-G-CaMP
  Genetically-encoded fluorescent calcium sensor cDNA
DNA solutionRDB06747
pN1-G-CaMP1.3
  Genetically-encoded fluorescent calcium sensor cDNA
DNA solutionRDB06748
pN1-G-CaMP1.6
  Genetically-encoded fluorescent calcium sensor cDNA
DNA solutionRDB06749
pN1-G-CaMP2
  Genetically-encoded fluorescent calcium sensor cDNA
DNA solutionRDB06750
AxCAwt-EGFP-VSV-G(ts045)
  Recombinant adenovirus expressing VSV glycoprotein tagged with GFP
Recombinant virusRDB07990
pPyCAG-EGFP-IP
  
DNA solutionRDB08525
pPyCAG-EGFP-IZ
  
DNA solutionRDB08526
pPyCAG-EGFP-ID
  
DNA solutionRDB08527
pPyCAG-EGFP-IB
  Expression clone of GFP under the control of CAG promoter, Blasticidin resistance
DNA solutionRDB08528
pBRPyCAG-EGFP-IH
  
DNA solutionRDB08529
pBRPyCAG-EGFP-IN
  
DNA solutionRDB08530
pRBS-EGFP
  Rbpj-binding sequence containing reporter GFP plasmid
DNA solutionRDB08039
pMx-E4BP4(Nfil3)
  Expression clone of mouse Nfil3 cDNA
DNA solutionRDB08379
pEGFP-hp38alpha
  Expression vector of human p38alpha MAPK cDNA, FLAG- and EGFP-tagged
DNA solutionRDB08179
pEGFP-hExip
  Plasmid vector of human p38alpha MAPK splicing variant cDNA, FLAG- and EGFP-tagged
DNA solutionRDB08188

clone_table2.pl-gfp_resourceen.csv

2013.06.23

2016.08.15