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It is one of the key issues to utilize the biomass for the worldwide energy crisis. The RIKEN BioResource Center (BRC) has collected numbers of genes that are related to cellulose saccharification and has constructed expression plasmids of those genes to express recombinant proteins in E. coli. Currently, 25 genes were collected from filamentous fungus Trichoderma reesei (Hypocrea jecorina: JCM 22676) and Pyrococcus furiosus (JCM 8422T). And then, 38 expression plasmids are also distributed. For example, recombinant endo-type cellulase EGII-His or EG-III-His was obtained from E. coli with the expression clone pET21c_Tr-egl2-His (RDB08289) or pET21c_Tr-egl3-His (RDB08290) which was subcloned the gene (egl2 or egl3) from T. reesei. EG2-His or EG3-His in crude extract indicated the activity at 12.2 U/ml and 4.6 U/ml, respectively (One ml of crude extract was prepared from 10 ml cultured E. coli). The information associated with plasmids, such as a nucleotide sequence and digestion pattern by restriction endonucleases. Furthermore, protocols of expression of recombinant proteins in E. coli are available on this website.
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Swollenins (Expansin-like proteins)