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January 23, 2015

Notice regarding human JUN cDNA pAxCALNLhc-jun(forward) cosmid clone

We, the RIKEN BioResource Center, regret to inform you that human JUN cDNA pAxCALNLhc-jun (forward) cosmid clone contains a defect.

The clone was constructed from the human JUN cDNA plasmid clone which was provided by a Japanese researcher. A fragment containing human JUN cDNA was isolated after restriction enzyme digestion and ligated into a cosmid vector. The presence of human JUN was confirmed by terminal end nucleotide sequences (about 500 bp of both ends) of the cosmid clone. The cosmid clone has been for distribution since 2005.

Recently, there was a request for this clone. The sequence analysis of the terminal ends revealed unfortunately that pAxCALNLhc-jun(forward) cosmid clone lacked 567 to 1703 nucleotide, 1137 bp missing (477 bp of 5' untranslated region and 660 bp of coding region corresponding human JUN gene NM_002228.3 of NCBI database, U.S.A), while it contains 72 to 566 nucleotide and 1704 to 2063 nucleotide. We are afraid that there was a possibility that researchers might not be able to obtain expected results because of this 1137 bp deletion. Therefore, we have decided to delete the pAxCALNLhc-jun (forward) cosmid clone from the web catalog. There is an alternative human JUN cDNA clone in our stock.

We have sent a note to the past users by December 22, 2014, expressing our sincere apology and explaining this matter. In the same note, we also offered an alternative human cDNA to the user.

Recent improvement of sequence analysis of clones makes the great expansion of readable sequences. The advanced technology may reveal that we happened to have unintentionally distributed some resources with defects, such as this clone. We will keep doing our best to improve the quality of our resources to contribute to life science research. We ask you for your understanding and continuous support.

If you have any question and concern on this matter, please feel free to contact us (dnabank@brc.riken.jp).


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(T.M.)