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Laboratory use only.
Not intended for intentional introduction into the environment.


catalog no.Name of cloneRunning titleClassCloning siteSeq file
RDB02136S-HA-pRc/CMVExpressing HA-tagged fusion protein, CMV promoterVectorRDB2136.pdfRDB2136z.seq
RDB02139D-HA-pRc/CMVExpressing a fusion protein tagged with duplicated HA, CMV promoterVectorRDB2139.pdfRDB2139z.seq
RDB02137S-T7-pRc/CMVExpressing T7-tagged fusion protein, CMV promoterVectorRDB2137.pdfRDB2137z.seq
RDB02138D-T7-pRc/CMVExpressing a fusion protein tagged with duplicated T7, CMV promoterVectorRDB2138.pdfRDB2138z.seq
RDB02140S-Myc-pRc/CMVExpressing Myc-tagged fusion protein, CMV promoterVectorRDB2140.pdfRDB2140z.seq
RDB02141D-Myc-pRc/CMVExpressing a fusion protein tagged with duplicated Myc epitopes, CMV promoterVectorRDB2141.pdfRDB2141z.seq

Expression vectors listed above are constructed by Dr. Yoshihiro Takemoto of Tokyo Medical and Dental University and published in the DNA Cell Biol. 16 (7): 893-896, 1997. You can select one or two copies of epitope tags among the HA, T7 and Myc. The NotI restriction enzyme site is enable you to create expression vectors tagged your gene of interest with these vectors. After the cloning of your gene, the NotI site also allows inserted gene to be moved to another vectors among these 6 vectors.
Please visit the following site for genetic materials deposited to our division by Dr. Takemoto.
http://dna.brc.riken.jp/search/dep3357.html

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catalog no.Name of cloneRunning titleClassCloning siteSeq file
RDB06083pRSV_S-FLAGExpressing FLAG-tagged fusion protein, RSV promoterVectorRDB6083.pdfRDB6083z.seq

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catalog no.Name of cloneRunning titleClassCloning siteSeq file
RDB05956pCMV_S-FLAGExpressing FLAG-tagged fusion protein, CMV promoterVectorRDB5956.pdfRDB5956z.seq
RDB06072pCMFlag_lacZExpression vector of lacZ, with FLAG-tag, control vectorDNA cloneRDB6072.pdf 
RDB06071pCMFlag_EGFPExpression vector of GFP, with FLAG-tag, control vectorDNA cloneRDB6072.pdf 

pCMV_S-FLAG vector is a mammalian expression vector designed to express proteins with FLAG epitope tag (Met-DYKDDDDK). The NotI restriction enzyme site is enable you to create expression vectors tagged your gene of interest. The first ATG of the FLAG tag is included in the NcoI restriction enzyme site (CCATGG) and the NcoI site is available for cloning in-frame the FLAG tag and your interested gene together into bacterial expression vectors. The EcoRI site abutting upon the FLAG tag allows to excite your interested gene with the FLAG tag. PCR products starting with ATG codon can be cloned in-flame into the blunted NotI restriction site.




catalog no.Name of cloneRunning titleClassCloning siteSeq file
RDB07396pCMV_s-FLAGcDestination vector to generate eukaryotic expression vector, with FLAG-tagVectorRDB7396.pdfRDB7396z.seq
pCMV_s-FLAGc vector is a derivative of the pCMV_S-FLAG vector. Gateway® technology offers one-step cloning of your interested gene in Entry clones into the pCMV_s-FLAGc expression vector. Please refer the following website for Entry clones from the Genome Network Project Human Full-Length cDNA.
http://dna.brc.riken.jp/en/GNPcloneen.html

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(2007.12.05. T.M.)

2015.10.25