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Expression Vector Backbone of Saccharomyces cerevisiae

Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins.
Myc-tag (EQKLISEEDL) can be recognized by mouse monoclonal anitbody 9E10.
Depositor: Dr. Hidekatsu Iha
Reference: Biotechniques, 25, 936-938 (1998)., PMID 9863043.

Catalog no.NameTagOriMarkerPromoterSize (kb)Usable sites
RDB01947pAMH10Myccentromere-typeHIS3ADH14.56
RDB01948pAMH20Myc2micronHIS3ADH15.36
RDB01949pAML10Myccentromere-typeLEU2ADH15.16
RDB01950pAML20Myc2micronLEU2ADH15.96
RDB01951pAMT10Myccentromere-typeTRP1ADH14.35
RDB01952pAMT20Myc2micronTRP1ADH15.15
RDB01953pAMU10Myccentromere-typeURA3ADH14.66
RDB01954pAMU20Myc2micronURA3ADH15.46
RDB01955pGMH10Myccentromere-type HIS3GAL1-104.86
RDB01956pGMH20Myc2micronHIS3GAL1-105.66
RDB01957pGML10Myccentromere-typeLEU2GAL1-105.46
RDB01958pGML20Myc2micron LEU2GAL1-106.26
RDB01959pGMT10Myccentromere-typeTRP1GAL1-104.65
RDB01960pGMT20Myc2micronTRP1GAL1-105.45
RDB01961pGMU10Myccentromere-type URA3GAL1-104.96
RDB01962pGMU20Myc2micronURA3GAL1-105.76

Empty expression vector backbone of S. cerevisiae to express GFP-tagged protein.
Depositor: Dr. Hiroshi Takagi
Reference: Genes Cells, 18 (6): 459-475 (2013)., PMID 23517290.

Catalog no.NameRunning title
RDB08380pRS416-PADH1-ccdB-yEGFP-TTEF1Empty expression vector backbone of S. cerevisiae.Gateway(R) destination vector.Contains ccdB and use DB3.1 or an equivalent host bacteria.
RDB08386pRS416-PADH1-yEGFP-TTEF1Empty expression vector backbone of S. cerevisiae

National BioResource Project - Yeast
Strains and plasmids of budding yeast (Saccharomyces cerevisiae) is available.


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(2008.05.20 T.M.)

2015.09.25