Shuttle-vector system for S. cerevisiae-E. coli

Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins

RDB no.	Name	Running title	Type	Tag	Ori	Marker	Promoter	Size (kb)	Usable sites
1947	pAMH10	E. coli - S. cerevisiae shuttle vector	Vector	Myc	centromere-type	HIS3	ADH1	4.5	6
1948	pAMH20	E. coli - S. cerevisiae shuttle vector	Vector	Myc	2micron	HIS3	ADH1	5.3	6
1949	pAML10	E. coli - S. cerevisiae shuttle vector	Vector	Myc	centromere-type	LEU2	ADH1	5.1	6
1950	pAML20	E. coli - S. cerevisiae shuttle vector	Vector	Myc	2micron	LEU2	ADH1	5.9	6
1951	pAMT10	E. coli - S. cerevisiae shuttle vector	Vector	Myc	centromere-type	TRP1	ADH1	4.3	5
1952	pAMT20	E. coli - S. cerevisiae shuttle vector	Vector	Myc	2micron	TRP1	ADH1	5.1	5
1953	pAMU10	E. coli - S. cerevisiae shuttle vector	Vector	Myc	centromere-type	URA3	ADH1	4.6	6
1954	pAMU20	E. coli - S. cerevisiae shuttle vector	Vector	Myc	2micron	URA3	ADH1	5.4	6
1955	pGMH10	E. coli - S. cerevisiae shuttle vector	Vector	Myc	centromere-type	HIS3	GAL1-10	4.8	6
1956	pGMH20	E. coli - S. cerevisiae shuttle vector	Vector	Myc	2micron	HIS3	GAL1-10	5.6	6
1957	pGML10	E. coli - S. cerevisiae shuttle vector	Vector	Myc	centromere-type	LEU2	GAL1-10	5.4	6
1958	pGML20	E. coli - S. cerevisiae shuttle vector	Vector	Myc	2micron	LEU2	GAL1-10	6.2	6
1959	pGMT10	E. coli - S. cerevisiae shuttle vector	Vector	Myc	centromere-type	TRP1	GAL1-10	4.6	5
1960	pGMT20	E. coli - S. cerevisiae shuttle vector	Vector	Myc	2micron	TRP1	GAL1-10	5.4	5
1961	pGMU10	E. coli - S. cerevisiae shuttle vector	Vector	Myc	centromere-type	URA3	GAL1-10	4.9	6
1962	pGMU20	E. coli - S. cerevisiae shuttle vector	Vector	Myc	2micron	URA3	GAL1-10	5.7	6

Myc-tag (EQKLISEEDL) can be recognized by mouse monoclonal anitbody 9E10.

Depositor: Dr. Hidekatsu Iha
Reference: Biotechniques 25 (6): 936-938, 1998.

Strains and plasmids of budding yeast (Saccharomyces cerevisiae) is available.

2008.05.20