Calcium ion sensor clones
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Calcium Ion Sensor Clones

probe nameCa2+ affinity (Kd, nM)dynamic range (Fmax/Fmin)features&applicationrefferenceplasmid clone
(cat. num)
G-CaMP1.61464.9Brightness improved version. Work at 25ºC (hippocampal slice of rat) to 28ºC (HEK293 cell, HeLa cell) but not at 37ºC (HEK293 cell).PMID 11175727, 16159115, 16537386pN1-G-Camp1.6 (RDB06749)
G-CaMP21463.9 to 4.9Heat stable version. Work at 37ºC (HEK293 cell, heart of mouse embryo, primary culture of hippocampal astrocytes).PMID 16537386, 22472649pN1-G-CaMP2 (RDB06750)
G-CaMP-HS1024.1High-Ca2+ affinity version (ie: mammalian cell, zebrafish).PMID 21383146, 27239939G-CaMP-HS (RDB14607)
G-CaMP615811.4 to 27.9Sensitivity improved version. Quick ON/OFF response allow to observe neuronal-cell calcium spikes (ie: mouse brain section, mouse B cell, C. elegans).PMID 23240011, 27385517, 27428827G-CaMP6 (RDB14609)
pMXs-IRES-hNGFR_G-CaMP6 (RDB14513)
G-CaMP7.0921219.3Enhanced brightness allow to observe flashing fluorescence of iPS cell-derived cardiomyocytes of cynomolgus monkey.PMID 27723741G-CaMP7.09 (RDB14612)

G-CaMP clones

G-CaMP Ca2+ sensor, which is composed with calmodulin, split GFP and M13 peptide (CaM binding domain of myosin light chain kinase), is designated to visualize intracellular [Ca2+] dynamics (Dr. Jin-ichi Nakai, Saitama University).

Various improvements of the G-CaMP have been made for optimum temperature, signal/noise ratio, susceptibility and so on. These improved G-CaMPshave been leading fluorescent calcium indicators and used by researchers around the world. Many papers using G-CaMPs have been published reporting the estimation of calcium concentration, for example, in the cardiomyocytes differentiated from iPS cells (Shiba, Y. et al., 2016; cat.no. RDB14612 G-CaMP7.09) and in neurons of zebrafish (Muto, A. et al., 2011; cat.no. RDB14607 G-CaMP-HS).

DNA Resource

Catalog #Name of clonePublication
RDB06747pN1-G-CaMPNakai, J., A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. Nat. Biotechnol., 19, 137-141 (2001). PMID 11175727.
RDB06748pN1-G-CaMP1.3Nakai, J., A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. Nat. Biotechnol., 19, 137-141 (2001). PMID 11175727.
RDB06749pN1-G-CaMP1.6Ohkura, M., Genetically encoded bright Ca2+ probe applicable for dynamic Ca2+ imaging of dendritic spines. Anal. Chem., 77, 5861-5869 (2005). PMID 16159115.
RDB06750pN1-G-CaMP2Tallini, Y.N., Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+ indicator GCaMP2. Proc. Natl. Acad. Sci. U S A., 103, 4753-4758 (2006). PMID 16537386.
RDB14606G-CaMP4.1Shindo, A., Tissue-tissue interaction-triggered calcium elevation is required for cell polarization during Xenopus gastrulation. PLoS One 5 (2): e8897 (2010). PMID 20126393.
RDB14607G-CaMP-HSMuto, A., Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish. Proc. Natl. Acad. Sci. U.S.A. 108 (13): 5425-5430 (2011). PMID 21383146.
RDB14609G-CaMP6Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.
RDB14610G-CaMP7Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.
RDB14611G-CaMP8Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.
RDB14612G-CaMP7.09Shiba, Y., Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Nature 538 (7625): 388-391 (2016). PMID 27723741.

Related Articles

Misa Arizono, Hiroko Bannai, Kyoko Nakamura, Fumihiro Niwa, Masahiro Enomoto, Toru Matsu-ura, Akitoshi Miyamoto, Mark W. Sherwood, Takeshi Nakamura, and Katsuhiko Mikoshiba
“Receptor-Selective Diffusion Barrier Enhances Sensitivity of Astrocytic Processes to Metabotropic Glutamate Receptor Stimulation”.
Science Signaling ,2012.
doi: 10.1126/scisignal.2002498

Press Release (2012, in Japanese)
Press Release (2008, in Japanese)

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(GRP0029e 2012.04.06 T.M. )


2017.07.15