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BAC Clone Set

C57BL/6N (B6N) mouse BAC clone

General Information

The Gene Engineering Division has constructed a BAC library of the B6N substrain and the RIKEN BioResource Center accomplished BAC end sequencing of B6N and register the sequence data with DDBJ in a collaboration with the National Institute of Genetics under the Genome Information Upgrading Program of MEXT NBRP. The B6N Mouse BAC library consisted of 128,000 clones representing 90.2% of actual coverage of haploid genome.
The germ cell competent C57BL/6N ES cell lines are available from the Cell Engineering Division of RIKEN BRC.

Forms for Distribution

Please find ID of individual clone(s) using the Mouse BAC browser.
Mouse BAC browser B6N BAC
(open in a new window)

Forms required

Forms Description
Form A Order Form
Please specify ID of clone(s) ex. B6Ng01-122P06.

Please complete the form with your shipping information including your account number of an international courier (FedEx. World Courier, TNT Express, DHL Global Forwarding and others).
Form C Material Transfer Agreement
We would like to ask a signature of the Authorized Representative of a recipient institution.

Please indicate "NBRP B6N mouse BAC clone" as the BIOLOGICAL RESOURCE.

In the the Section 2(a), please write your purpose of use of clone in 10 to 20 words
Please put in the terms and condisions (Section 4) that
"In publishingthe research results to be obtained by use of the B6N mouse BAC clone, an acknowledgment to the RIKEN BioResource Center is requested."
(Please e-mail to dnabank@brc.riken.jp to obtain MTA in MS-Word format)
    Send forms:
      The DNA Bank, RIKEN BioResource Center,
      3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
      Fax: (+81)-29-836-9120
      e-mail: dnabank@brc.riken.jp
    Further information for sale.

Personal data protection policy
For protection of personal data at RIKEN BioResource Center, please reffer Personal data protection policy.

  • B6N mouse BAC clone(s) is shipped as stab agar culture with 12.5 ug/mL chloramphenicol in an individual tube.
  • When you receive the plastic vial of stab culture, please streak the bacteria on an LB agar plate containing 12.5 ug/mL chloramphenicol. We hope you can find colonies.
  • When you streak bacteria, please take bacteria from the middle part of stab agar medium but not those from the surface of the stab which are not really healthy.
  • The vial should be placed in a refrigerator (4 C degree) but not in a freezer. Stab culture is not meant to be for frozen storage of bacteria.

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(T.M. 2010.05.06)