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BAC Clone Library

C57BL/6N (B6N) mouse BAC clone

General Information

The Gene Engineering Division has constructed a BAC library of the B6N substrain and the RIKEN BioResource Center accomplished BAC end sequencing of B6N and register the sequence data with DDBJ in a collaboration with the National Institute of Genetics under the Genome Information Upgrading Program of MEXT NBRP. The B6N Mouse BAC library consisted of 128,000 clones representing 90.2% of actual coverage of haploid genome.
The germ cell competent C57BL/6N ES cell lines are available from the Cell Engineering Division of RIKEN BRC.

Forms for Distribution

Please find ID of individual clone(s) using the Mouse BAC browser.
Mouse BAC browser B6N BAC
(open in a new window)

Forms required

Forms Description
Form A Order Form
Specify ID of clone(s) ex. B6Ng01-122P06 in the Name of clone column.
Leave Catalog no. column empty.
Complete the form with your shipping information including your account number of an international courier (FedEx. World Courier, TNT Express and others).
Form C Material Transfer Agreement
We would like to ask a signature of the Authorized Representative of a recipient institution.

Indicate "NBRP B6N mouse BAC clone" as the BIOLOGICAL RESOURCE.

In the the Section 2(a), please write your purpose of use of clone in 10 to 20 words
Please put in the terms and condisions (Section 4) that
"In publishingthe research results to be obtained by use of the B6N mouse BAC clone, an acknowledgment to the RIKEN BioResource Center is requested."
    Send forms:
      The DNA Bank, RIKEN BioResource Center,
      3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
      Fax: (+81)-29-836-9120
      e-mail: dnabank.brc@riken.jp (Please e-mail us to ask MTA in MS-Word format)
    Further information for sale.

Personal data protection policy
For protection of personal data at RIKEN BioResource Center, please reffer Personal data protection policy.

  • B6N mouse BAC clone(s) is shipped as stab agar culture with 12.5 ug/mL chloramphenicol in an individual tube.
  • When you receive the plastic vial of stab culture, please streak the bacteria on an LB agar plate containing 12.5 ug/mL chloramphenicol. We hope you can find colonies.
  • When you streak bacteria, please take bacteria from the middle part of stab agar medium but not those from the surface of the stab which are not really healthy.
  • The vial should be placed in a refrigerator (4 C degree) but not in a freezer. Stab culture is not meant to be for frozen storage of bacteria.

Data Sheet

C57BL/6N (B6N) mouse BAC clone
Strain information
SpeciesMus musculus domesticus
StrainC57BL/6NCrlCrlj, male
Clone information
Vector pBACe3.6 (Cmr, U80929), EcoRI site
InsertEcoRI partial digestion
HostDH10B, E. coli
Sequence primers5' sequence by T7 primer: TGACATTGTAGGACTATATTGC
3' sequence by TJ primer: ATCTGCCGTTTCGATCCTCC
Nucleotide SequencesDNA Databank of Japan (DDBJ),
DH839446 to DH961576, and
GA000001 to GA131507
Total number of clones 128,007
Actual Genome Coverageca. 90.2%

Related Information

Please visit Information site

  • Articles Published by Using This Materials
  • References and tips
  • Related products

NIG Mouse Genome Database http://molossinus.lab.nig.ac.jp/msmdb/BacDetail

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(T.M. 2010.05.06)