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BioResource Center RIKEN BRC DNA BANK
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Genomic DNA

JCM Microbial Genomic DNA

General Information

Bacteria and archaeans are cultured in the RIKEN BRC-JCM and their DNA are prepared in the Gene Engineering Division. High molecular weight DNA is prepared by phenol-chloroform extraction. The DNA is suitable for amplification by PCR. The concentration and purity of DNA samples are determined spectrophotometrically and further checked by PCR amplification of 16S rRNA gene and following electrophoresis. Nucleotide sequences are also confirmed. Shipping amout is 1 μg per vial and DNA concentrations generally range from 50 to 100 ng/μl. (The concentrations of individual samples are supplied with a data sheet.) The DNAs are stored in TE buffer (10 mM TRIS pH7.5, 1mM EDTA) at -30oC.

Attention International Transfer - "Veterinary Permission" or "Permit to Import Quarantine Material" may be required for shipment of the biological materials. Please follow guide lines in your country.

Forms for Distribution

Forms required

Forms Description
Form A Order Form
Please specify strain names with JGD numbers (catalog number).
Form C Material Transfer Agreement
Please put the terms and condisions that is indicated in the individual data sheet of the genomic DNA listed here.
(Please e-mail to dnabank@brc.riken.jp to obtain MTA in MS-Word format)

Distribution fee per vial (one microgram):
17,000 YEN (for academic institutions).
34,000 YEN (for profit organizations).
(Shipping cost is not included.)

Please visit this page for further information of distribution and fees.

    If you are inquiring about the availability of a specific DNA, please give as much information about the strain stock number as possible.
    For more information contact:
    [Regarding DNA] [Regarding bacteria strains]
    Gene Engineering Division,
    RIKEN BioResource Center
    3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
    Fax: +81-29-836-9120
    Microbe Division / Japan Collection of Microorganisms
    RIKEN BioResource Center
    3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
    Fax: +81-29-836-9561

    The availability of some DNAs is limited. All requests for these DNAs will be given due consideration, but we cannot assure availability.

Personal data protection policy
For protection of personal data at RIKEN BioResource Center, please reffer Personal data protection policy.

Related Information

Reconstitution of DNA

  • In the case you received plastic vials contains precipitated DNA with ethanol.
  • A precipitant might stick to the lid or wall during transportation.
  • Before opening the tube, please spin down a minute or two at 5,000 g (~7,000 rpm, table top microfuge).
  • Please remove ethanol with pipet (DO NOT tooch precipitate!)
  • Immediately after removal of ethanol resuspend the DNA in an appropriate amount of TE buffer (10 mM Tris-Cl, pH 7.5, 1 mM EDTA), and dissolve the DNA pellet by gently tapping with fingers (DO NOT shake vigorously! A little amount of ethanol won't affect to many applications).
  • If the DNA is difficult to solve, leave the vial at 4 degreeC overnight to allow the DNA to dissolve.
  • Once reconstituted in TE buffer, the DNA should be stored at -30 degreeC.

Articles Published by Using This Materials

  • Kobayashi, K., Kikuno, I., Kuroha, K., Saito, K., Ito, K., Ishitani, R., Inada, T., Nureki, O. Structural basis for mRNA surveillance by archaeal Pelota and GTP-bound EF1α complex. Proc. Natl. Acad. Sci. U S A., 107 (41): 17575-17579 (2010). 20876129
  • Saito, K., Kobayashi, K., Wada, M., Kikuno, I., Takusagawa, A., Mochizuki, M., Uchiumi, T., Ishitani, R., Nureki, O., Ito, K. Omnipotent role of archaeal elongation factor 1 alpha (EF1α in translational elongation and termination, and quality control of protein synthesis. Proc. Natl. Acad. Sci. U S A., 107 (45): 19242-19247 (2010). 20974926
  • Kobayashi, K., Saito, K., Ishitani, R., Ito, K., Nureki, O. Structural basis for translation termination by archaeal RF1 and GTP-bound EF1alpha complex. Nucleic Acids Res., 40 (18): 9319-9328 (2012). 22772989
  • Tanaka, Y., Hipolito, C.J., Maturana, A.D., Ito, K., Kuroda, T., Higuchi, T., Katoh, T., Kato, H.E., Hattori, M., Kumazaki, K., Tsukazaki, T., Ishitani, R., Suga, H., Nureki, O. An archaeal homolog of proteasome assembly factor functions as a proteasome activator. PLoS One, 8 (3): e60294 (2013). 23535598
  • Kumoi, K., Satoh, T., Murata, K., Hiromoto, T., Mizushima, T., Kamiya, Y., Noda, M., Uchiyama, S., Yagi, H., Kato, K. An archaeal homolog of proteasome assembly factor functions as a proteasome activator. PLoS One, 8 (3): e60294 (2013). 23555947
  • Nishizawa, T., Kita, S., Maturana, A.D., Furuya, N., Hirata, K., Kasuya, G., Ogasawara, S., Dohmae, N., Iwamoto, T., Ishitani, R., Nureki, O. Structural basis for the counter-transport mechanism of a H+/Ca2+ exchanger. Science, 341 (6142): 168-172 (2013). 23704374

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