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Gene Set Collection

p53 dependent G2 arrest


DNA damaging agents induces accumulation and stimulation of p53 protein,which activate or inhibit several gene expression.
p53 activates p21,14-3-3 sigma and Gadd45 transcription. p21 binds Cyclin B/cdc2 complex to keep it inactive form. 14-3-3 sigma is a cytoplasmic protein that binds to Cyclin B/cdc2 complex to let it stay in cytoplasm, therefore Cyclin B/cdc2 can not act as an active complex. Gadd45 inhibits formation of Cyclin B/cdc2 complex.
p53 also inhibits G2-M transition by repressing the transcription of cdc2 and CyclinB. (Contributed by Dr. Koji Nakade)


Reporters

Catalog no.Name of cloneShort description
RDB13080pCAG-Fucci2aFucci2a cell cycle reporter.
RDB13081pROSA-floxNeo-Fucci2aFucci2a cell cycle reporter. Targeting vector for R26 locus.

LocusRDB no.TypeName of cloneRunning title
Trp531285DNA clonep53-LTR pUC18 (Anti Sense#9)Expression vector, Antisense p53, cDNA
Trp531284DNA clonep53-LTR pUC18 (Sense#3)Expression vector, Sense p53, cDNA
TP535507DNA clonepKM2L-phP53Promoter Bank clone, Human p53 promoter
TP533187Recombinant adenovirusAxCAhp53KP1065Recombinant adenovirus harboring human p53 cDNA (Ser96Pro)
TP533186Recombinant adenovirusAxCAhp53KP1010Recombinant adenovirus harboring human p53 cDNA (Gly105Asp)
TP533185Recombinant adenovirusAxCAhp53KP1007Recombinant adenovirus harboring human p53 cDNA (Gly374Cys)
TP533184Recombinant adenovirusAxCAhp53KP1094Recombinant adenovirus harboring human p53 cDNA (Pro89His)
TP533183Recombinant adenovirusAxCAhp53KP1070Recombinant adenovirus harboring human p53 cDNA (Ser240Asn)
TP532807Recombinant adenovirusAxCALNLhp53R273H (forward)Recombinant adenovirus harboring human p53 cDNA (R273H)
TP532802Recombinant adenovirusAxCALNLhp53.clone 28 (forward)Recombinant adenovirus harboring human p53 cDNA (deletion)
TP532799Recombinant adenovirusAxCALNLhp53P70 (forward)Recombinant adenovirus harboring human p53 cDNA (Pro128Thr)
TP532798Recombinant adenovirusAxCALNLhp53P68 (forward)Recombinant adenovirus harboring human p53 cDNA
TP532795Recombinant adenovirusAxCALNLhp53WT (forward)Recombinant adenovirus harboring human p53 cDNA
TP532785Recombinant adenovirusAxCAhp53KP1070 (forward)Recombinant adenovirus harboring human p53 cDNA (Ser240Asn)
TP532784Recombinant adenovirusAxCAhp53KP1065 (forward)Recombinant adenovirus harboring human p53 cDNA (Ser96Pro)
TP532783Recombinant adenovirusAxCAhp53KP1010 (forward)Recombinant adenovirus harboring human p53 cDNA (Gly105Asp)
TP532782Recombinant adenovirusAxCAhp53KP1007 (forward)Recombinant adenovirus harboring human p53 cDNA (Gly374Cys)
TP532771Recombinant adenovirusAxCAhp53.clone 28 (forward)Recombinant adenovirus harboring human p53 cDNA (deletion)
TP532757Recombinant adenovirusAxCAhp53P70 (forward)Recombinant adenovirus harboring human p53 cDNA (Pro128Thr)
TP532756Recombinant adenovirusAxCAhp53P68 (forward)Recombinant adenovirus harboring human p53 cDNA
TP532755Recombinant adenovirusAxCAhp53WT (forward)Recombinant adenovirus harboring human p53 cDNA
TP532713ShuttleVectorpAxCALNLhp53R273H (reverse)Shuttle vector to produce rAd expressing human p53 (R273H)
TP532712ShuttleVectorpAxCALNLhp53R273H (forward)Shuttle vector to produce rAd expressing human p53 (R273H)
TP532630ShuttleVectorpAxCAhp53R273H (reverse)Shuttle vector to produce rAd expressing human p53 (R273H)
TP532629ShuttleVectorpAxCAhp53R273H (forward)Shuttle vector to produce rAd expressing human p53 (R273H)
TP532509ShuttleVectorpAxCAhp53P26 (reverse)Shuttle vector to generate rAd harboring human p53
TP532508ShuttleVectorpAxCAhp53P26 (forward)Shuttle vector to produce rAd expressing human p53
TP532507ShuttleVectorpAxCAhp53P25 (reverse)Shuttle vector to generate rAd harboring human p53
TP532506ShuttleVectorpAxCAhp53P25 (forward)Shuttle vector to produce rAd expressing human p53
TP532505ShuttleVectorpAxCAhp53P24 (reverse)Shuttle vector to generate rAd harboring human p53
TP532504ShuttleVectorpAxCAhp53P24 (forward)Shuttle vector to produce rAd expressing human p53
TP532503ShuttleVectorpAxCAhp53P21 (reverse)Shuttle vector to generate rAd harboring human p53
TP532502ShuttleVectorpAxCAhp53P21 (forward)Shuttle vector to produce rAd expressing human p53
TP532501ShuttleVectorpAxCAhp53P19 (reverse)Shuttle vector to generate rAd harboring human p53
TP532500ShuttleVectorpAxCAhp53P19 (forward)Shuttle vector to produce rAd expressing human p53
TP532499ShuttleVectorpAxCAhp53P17 (reverse)Shuttle vector to generate rAd harboring human p53
TP532498ShuttleVectorpAxCAhp53P17 (forward)Shuttle vector to produce rAd expressing human p53
TP532497ShuttleVectorpAxCAhp53P16 (reverse)Shuttle vector to generate rAd harboring human p53
TP532496ShuttleVectorpAxCAhp53P16 (forward)Shuttle vector to produce rAd expressing human p53
TP532495ShuttleVectorpAxCAhp53P50 (reverse)Shuttle vector to generate rAd harboring human p53
TP532494ShuttleVectorpAxCAhp53P50 (forward)Shuttle vector to produce rAd expressing human p53
TP532493ShuttleVectorpAxCAhp53P13 (reverse)Shuttle vector to generate rAd harboring human p53
TP532492ShuttleVectorpAxCAhp53P13 (forward)Shuttle vector to produce rAd expressing human p53
TP532437DNA clonepCAGhp53R273H (Reverse)Mammalian vector expressing mutant of p53 gene (R273H)
TP532436DNA clonepCAGhp53P70 (Reverse)Mammalian vector expressing mutant of p53 gene (Pro128Thr)
TP532435DNA clonepCAGhp53P68 (Forward)Mammalian vector expressing mutant of p53 gene (Ile 162 Val, Gly 266 Asp, frame shift)
TP532434DNA clonepCAGhp53WT (Reverse)Mammalian vector expressing wild type of p53 gene
TP532433DNA clonepCAGhp53R273H (Forward)Mammalian vector expressing mutant of p53 gene (R273H)
TP532432DNA clonepCAGhp53clone28 (Forward)Mammalian vector expressing the deletion mutant of p53 gene
TP532431DNA clonepCAGhp53P70 (Forward)Mammalian vector expressing mutant of p53 gene (Pro128Thr)
TP532430DNA clonepCAGhp53P68 (Reverse)Mammalian vector expressing mutant of p53 gene (Ile 162 Val, Gly 266 Asp, frame shift)
TP532429DNA clonepCAGhp53WT (Forward)Mammalian vector expressing wild type p53 gene
TP531982Recombinant AdenovirusAxCALNLhp53Recombinant adenovirus expressing human p53
GADD45B5377ShuttleVectorpAxCALNLhGADD45B(reverse)Shuttle vector to generate rAd harboring GADD45B Sugano full-length cDNA clone
GADD45B5376ShuttleVectorpAxCALNLhGADD45B(forward)Shuttle vector to generate rAd harboring GADD45B Sugano full-length cDNA clone
GADD45B5029ShuttleVectorpAxCALNLhGADD45B(reverse)Shuttle vector to generate rAd expressing human GADD45B
GADD45B5028ShuttleVectorpAxCALNLhGADD45B(forward)Shuttle vector to generate rAd expressing human GADD45B
GADD45B4151ShuttleVectorpAxCALNLhGADD45BShuttle vector to generate rAd harboring Sugano full-length cDNA clone
GADD45B3584ShuttleVectorpAxCALNLhGADD45B(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CDKN1A5893DNA clonepKM2L-phP21Promoter Bank clone, Human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 promoter
CDKN1A3576ShuttleVectorpAxCALNLhp21(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CDKN1A1999ShuttleVectorpAxCAhp21Shuttle vector to generate rAd expressing human CDKI p21
CDK74997ShuttleVectorpAxCALNLhCDK7(reverse)Shuttle vector to generate rAd expressing human CDK7
CDK74996ShuttleVectorpAxCALNLhCDK7(forward)Shuttle vector to generate rAd expressing human CDK7
CDK74113ShuttleVectorpAxCALNLhCDK7Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CDK74087ShuttleVectorpAxCALNLhCDK7Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CDK73539ShuttleVectorpAxCALNLhCDK7(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CDK73503ShuttleVectorpAxCALNLhCDK7(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
Cdk76948H3002H05Cdk7, NIA/NIH Mouse 15K cDNA Clone (full CDS)
cdc21570DNA clonepIBI delta Xe-cdc2-33RXenopus cdc2 (kinase-deficient) cDNA
cdc21569DNA clonepIBI delta Xe-cdc2Xenopus cdc2 cDNA
CCNH6416DNA clonepCMFlag_hsCCNHExpression vector of human CCNH
CCNH4093ShuttleVectorpAxCALNLhCyclin HShuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCNH3510ShuttleVectorpAxCALNLhCyclin H(forward)Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCND34589DNA cloneSEREX clone NGO-Br-20 (ID 859) #1SEREX clone NGO-Br-20 (ID 859) #1

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(GSB0013e, T.M. 2004.04.05)

2014.08.29